breseq  version 0.35.4  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 205 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*190,4240CA77.0% 303.6 / 100.5 178A13D (GCC→GAC) unknownhypothetical protein
*1693,7770A.73.3% 21.4 / 11.2 15coding (26/921 nt)unknownGlycosyl transferase, family 2
*1693,7780T.73.3% 21.2 / 11.4 15coding (25/921 nt)unknownGlycosyl transferase, family 2
*1693,7790T.73.3% 21.2 / 11.4 15coding (24/921 nt)unknownGlycosyl transferase, family 2
*1693,7800G.73.3% 21.5 / 12.9 15coding (23/921 nt)unknownGlycosyl transferase, family 2
*11,333,3990A.61.2% 25.5 / 137.3 85intergenic (+39/‑14)unknown/unknowntRNA‑Leu‑TAA/tRNA‑Lys‑TTT
*11,333,4000G.60.5% 13.5 / 155.7 86intergenic (+40/‑13)unknown/unknowntRNA‑Leu‑TAA/tRNA‑Lys‑TTT
*11,333,6270CA60.0% 32.4 / 54.0 50S22Y (TCT→TAT) unknownChromosome partitioning ATPase in PFGI‑1‑like cluster, ParA‑like
*11,063,9700AC57.4% 19.4 / 55.6 54T1108P (ACC→CCC) unknownChromosome partition protein MukB
*1225,2060A.54.5% 7.1 / 16.9 11coding (119/375 nt)unknownhypothetical protein
*1225,2070G.54.5% 7.6 / 19.6 11coding (118/375 nt)unknownhypothetical protein
*11,333,4881.A50.0% 48.9 / 230.9 106noncoding (76/76 nt)unknowntRNA‑Lys‑TTT
*11,333,4882.G50.0% 57.0 / 251.2 106noncoding (76/76 nt)unknowntRNA‑Lys‑TTT
*1630,4740TG49.2% 56.4 / 24.9 66L54V (TTG→GTG) unknownN‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9)
*11,333,5530TC48.4% 7.4 / 96.5 93intergenic (+65/‑10)unknown/unknowntRNA‑Lys‑TTT/Chromosome partitioning ATPase in PFGI‑1‑like cluster, ParA‑like
*11,333,5180AG47.6% 20.0 / 107.2 103intergenic (+30/‑45)unknown/unknowntRNA‑Lys‑TTT/Chromosome partitioning ATPase in PFGI‑1‑like cluster, ParA‑like
*11,333,5660TA47.1% 51.2 / 124.5 87C2S (TGC→AGC) unknownChromosome partitioning ATPase in PFGI‑1‑like cluster, ParA‑like
*11,333,5690AG47.1% 17.4 / 86.9 87I3V (ATT→GTT) unknownChromosome partitioning ATPase in PFGI‑1‑like cluster, ParA‑like
*11,333,5000CT47.1% 28.5 / 96.3 102intergenic (+12/‑63)unknown/unknowntRNA‑Lys‑TTT/Chromosome partitioning ATPase in PFGI‑1‑like cluster, ParA‑like
*11,333,5021.A47.1% 70.5 / 206.6 102intergenic (+14/‑61)unknown/unknowntRNA‑Lys‑TTT/Chromosome partitioning ATPase in PFGI‑1‑like cluster, ParA‑like

Marginal new junction evidence (lowest skew 10 of 72 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? 12 = 4860 (0.000)9 (0.080) 4/430 3.2 100% coding (486/486 nt) unknown 3'(2'),5'‑bisphosphate nucleotidase (EC 3.1.3.7)
?4 1 = 0 (0.000)coding (135/135 nt) unknown 3'(2'),5'‑bisphosphate nucleotidase (EC 3.1.3.7)
* ? 1 1009324 =4 (0.050)22 (0.300) 15/376 3.2 92.6% intergenic (+172/‑89) unknown/unknown Threonyl‑tRNA synthetase (EC 6.1.1.3)/Type III restriction‑modification system methylation subunit (EC 2.1.1.72)
?1 = 1009313 0 (0.000)intergenic (+161/‑100) unknown/unknown Threonyl‑tRNA synthetase (EC 6.1.1.3)/Type III restriction‑modification system methylation subunit (EC 2.1.1.72)
* ? 1 693781 =2 (0.020)15 (0.200) 14/392 3.6 94.3% coding (22/921 nt) unknown Glycosyl transferase, family 2
?1 = 693776 0 (0.000)coding (27/921 nt) unknown Glycosyl transferase, family 2
* ? 1 669993 =77 (0.920)6 (0.100)
+86 bp
6/258 4.2 20.7% intergenic (+26/‑156) unknown/unknown tRNA‑Pro‑TGG/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
?2 = 176511 0 (0.000)intergenic (‑277/–) unknown/– Transaldolase (EC 2.2.1.2)/–
* ? 1 = 131034892 (1.090)8 (0.140)
+76 bp
6/278 4.5 13.8% coding (213/645 nt) unknown Putative transposase InsK for insertion sequence element IS150
?3 20593 = 63 (0.650)coding (181/348 nt) unknown hypothetical protein
* ? 1 673420 =NA (NA)12 (0.150) 9/396 5.0 NA noncoding (1165/3023 nt) unknown LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
?1 673559 = NA (NA)noncoding (1304/3023 nt) unknown LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
* ? 1 623657 =65 (0.770)14 (0.170)
+G
10/428 5.0 30.2% intergenic (+144/+532) unknown/unknown Outer membrane protein A precursor/PTS system, N‑acetylglucosamine‑specific IIC component / PTS system, N‑acetylglucosamine‑specific IIB component (EC 2.7.1.193)
?1 1202140 = 0 (0.000)intergenic (+131/+464) unknown/unknown Na+/H+ antiporter/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
* ? 1 1299093 =NA (NA)19 (0.270)
+36 bp
7/358 5.2 57.3% intergenic (‑177/+395) unknown/unknown tRNA‑Asp‑GTC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
?1 = 1657605 17 (0.200)intergenic (‑147/+94) unknown/unknown Transposase, IS200 family/CTP synthase (EC 6.3.4.2)
* ? 2 = 15490896 (0.900)23 (0.230)
+ATTT
11/412 6.3 31.0% intergenic (‑271/+796) unknown/unknown Deoxyribose‑phosphate aldolase (EC 4.1.2.4)/hypothetical protein
?3 = 20679 6 (0.060)coding (95/348 nt) unknown hypothetical protein
* ? 1 1202140 =2 (0.020)9 (0.110)
+C
6/428 6.4 24.4% intergenic (+131/+464) unknown/unknown Na+/H+ antiporter/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
?1 = 1648073 54 (0.640)intergenic (+40/+88) unknown/unknown hypothetical protein/LSU ribosomal protein L17p