BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_92	124	0	A	G	73.6%	764.9 / inf	639	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (81/87); new base G (236/233); total (318/321)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 640 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGC  >  NODE_92/1‑273

tggacacccactggcACTATGACGAAGCAGACCGCCACACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATg                                                                                                                                           <  2:429570/136‑1 (MQ=21)

    cacccactggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTa                                                                                                                                       <  1:2612735/140‑1 (MQ=21)

        cactggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGat                                                                                                                                   <  2:1004464/144‑2 (MQ=18)

        cactggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGat                                                                                                                                   <  2:2678764/144‑2 (MQ=18)

        cactggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGat                                                                                                                                   >  1:3900187/7‑149 (MQ=18)

         actggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACa                                                                                                                                  >  2:227597/6‑150 (MQ=255)

         actggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatg                                                                                                                                  >  2:3454978/6‑148 (MQ=18)

            ggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgaga                                                                                                                               >  2:1641003/3‑145 (MQ=21)

             gcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaa                                                                                                                              <  2:4056190/149‑7 (MQ=21)

              cACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaa                                                                                                                             <  1:2412815/150‑8 (MQ=21)

               aCTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaag                                                                                                                            <  1:1566018/150‑9 (MQ=21)

               aCTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaag                                                                                                                            <  2:674785/150‑9 (MQ=21)

                 tATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGgc                                                                                                                          <  1:3806584/150‑1 (MQ=255)

                  aTGACGAAGCAGACCGCCTCACGCACCGCACTGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaaggcc                                                                                                                         <  1:537506/150‑12 (MQ=21)

                  aTGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaaggcc                                                                                                                         <  1:1837008/150‑12 (MQ=21)

                  aTGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaaggcc                                                                                                                         <  2:2224125/150‑12 (MQ=21)

                   tGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGgccg                                                                                                                        >  2:1197703/1‑150 (MQ=21)

                    gACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGGGAAAGgccgg                                                                                                                       >  2:851536/1‑149 (MQ=21)

                    gACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGgccgg                                                                                                                       <  1:1003681/150‑2 (MQ=21)

                       gAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTAAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtg                                                                                                                    <  1:3684069/150‑2 (MQ=21)

                        aaGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtga                                                                                                                   >  2:3408095/1‑148 (MQ=21)

                        aaGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtga                                                                                                                   <  2:2623952/150‑3 (MQ=21)

                           cAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtgaccg                                                                                                                <  2:4114539/150‑6 (MQ=21)

                            aGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtgaccgg                                                                                                               >  2:78297/1‑144 (MQ=21)

                             gACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGTCGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGt                                                                                                              >  1:4029004/1‑150 (MQ=255)

                             gACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtgaccggt                                                                                                              >  2:3636305/1‑143 (MQ=21)

                              aCCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtgaccggtg                                                                                                             <  1:2530187/150‑9 (MQ=21)

                                cGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGag                                                                                                           <  2:1358970/150‑2 (MQ=21)

                                 gCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAac                                                                                                          <  2:1089047/150‑1 (MQ=255)

                                 gCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAac                                                                                                          <  2:1826172/150‑1 (MQ=255)

                                  ccTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGagcg                                                                                                         >  1:2797902/1‑147 (MQ=18)

                                    tCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGagcgtc                                                                                                       >  1:2163076/1‑145 (MQ=18)

                                        gcaccgcaccGTGAAGGGTGAAACCGCAGGGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGagcgtcagac                                                                                                   <  1:2096448/150‑10 (MQ=18)

                                                ccgTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatc                                                                                           <  2:2045836/150‑1 (MQ=18)

                                                  gTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatcac                                                                                         >  1:3991009/1‑149 (MQ=18)

                                                  gTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatcac                                                                                         >  2:2163302/1‑149 (MQ=18)

                                                  gTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatcac                                                                                         <  2:2481999/150‑2 (MQ=18)

                                                  gTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatcac                                                                                         <  1:26773/150‑2 (MQ=18)

                                                   tGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCatcatc                                                                                        >  2:1911493/1‑150 (MQ=255)

                                                    gAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATcc                                                                                       <  2:498844/150‑1 (MQ=255)

                                                    gAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCAccc                                                                                       <  2:2809679/150‑1 (MQ=18)

                                                          tGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGGGCGTCAGACGGTGCATCACCCGCAGAc                                                                                 >  1:2042193/1‑150 (MQ=16)

                                                           gAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACg                                                                                <  2:3585875/150‑1 (MQ=18)

                                                            aaaCCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACgg                                                                               <  1:1054621/150‑2 (MQ=12)

                                                            aaaCCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACgg                                                                               >  1:661506/1‑149 (MQ=12)

                                                              aCCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAAt                                                                             <  2:2911002/150‑1 (MQ=255)

                                                              aCCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAAt                                                                             <  2:3115164/150‑1 (MQ=255)

                                                               ccGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGAGAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGGCGGCTGACCGGGGAGCGTCAGACGGTGCATCACCCGCAGACggaag                                                                            >  2:2960667/1‑146 (MQ=255)

                                                                  cAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACggaagcac                                                                         <  2:3999742/150‑8 (MQ=12)

                                                                  cAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACggaagcac                                                                         >  1:3334274/1‑143 (MQ=12)

