BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_92	311	0	G	A	70.9%	927.3 / inf	708	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (105/99); new base A (246/251); total (353/355)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-12
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 726 total aligned reads displayed.

CAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCTGAACAGCC  >  NODE_92/162‑460

    gAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCCTGGAATGGCTGACCTACGGCAGCGgctggctg                                                                                                                                                   >  1:2824945/1‑150 (MQ=255)

      acacCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTc                                                                                                                                                 <  2:41204/150‑1 (MQ=255)

           agACGGTGCATCCCCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTggcaggca                                                                                                                                            <  2:1454393/149‑1 (MQ=255)

               ggTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGaa                                                                                                                                        >  2:1951458/1‑150 (MQ=21)

                 tGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAAc                                                                                                                                      <  1:1911493/150‑1 (MQ=255)

                 tGCACCACCCGCAGACGGATGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAAc                                                                                                                                      <  1:1669248/150‑1 (MQ=21)

                  gCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACt                                                                                                                                     <  2:3936441/150‑1 (MQ=255)

                    atcatcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCg                                                                                                                                   >  2:3451296/1‑150 (MQ=255)

                     tcaccCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACGTGGCAGGCATGAAACTCgg                                                                                                                                  >  2:3415316/1‑150 (MQ=255)

                      cacccGCAGACGGAAGCACTGGTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGc                                                                                                                                 >  1:227047/4‑150 (MQ=255)

                         ccGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGac                                                                                                                              >  2:2646200/1‑150 (MQ=255)

                         ccGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGac                                                                                                                              >  1:2519094/1‑150 (MQ=255)

                           gCAGACGGAAGCACTGCACTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGacac                                                                                                                            <  1:1857634/150‑1 (MQ=255)

                            cAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGacaca                                                                                                                           >  2:3747745/1‑150 (MQ=255)

                                 ggaagcaCTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGGATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAACGGTTTCCTGGCAGGCATCAAACTCGGCCACACACCGCt                                                                                                                      >  1:3239344/7‑150 (MQ=255)

                                  gaagcaCTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCtg                                                                                                                     <  1:3454978/145‑1 (MQ=255)

                                    tgcaCTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCtggt                                                                                                                   >  2:3670702/4‑150 (MQ=255)

                                     gcaCTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCtggtg                                                                                                                  <  2:2768541/148‑1 (MQ=25)

                                     gcaCTGCACTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCtggtg                                                                                                                  <  1:364159/148‑1 (MQ=25)

                                         tGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGt                                                                                                              >  2:753295/1‑150 (MQ=255)

                                         tGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGt                                                                                                              <  1:1155770/150‑1 (MQ=255)

                                           ctctGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTac                                                                                                            >  2:130643/1‑150 (MQ=25)

                                             ctGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTacac                                                                                                          <  2:3389756/150‑1 (MQ=255)

                                              tGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACAcc                                                                                                         >  2:3253871/1‑150 (MQ=255)

                                                 cagcaTGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCcgc                                                                                                      >  2:2599920/1‑150 (MQ=255)

                                                    caTGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGAc                                                                                                   >  1:2960031/1‑150 (MQ=255)

                                                     aTGAGACCAGACATGCGTACCACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGAcc                                                                                                  <  2:2514026/150‑1 (MQ=25)

                                                     aTGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGAcc                                                                                                  >  1:2639778/1‑150 (MQ=255)

                                                      tGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCg                                                                                                 >  1:4143838/1‑150 (MQ=255)

                                                       gagaCCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGc                                                                                                >  2:389118/1‑150 (MQ=25)

                                                       gagaCCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGc                                                                                                >  1:3538615/1‑150 (MQ=25)

                                                        agaCCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGcc                                                                                               >  2:2236836/1‑150 (MQ=255)

                                                         gaCCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCt                                                                                              >  2:1640040/1‑150 (MQ=255)

                                                              gACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGTCCTACGGCAGCGGTTACCTGGCAGGCATGTAACTCGGCGACACACCGCTGGAGGAGTACACCCGCGACCGCCTGCAcc                                                                                         >  1:3899811/1‑150 (MQ=255)

                                                              gACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCAcc                                                                                         <  1:3794844/150‑1 (MQ=255)

                                                               aCATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCg                                                                                        >  2:314282/1‑150 (MQ=25)

                                                                cATGCGTCCAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCgg                                                                                       <  1:1742145/150‑1 (MQ=255)

                                                                    cGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGgaa                                                                                   >  1:4022868/1‑150 (MQ=255)

                                                                    cGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGaaa                                                                                   >  2:1856914/1‑150 (MQ=255)

                                                                     gTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGCCAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAAc                                                                                  <  1:1959988/150‑1 (MQ=25)

                                                                     gTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAAc                                                                                  <  2:2308515/150‑1 (MQ=255)

                                                                      tACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCCACCGCCTGCACCGGGACACg                                                                                 >  2:3300333/1‑150 (MQ=255)

