Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_3848,3370AG75.2% 709.4 / 283.7 596Y167H (TAC→CAC) Xen14_03355IS21 family transposase IS100kyp
Reads supporting (aligned to +/- strand):  ref base A (74/74);  new base G (242/206);  total (316/280)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 598 total aligned reads displayed.

ACGGATGAAAAGTGATCCACTTATATCTCCACCAACGACCCAATATTGATCCACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTGCTT  >  Xen14_38/48190‑48483
                                                                                                                                                   |                                                                                                                                                  
       aaaaGTGATCCACTTATATCTCCACCAACGACCCAATATTGATCCACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGAt                                                                                                                                           >  1:1198355/1‑150 (MQ=255)
        aaaGTGATCCACTTATATCTCCACCAACGACCCAATATTGATCCACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATa                                                                                                                                          >  1:2099304/1‑150 (MQ=255)
             gATCCACTTATATCTCCACCAACGACCCAATATTGATCCACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTc                                                                                                                                     >  1:1284017/1‑150 (MQ=255)
                ccACTTATATCTCCACCAACGACCCAATATTGATCCACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAg                                                                                                                                  <  1:144774/150‑1 (MQ=255)
                                               gATCCACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCg                                                                                                   >  1:1072822/1‑150 (MQ=255)
                                                   cACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACg                                                                                               >  1:1253380/1‑150 (MQ=255)
                                                      cGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTAAGTCGATAACTTTCTCCTTTGATTTGAAGGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCGGAGGTCAGGGCTGCATCACCGGCGAACGttg                                                                                            <  1:69727/150‑2 (MQ=17)
                                                                       gCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAAc                                                                           >  1:4040041/1‑150 (MQ=255)
                                                                                   aaCTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGAt                                                               <  1:1840474/150‑1 (MQ=255)
                                                                                     cTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATATGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGt                                                             >  1:683611/1‑150 (MQ=255)
                                                                                           cTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGAt                                                       <  1:3046653/150‑1 (MQ=255)
                                                                                                gTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTg                                                  >  1:1008239/1‑150 (MQ=255)
                                                                                                     tATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGAAAAATTGGATGTCAGGATCATTGCGctc                                             >  1:3561529/1‑150 (MQ=255)
                                                                                                        cTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCttt                                          >  1:2942963/1‑150 (MQ=255)
                                                                                                           aGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCTTTTCg                                       <  1:2903175/150‑1 (MQ=255)
                                                                                                             tCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCTTTTCGTa                                     <  1:4158446/150‑1 (MQ=255)
                                                                                                                    cTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACACATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTa                              >  1:3213395/1‑150 (MQ=255)
                                                                                                                    cTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTa                              >  1:243604/1‑150 (MQ=255)
                                                                                                                           cTTTGATTTGAACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCGGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGa                       >  1:3137207/1‑150 (MQ=11)
                                                                                                                           cTTTGATTTGAACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGa                       >  1:1476849/1‑150 (MQ=9)
                                                                                                                           cTTTGATTTGAACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGa                       >  1:3244269/1‑150 (MQ=9)
                                                                                                                           cTTTGATTTGAACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGa                       >  1:456869/1‑150 (MQ=9)
                                                                                                                           cTTTGATTTGAACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGa                       >  1:132698/1‑150 (MQ=9)
                                                                                                                            tttgatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCTCTCTTTTCGTAACGTTTAGCAATGAc                      >  1:186004/1‑150 (MQ=11)
                                                                                                                            tttgatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGAc                      >  1:1396499/1‑150 (MQ=255)
                                                                                                                             ttgatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGAcc                     <  1:61904/150‑1 (MQ=255)
                                                                                                                             ttgatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGAcc                     <  1:899156/150‑1 (MQ=255)
                                                                                                                             gtgatttgaACGACGTGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCTAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGAcc                     <  1:3926874/149‑1 (MQ=11)
                                                                                                                             atcgtcgtgaTGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGAcc                     <  1:265338/141‑1 (MQ=11)
                                                                                                                              tgatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCt                    <  1:2573024/150‑1 (MQ=255)
                                                                                                                               gatttgaATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCTTTTCg                                       >  1:1366217/1‑130 (MQ=255)
                                                                                                                               gatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTg                   <  1:3399408/150‑1 (MQ=255)
                                                                                                                               gatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTg                   >  1:3977522/1‑150 (MQ=255)
                                                                                                                               gatttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTg                   <  1:182046/150‑1 (MQ=255)
                                                                                                                                atttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAATGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTgg                  <  1:91688/150‑1 (MQ=11)
                                                                                                                                atttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCGGAGGTCAGTGCTGCATCACTGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTgg                  >  1:302124/1‑150 (MQ=11)
                                                                                                                                 tttgaATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACCTGga                 >  1:26177/1‑150 (MQ=255)
                                                                                                                                 tttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGga                 <  1:2357160/150‑1 (MQ=255)
                                                                                                                                 tttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGga                 <  1:1786902/150‑1 (MQ=255)
                                                                                                                                 tttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGga                 >  1:3659816/1‑150 (MQ=255)
                                                                                                                                  ttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACCTGgaa                <  1:1523675/150‑1 (MQ=11)
                                                                                                                                  ttgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaa                <  1:2324573/150‑1 (MQ=255)
                                                                                                                                  ctgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaa                >  1:1953461/2‑150 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACTGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACTTGgaag               <  1:551329/150‑1 (MQ=11)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTACCGTTTAGCGATGACCTGgaag               >  1:3856326/1‑150 (MQ=11)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               >  1:816385/1‑150 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               <  1:1093400/150‑1 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               >  1:3628606/1‑150 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               >  1:3176802/1‑150 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               >  1:2774557/1‑150 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               <  1:2391194/150‑1 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               >  1:754126/1‑150 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               >  1:2981986/1‑150 (MQ=255)
                                                                                                                                   tgaACGACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCACGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaag               <  1:2815520/150‑1 (MQ=11)
                                                                                                                                    gaacgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaaga              >  1:3059907/5‑150 (MQ=255)
                                                                                                                                    gaacgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaaga              >  1:3729461/5‑150 (MQ=255)
                                                                                                                                    gaacgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaaga              <  1:2068587/146‑1 (MQ=255)
                                                                                                                                    gaacgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACAGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaaga              >  1:776281/5‑150 (MQ=11)
                                                                                                                                    gaacgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAAAGTTTGATCCCACTGCCCGAACGGCAGATTGGCTGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGgaaga              >  1:2831598/5‑150 (MQ=11)
                                                                                                                                     aacgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGACCGTATAATCCCACTGCCCAAACGGCAGATTGGATGTCGGGATCATCGCGCTCTTTTCGTAGCGTTTAGCGATGACCTGgaggaa             >  1:2682287/4‑150 (MQ=11)
                                                                                                                                     aaTGACATGTGAGTAGTGTAAGATACGGTCCCGCATCGCTGAGGTCAGGGCGGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATCGCGCTCTTTTCGTAACGTTTAGCGATGACCTGgaagta             >  1:2190920/1‑148 (MQ=17)
                                                                                                                                      acgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCTAACGGCAGATTGGATGTCAGGATCATTGCGGTCTTTTCGTAACTTTTAGCAATGACCCGGAAAAAc            >  1:3814607/3‑150 (MQ=11)
                                                                                                                                      acgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAAc            <  1:2853598/148‑1 (MQ=255)
                                                                                                                                      acgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAAc            <  1:2291847/148‑1 (MQ=255)