                                                                  cAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAAc                                                                         <  2:1507418/150‑1 (MQ=255)

                                                                   agagCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACt                                                                        <  2:2115259/150‑1 (MQ=255)

                                                                      gCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGct                                                                     <  2:3963906/150‑1 (MQ=255)

                                                                       cGGTGGCAGAATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGctc                                                                    <  1:459567/150‑1 (MQ=12)

                                                                        ggTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAGAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGctct                                                                   >  1:849555/1‑150 (MQ=12)

                                                                        ggTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGctct                                                                   <  1:913740/150‑1 (MQ=12)

                                                                            gCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGgca                                                               <  1:2350795/150‑1 (MQ=255)

                                                                             cAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGgcag                                                              >  1:1380443/1‑150 (MQ=12)

                                                                              aGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTTgcagc                                                             >  1:2739559/1‑150 (MQ=255)

                                                                               gTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGgcagca                                                            >  2:2362990/1‑150 (MQ=12)

                                                                                  tGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATga                                                         >  2:3198233/1‑150 (MQ=12)

                                                                                     cGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGAc                                                      >  1:113267/1‑150 (MQ=9)

                                                                                      gAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCAGTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGAcc                                                     <  1:1466783/150‑1 (MQ=9)

                                                                                      gAACGCGGCTGGCTGCCAGACATCAGCCAGATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGAGAGTAAAGGCCACCGCGCCAGTGAACACCTGACGGTGCACCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGAcc                                                     <  1:1287305/150‑1 (MQ=255)

                                                                                         cgtggctggctGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGa                                                  <  1:1660632/147‑1 (MQ=9)

                                                                                          gcgGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGAc                                                 >  1:3611964/1‑150 (MQ=255)

                                                                                            ggctggcaGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACAt                                               <  1:1927087/150‑1 (MQ=255)

                                                                                               tggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCg                                            >  1:2249461/1‑150 (MQ=12)

                                                                                               tggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCg                                            <  1:1729492/150‑1 (MQ=255)

                                                                                                ggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGt                                           <  1:3168094/150‑1 (MQ=255)

                                                                                                ggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGt                                           >  1:3279995/1‑150 (MQ=255)

                                                                                                 gctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTa                                          >  1:2902858/1‑150 (MQ=255)

                                                                                                  ctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGGCATGCGTAc                                         >  1:3044593/1‑150 (MQ=255)

                                                                                                  ctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTAc                                         <  1:879225/150‑1 (MQ=255)

                                                                                                   tgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACa                                        >  2:1742143/1‑150 (MQ=255)

                                                                                                     acagacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGGGCATCACCCGCAGACGGAAGCACTGCTCTGCCAGCATGAGACCAGACAAGCGTACAAc                                      >  2:372001/1‑150 (MQ=255)

                                                                                                       agacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGc                                    <  1:122783/150‑1 (MQ=255)

                                                                                                        gacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGcg                                   >  2:3910511/1‑149 (MQ=255)

                                                                                                            tCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAgg                               <  2:3295677/150‑1 (MQ=255)

                                                                                                            tCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAgg                               <  2:1670912/150‑1 (MQ=255)

                                                                                                              aGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAgggg                             <  2:3767731/150‑2 (MQ=255)

                                                                                                                ccATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCt                           <  2:3859206/150‑1 (MQ=255)

                                                                                                                ccATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCt                           >  2:592499/1‑150 (MQ=255)

                                                                                                                ccATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCt                           <  1:1092835/150‑1 (MQ=255)

                                                                                                                 cATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTg                          <  2:2736897/150‑1 (MQ=255)

                                                                                                                  atatCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGTCCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTgg                         >  1:3501979/1‑150 (MQ=255)

                                                                                                                  atatCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGCGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTgg                         >  2:887628/1‑150 (MQ=255)

                                                                                                                  atatCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTgg                         >  2:2575308/1‑150 (MQ=255)

                                                                                                                   tatCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGc                        <  1:3747263/150‑1 (MQ=255)

                                                                                                                       aGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAAc                    <  2:1247758/150‑1 (MQ=255)

                                                                                                                         cGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCg                  >  1:2207916/1‑150 (MQ=255)

                                                                                                                          gAAGGGCACCGGGTGGCGGTGCATTTCAGGTATGATGTGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGTCGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGc                 >  1:4112415/1‑150 (MQ=255)

                                                                                                                           aaGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCt                >  1:3417346/1‑150 (MQ=255)

                                                                                                                            aGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACATCGCGCAGGGGCTGGCGAACCGCTg               <  1:2994792/150‑1 (MQ=255)

                                                                                                                             gggCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGt              >  2:1965289/1‑150 (MQ=255)

                                                                                                                                 aCCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATAc          >  2:1438594/1‑150 (MQ=255)

                                                                                                                                   cGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCg        <  2:460912/150‑1 (MQ=255)

                                                                                                                                     ggTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGa      >  1:765616/1‑150 (MQ=255)

                                                                                                                                      gTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGAc     >  2:301948/1‑150 (MQ=255)

                                                                                                                                         aCGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGc  <  2:2411098/150‑1 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGC  >  NODE_92/1‑273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`