breseq  version 0.35.4  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 183 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,481,4080AT76.5% 107.6 / 32.7 51intergenic (+59/‑950)unknown/unknownZona occludens toxin‑like phage protein/Bacterial non‑heme ferritin (EC 1.16.3.2)
*11,481,4000TC72.2% 86.7 / 32.9 54intergenic (+51/‑958)unknown/unknownZona occludens toxin‑like phage protein/Bacterial non‑heme ferritin (EC 1.16.3.2)
*11,481,4020GT72.2% 89.0 / 44.4 54intergenic (+53/‑956)unknown/unknownZona occludens toxin‑like phage protein/Bacterial non‑heme ferritin (EC 1.16.3.2)
*11,481,3960AT67.8% 86.9 / 59.7 59intergenic (+47/‑962)unknown/unknownZona occludens toxin‑like phage protein/Bacterial non‑heme ferritin (EC 1.16.3.2)
*11,645,3850CG61.8% 160.3 / 193.0 144A312A (GCC→GCG) unknownhypothetical protein
*11,476,3310AC57.4% 28.6 / 67.2 54T1108P (ACC→CCC) unknownChromosome partition protein MukB
*11,645,3540CT54.6% 40.3 / 174.2 142A302V (GCG→GTG) unknownhypothetical protein
*1960,2191.C52.0% 81.5 / 93.1 50intergenic (+222/‑181)unknown/unknownMagnesium and cobalt efflux protein CorC/Apolipoprotein N‑acyltransferase / Copper homeostasis protein CutE
*11,645,4310CA50.0% 18.8 / 204.9 138Q328K (CAA→AAA) unknownhypothetical protein
*11,645,4290TC49.6% 6.2 / 172.9 135V327A (GTC→GCC) ‡unknownhypothetical protein
*11,748,6430AC49.2% 70.4 / 27.1 65L54V (TTG→GTG) unknownN‑acetylmannosamine‑6‑phosphate 2‑epimerase (EC 5.1.3.9)
*11,645,4300CA48.9% 19.1 / 210.3 138V327V (GTC→GTA) ‡unknownhypothetical protein
*11,927,4510CG44.8% 21.5 / 12.6 30intergenic (+69/‑66)unknown/unknownHydrogenase‑4 component I/Hydrogenase‑4 maturation protease
*1960,2111.C44.0% 6.6 / 102.9 50intergenic (+214/‑189)unknown/unknownMagnesium and cobalt efflux protein CorC/Apolipoprotein N‑acyltransferase / Copper homeostasis protein CutE
*1789,3400GA43.8% 44.5 / 22.3 48intergenic (‑41/‑98)unknown/unknownhypothetical protein/Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)
*11,669,8690AC43.3% 92.2 / 14.9 68V80G (GTC→GGC) unknown4‑hydroxy‑2‑oxoglutarate aldolase (EC 4.1.3.16) @ 2‑dehydro‑3‑deoxyphosphogluconate aldolase (EC 4.1.2.14)
*11,072,1760AC42.7% 122.2 / 11.9 75V366G (GTC→GGC) unknownPhosphoglucosamine mutase (EC 5.4.2.10)
*11,344,4930TG41.5% 105.9 / 10.7 65T88P (ACC→CCC) unknownTyrosyl‑tRNA synthetase (EC 6.1.1.1)
*11,645,3820AG41.5% 82.9 / 144.8 148E311E (GAA→GAG) unknownhypothetical protein
*11,988,4320TA41.0% 119.0 / 37.0 78N388K (AAT→AAA) unknownUncharacterized protease YegQ

Marginal new junction evidence (lowest skew 10 of 65 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? 1 = 264889NA (NA)12 (0.150) 9/396 4.7 NA noncoding (1287/3023 nt) unknown LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
?1 = 264994 NA (NA)noncoding (1182/3023 nt) unknown LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
* ? 1 = 86574789 (1.020)10 (0.120) 9/406 4.8 10.9% coding (642/1923 nt) unknown tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
?1 = 865993 80 (0.970)coding (888/1923 nt) unknown tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
* ? 1 = 5609NA (NA)8 (0.090) 7/420 5.5 NA coding (10/762 nt) unknown Mobile element protein
?1 = 5819 NA (NA)coding (220/762 nt) unknown Mobile element protein
* ? 1 = 92811461 (0.700)3 (0.050)
+72 bp
3/286 5.8 12.9% intergenic (‑368/+159) unknown/unknown Soluble lytic murein transglycosylase (EC 4.2.2.n1)/YciL protein
?1 960294 = 0 (0.000)intergenic (+297/‑106) unknown/unknown Magnesium and cobalt efflux protein CorC/Apolipoprotein N‑acyltransferase / Copper homeostasis protein CutE
* ? 1 1248990 =101 (1.150)6 (0.070) 5/412 6.2 5.7% coding (734/1953 nt) unknown Electron transport complex protein RnfC
?1 1249377 = 100 (1.190)coding (1121/1953 nt) unknown Electron transport complex protein RnfC
* ? 1 = 877207115 (1.310)5 (0.060) 5/418 6.2 4.4% intergenic (+260/‑4) unknown/unknown Carbon starvation protein A/Transcriptional regulator MraZ
?1 = 877341 106 (1.240)coding (131/459 nt) unknown Transcriptional regulator MraZ
* ? 1 732132 =79 (0.900)6 (0.070) 5/410 6.2 6.1% coding (39/1140 nt) unknown A/G‑specific adenine glycosylase (EC 3.2.2.‑)
?1 732475 = 111 (1.330)coding (382/1140 nt) unknown A/G‑specific adenine glycosylase (EC 3.2.2.‑)
* ? 1 = 124632889 (1.020)5 (0.060) 5/422 6.3 5.3% coding (322/888 nt) unknown Cell division inhibitor Slr1223 (YfcH in EC), contains epimerase/dehydratase and DUF1731 domains
?1 = 1246580 92 (1.070)coding (574/888 nt) unknown Cell division inhibitor Slr1223 (YfcH in EC), contains epimerase/dehydratase and DUF1731 domains
* ? 1 = 135683076 (0.870)4 (0.050) 4/386 6.4 5.3% intergenic (‑34/+162) unknown/unknown Autoinducer 2 (AI‑2) ABC transporter, substrate‑binding protein/Excinuclease ABC subunit C
?1 = 1356880 74 (0.940)intergenic (‑84/+112) unknown/unknown Autoinducer 2 (AI‑2) ABC transporter, substrate‑binding protein/Excinuclease ABC subunit C
* ? 1 264873 =NA (NA)6 (0.070) 4/396 6.5 NA noncoding (1303/3023 nt) unknown LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
?1 265012 = NA (NA)noncoding (1164/3023 nt) unknown LSU rRNA ## 23S rRNA, large subunit ribosomal RNA