breseq  version 0.35.0  revision fe39cd8f5880
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 312 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NODE_934310GC74.5% 1263.2 / inf 742intergenic (–/–)–/––/–
*NODE_921240AG73.6% 764.9 / inf 639intergenic (–/–)–/––/–
*NODE_921380TG71.9% 651.4 / inf 652intergenic (–/–)–/––/–
*NODE_934890TC71.9% 1652.3 / inf 902intergenic (–/–)–/––/–
*NODE_923160TG71.9% 1383.4 / inf 768intergenic (–/–)–/––/–
*NODE_921320CT71.8% 588.0 / inf 651intergenic (–/–)–/––/–
*NODE_921360GA71.8% 583.0 / inf 653intergenic (–/–)–/––/–
*NODE_934830CT71.7% 1391.6 / inf 879intergenic (–/–)–/––/–
*NODE_921350TC71.7% 686.1 / inf 654intergenic (–/–)–/––/–
*NODE_92780CT71.2% 216.4 / inf 396intergenic (–/–)–/––/–
*NODE_923120GC71.1% 1103.5 / inf 708intergenic (–/–)–/––/–
*NODE_921560CG71.0% 653.5 / inf 652intergenic (–/–)–/––/–
*NODE_923090CT71.0% 956.0 / inf 708intergenic (–/–)–/––/–
*NODE_923110GA70.9% 927.3 / inf 708intergenic (–/–)–/––/–
*NODE_921860TC69.9% 735.4 / inf 615intergenic (–/–)–/––/–
*NODE_921590CG69.4% 559.9 / inf 612intergenic (–/–)–/––/–
*NODE_921440CT69.2% 441.5 / inf 613intergenic (–/–)–/––/–
*NODE_921630AG69.0% 594.5 / inf 617intergenic (–/–)–/––/–
*NODE_921470TG68.9% 511.3 / inf 614intergenic (–/–)–/––/–
*NODE_921450AG68.8% 544.7 / inf 613intergenic (–/–)–/––/–

Marginal new junction evidence (lowest skew 10 of 223 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NODE_53 = 108493 (0.790)19 (0.350)
+136 bp
13/26 3.2 28.7% intergenic (–/+165) –/EcoliAnna_04105 –/NAD(P)H‑flavin reductase
?NODE_53 152 = 590 (0.940)intergenic (–/+121) –/EcoliAnna_04105 –/NAD(P)H‑flavin reductase
* ? NODE_2 243922 =222 (1.020)145 (0.510)
+49 bp
62/200 3.2 66.1% intergenic (+15/+219) EcoliAnna_00630/EcoliAnna_00631 putative acyl‑CoA thioester hydrolase/hydratase
?NODE_61 = 7785 0 (0.000)intergenic (+152/–) fadA/– acetyl‑CoA C‑acyltransferase FadA/–
* ? NODE_18 86488 =NA (NA)167 (1.040)
+60 bp
64/178 3.2 NA intergenic (‑280/+1) yqaB/EcoliAnna_02742 fructose‑1‑phosphate/6‑phosphogluconate phosphatase/tRNA‑Arg
?NODE_6 = 77 NA (NA)intergenic (‑1/+3) EcoliAnna_01179/EcoliAnna_01180 tRNA‑Arg/tRNA‑Ser
* ? NODE_4 77345 =137 (0.660)104 (0.560) 81/266 3.3 46.2% coding (38/537 nt) EcoliAnna_00891 integrase
?NODE_4 79174 = 120 (0.640)coding (289/630 nt) EcoliAnna_00894 integrase
* ? NODE_18 = 190234 (0.880)218 (0.670) 79/218 3.4 71.8% intergenic (–/+115) –/clpB –/ATP‑dependent chaperone ClpB
?NODE_61 41 = 0 (0.000)intergenic (–/+48) –/hemG –/menaquinone‑dependent protoporphyrinogen IX dehydrogenase
* ? NODE_17 = 67973304 (1.020)44 (0.600)
+112 bp
25/74 3.4 36.8% intergenic (‑80/+78) nrdG/nrdD anaerobic ribonucleoside‑triphosphate reductase‑activating protein/anaerobic ribonucleoside‑triphosphate reductase
?NODE_17 67993 = 306 (1.030)intergenic (‑100/+58) nrdG/nrdD anaerobic ribonucleoside‑triphosphate reductase‑activating protein/anaerobic ribonucleoside‑triphosphate reductase
* ? NODE_55 = 145530 (0.000)99 (0.060) 70/252 3.6 100% intergenic (‑463/–) narP/– nitrate/nitrite response regulator protein NarP/–
?NODE_83 24 = 0 (0.000)coding (10/981 nt) EcoliAnna_04298 IS5‑like element IS5 family transposase
* ? NODE_1 74136 =194 (0.950)97 (0.500)
+33 bp
63/232 3.7 38.6% noncoding (1/76 nt) EcoliAnna_00080 tRNA‑Gly
?NODE_17 = 189 203 (0.680)intergenic (+2/+268) EcoliAnna_02550/queG tRNA‑Gly/tRNA epoxyqueuosine(34) reductase QueG
* ? NODE_66 1 =0 (0.000)143 (0.090) 93/252 3.8 100% intergenic (–/‑86) –/EcoliAnna_04258 –/hypothetical protein
?NODE_83 24 = 0 (0.000)coding (10/981 nt) EcoliAnna_04298 IS5‑like element IS5 family transposase
* ? NODE_10 = 1332300 (0.000)166 (0.100) 97/258 4.1 100% intergenic (‑4/–) EcoliAnna_01911/– N‑acetyltransferase/–
?NODE_83 21 = 0 (0.000)coding (7/981 nt) EcoliAnna_04298 IS5‑like element IS5 family transposase