breseq  version 0.38.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 598 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1311,4010AC79.2% 174.7 / 91.6 179L45W (TTG→TGG) Xen14_04738hypothetical protein
*Xen14_1439150CT79.1% 2331.9 / inf 1379intergenic (‑609/–)Xen14_04759/–hypothetical protein/–
*Xen14_1261,9000AT78.9% 2876.4 / inf 1461R38R (CGA→CGT) Xen14_04726hypothetical protein
*Xen14_1262,0620TC78.2% 1392.3 / inf 855A92A (GCT→GCC) Xen14_04726hypothetical protein
*Xen14_1262,0630CA77.9% 1695.0 / inf 857L93I (CTT→ATT) Xen14_04726hypothetical protein
*Xen14_1311,4290GA77.9% 190.7 / 66.6 172L36L (CTG→TTG) Xen14_04738hypothetical protein
*Xen14_1311,4140AC77.6% 181.3 / 100.5 179Y41D (TAT→GAT) Xen14_04738hypothetical protein
*Xen14_1431550GA77.6% 1203.4 / inf 760A51V (GCG→GTG) Xen14_04759hypothetical protein
*Xen14_1262,0680TG77.5% 1827.0 / inf 863T94T (ACT→ACG) Xen14_04726hypothetical protein
*Xen14_1262,0590TG77.4% 1775.1 / inf 858A91A (GCT→GCG) Xen14_04726hypothetical protein
*Xen14_281320GC77.2% 428.4 / 132.5 203intergenic (–/‑453)–/gadC–/putative glutamate/gamma‑aminobutyrate antiporter
*Xen14_1262,0660AG77.1% 1377.3 / inf 862T94A (ACT→GCT) Xen14_04726hypothetical protein
*Xen14_1779,6270TC76.5% 892.9 / inf 1005S215G (AGC→GGC) ‡Xen14_02064IS4 family transposase ISEc13
*Xen14_1779,6250GA76.4% 888.1 / inf 1006S215S (AGC→AGT) ‡Xen14_02064IS4 family transposase ISEc13
*Xen14_1779,6280TG76.1% 854.3 / inf 1007T214T (ACA→ACC) Xen14_02064IS4 family transposase ISEc13
*Xen14_1779,6240TG75.9% 843.0 / inf 1007S216R (AGC→CGC) Xen14_02064IS4 family transposase ISEc13
*Xen14_1311,3760AG75.9% 118.3 / 69.6 162H53H (CAT→CAC) Xen14_04738hypothetical protein
*Xen14_1261,9870AG75.4% 1858.6 / inf 1247T67T (ACA→ACG) Xen14_04726hypothetical protein
*Xen14_1261,8550TG75.2% 1915.4 / inf 1378V23V (GTT→GTG) Xen14_04726hypothetical protein
*Xen14_3848,3370AG75.2% 709.4 / 283.7 596Y167H (TAC→CAC) Xen14_03355IS21 family transposase IS100kyp

Marginal new junction evidence (lowest skew 10 of 176 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Xen14_168 = 2982043 (1.210)84 (0.100) 57/296 0.1 3.9% coding (221/459 nt) Xen14_04774 IS200/IS605 family transposase IS200C
?Xen14_94 7510 = NA (NA)intergenic (‑130/–) Xen14_04561/– hypothetical protein/–
* ? Xen14_12 25 =963 (11.610)61 (0.050) 46/280 0.6 11.8% intergenic (–/+303) –/Xen14_01540 –/hypothetical protein
?Xen14_166 = 677 0 (0.000)intergenic (–/–) –/– –/–
* ? Xen14_166 = 3301284 (0.510)97 (0.070) 61/296 0.8 12.0% intergenic (–/–) –/– –/–
?Xen14_38 48182 = 133 (1.080)intergenic (+768/+108) Xen14_03354/Xen14_03355 hypothetical protein/IS21 family transposase IS100kyp
* ? Xen14_117 = 3027108 (1.210)4 (0.060)
+CCGGAGCCGGACTCCGGTTT
3/256 1.8 5.1% intergenic (‑290/–) Xen14_04703/– hypothetical protein/–
?Xen14_194 = 327 64 (0.880)intergenic (+18/–) Xen14_04786/– hypothetical protein/–
* ? Xen14_143 808 =1165 (1.470)46 (0.110)
+AC
29/292 1.9 3.8% intergenic (‑502/–) Xen14_04759/– hypothetical protein/–
?Xen14_94 = 75 NA (NA)intergenic (–/‑39) –/Xen14_04554 –/hypothetical protein
* ? Xen14_138 1232 =121 (0.860)43 (0.030) 32/296 1.9 2.9% intergenic (‑5/–) scrY_2/– Sucrose porin/–
?Xen14_166 103 = 2762 (1.110)intergenic (–/–) –/– –/–
* ? Xen14_105 = 4988348 (2.040)80 (0.550) 42/296 1.9 18.7% intergenic (+229/–) Xen14_04638/– IS3 family transposase ISCfr6/–
?Xen14_152 859 = NA (NA)intergenic (–/–) –/– –/–
* ? Xen14_138 1231 =121 (0.860)24 (0.020) 20/294 2.3 3.2% intergenic (‑4/–) scrY_2/– Sucrose porin/–
?Xen14_166 327 = 1345 (0.540)intergenic (–/–) –/– –/–
* ? Xen14_138 1232 =121 (0.860)97 (0.250) 48/296 2.4 16.7% intergenic (‑5/–) scrY_2/– Sucrose porin/–
?Xen14_202 276 = 848 (1.310)intergenic (–/–) –/– –/–
* ? Xen14_202 = 280859 (1.330)75 (0.200) 38/296 2.7 13.1% intergenic (–/–) –/– –/–
?Xen14_38 48182 = 133 (1.080)intergenic (+768/+108) Xen14_03354/Xen14_03355 hypothetical protein/IS21 family transposase IS100kyp