                                                                                                                                      acgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAAc            >  1:2046185/3‑150 (MQ=255)
                                                                                                                                      acgACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTCTCGTAACGTTTAGCAATGACCTGGAAGAAc            <  1:1143571/148‑1 (MQ=255)
                                                                                                                                        gACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACCGGGAAGAACAg          <  1:4107687/150‑1 (MQ=11)
                                                                                                                                        gACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAg          <  1:1973246/150‑1 (MQ=255)
                                                                                                                                        gACATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAg          >  1:300096/1‑150 (MQ=255)
                                                                                                                                         aCATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGt         >  1:3590978/1‑150 (MQ=255)
                                                                                                                                         aCATGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGt         <  1:1640566/150‑1 (MQ=255)
                                                                                                                                           aTGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGttt       >  1:2488493/1‑150 (MQ=255)
                                                                                                                                           aTGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGttt       <  1:2118110/150‑1 (MQ=255)
                                                                                                                                           aTGTGAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAATGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGttt       <  1:4219967/150‑1 (MQ=255)
                                                                                                                                           aTGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACCTGGAAGTACAgctt       <  1:53023/150‑4 (MQ=17)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCCGCATCGCTGAGGTCAGTGCTGCATCACCGGCAAACGTTTGATCCCACTACCCGAACGGCCGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGtttt      >  1:2598448/1‑150 (MQ=17)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCTGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGtttt      >  1:3958696/1‑150 (MQ=255)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTCGCGATGACCTGGAAGAACAGcttt      >  1:3530978/1‑150 (MQ=11)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACCTGGAAGAACAGcttt      >  1:3289934/1‑150 (MQ=14)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGtttt      <  1:1698090/150‑1 (MQ=255)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGtttt      >  1:2217641/1‑150 (MQ=255)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGtttt      <  1:2516369/150‑1 (MQ=255)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGtttt      >  1:861697/1‑150 (MQ=255)
                                                                                                                                            tgtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACAGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGtttt      >  1:400895/1‑150 (MQ=255)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCGGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTg     <  1:1496207/150‑1 (MQ=255)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTTCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGttgtg     <  1:3313294/150‑4 (MQ=14)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCGTTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTg     <  1:602833/150‑1 (MQ=255)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAATGTTTAGCAATGACCTGGAAGAACAGTTTTg     >  1:2153001/1‑150 (MQ=255)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTg     >  1:1594861/1‑150 (MQ=255)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTg     <  1:1687278/150‑1 (MQ=255)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTg     <  1:959691/150‑1 (MQ=255)
                                                                                                                                             gtgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTg     <  1:3434345/150‑1 (MQ=255)
                                                                                                                                              tgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGGCCTGGAAGAACAGTTTTGc    <  1:333461/150‑1 (MQ=255)
                                                                                                                                              tgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTGc    <  1:668146/150‑1 (MQ=255)
                                                                                                                                              tgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTGc    <  1:4134574/150‑1 (MQ=255)
                                                                                                                                              tgAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTGc    <  1:1437035/150‑1 (MQ=255)
                                                                                                                                               gAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACCTGGAAGAACAGCTTTGct   <  1:3487275/150‑1 (MQ=14)
                                                                                                                                               gAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTGct   <  1:1730939/150‑1 (MQ=255)
                                                                                                                                               gAGTGGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGTGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGCTTTGct   >  1:2240860/1‑150 (MQ=255)
                                                                                                                                               gAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGACAAATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCGATGACCTGGAAGTACAGCTTTGct   <  1:255749/150‑1 (MQ=17)
                                                                                                                                                   |                                                                                                                                                  
ACGGATGAAAAGTGATCCACTTATATCTCCACCAACGACCCAATATTGATCCACCGTTTTACTCAGGATTAGCTTCTGCTATAAACTTGGCCTTTCGTTTCTATCTGAGTCGATAGCTTTCTCCTTTGATTTGAATGACATGTGAGTAGTGTAAGATACGGTCCAGCATCGCTGAGGTCAGGGCTGCATCACCGGCGAACGTTTGATCCCACTGCCCGAACGGCAGATTGGATGTCAGGATCATTGCGCTCTTTTCGTAACGTTTAGCAATGACCTGGAAGAACAGTTTTGCTT  >  Xen14_38/48190‑48483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