                                                                       aCAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGc                                                                                >  1:875016/1‑150 (MQ=25)

                                                                          aCGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTgc                                                                             <  1:2744467/150‑1 (MQ=255)

                                                                           cGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTgcg                                                                            >  2:3101767/1‑150 (MQ=255)

                                                                            gcgcaggagcTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCACAGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTgcgc                                                                           <  2:3627924/141‑1 (MQ=255)

                                                                             cgcaGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCa                                                                          >  1:3599516/3‑150 (MQ=255)

                                                                              gcaGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGGCCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGCCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGAAAACGCTGGGCAg                                                                         >  1:1381603/2‑150 (MQ=255)

                                                                               caGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGc                                                                        <  1:981463/150‑1 (MQ=255)

                                                                               caGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGc                                                                        >  2:211150/1‑150 (MQ=25)

                                                                                 ggggCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCtt                                                                      >  1:1000293/1‑150 (MQ=255)

                                                                                  gggcTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTc                                                                     >  2:3344651/4‑150 (MQ=255)

                                                                                  gggcTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTc                                                                     <  2:1148066/147‑1 (MQ=255)

                                                                                  ggACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTc                                                                     <  2:3567147/150‑1 (MQ=255)

                                                                                   gACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGAGCGCCTGCACCGGGAAACGCTGCGCAGCTTCg                                                                    >  1:3316428/1‑150 (MQ=255)

                                                                                     cTGGCGCACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGc                                                                  <  1:2209637/150‑1 (MQ=25)

                                                                                      tGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGcc                                                                 <  1:2068116/150‑1 (MQ=255)

                                                                                          gAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTa                                                             >  1:369434/1‑150 (MQ=255)

                                                                                            aCCGCTGTATACCGGTCAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATg                                                           <  2:1036285/150‑1 (MQ=255)

                                                                                            aCCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATg                                                           >  2:2888557/1‑150 (MQ=255)

                                                                                                 tGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTc                                                      >  2:2032653/1‑150 (MQ=255)

                                                                                                 tGTAAACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTc                                                      <  2:1244246/150‑1 (MQ=255)

                                                                                                  gTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTca                                                     <  1:1523495/150‑1 (MQ=255)

                                                                                                   tataCCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTcac                                                    <  1:2164812/150‑1 (MQ=255)

                                                                                                    ataCCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTcacc                                                   >  2:2542796/1‑150 (MQ=255)

                                                                                                       ccGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCaccacc                                                <  1:802065/150‑1 (MQ=255)

                                                                                                          gACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCt                                             <  2:1898140/150‑1 (MQ=255)

                                                                                                          gACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCt                                             <  2:2977233/150‑1 (MQ=255)

                                                                                                             atccTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTtat                                          <  2:2185532/148‑1 (MQ=255)

                                                                                                             aGCCTGCCCGCCGTGGAATGGCTGTCCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTtat                                          <  2:659319/150‑1 (MQ=255)

                                                                                                             aGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGTCCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCtttt                                          >  1:1603665/1‑148 (MQ=255)

                                                                                                             aGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTtat                                          <  1:465542/150‑1 (MQ=255)

                                                                                                                cTGCCCGCCGTGGAATGGGTGACCTACGGCAGCGGTTTCCTGGCAGGCATGAAACTCGGCGGCCCACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATAcc                                       <  1:582731/150‑1 (MQ=255)

                                                                                                                cTGCCCGCCGTGGAATGGCTGACCTACGGCAGGGGTTAGCTGGCAGGCTTGAAACACGGGGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATAcc                                       <  2:2827063/150‑1 (MQ=255)

                                                                                                                cTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATAcc                                       <  1:1038174/150‑1 (MQ=255)

                                                                                                                 tGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGGTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCCCTTATAccc                                      >  1:2906523/1‑150 (MQ=255)

                                                                                                                  gCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATAcccc                                     <  2:1379515/150‑1 (MQ=255)

                                                                                                                   cccGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCt                                    <  2:588578/150‑1 (MQ=255)

                                                                                                                    ccgccgTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGGAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTg                                   >  1:3402029/1‑150 (MQ=255)

                                                                                                                    ccgccgTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACACACCACCGCTTATACCCCTg                                   <  2:1567708/150‑1 (MQ=255)

                                                                                                                      gccgTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGcc                                 >  1:2347642/1‑150 (MQ=255)

                                                                                                                       ccgTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCg                                >  2:484120/1‑150 (MQ=255)

                                                                                                                         gTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCggg                              >  2:3213945/1‑150 (MQ=255)

                                                                                                                         gTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCggg                              <  2:3864777/150‑1 (MQ=255)

                                                                                                                           ggAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCa                            <  2:3035786/150‑1 (MQ=255)

                                                                                                                           ggAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCa                            <  2:1738855/150‑1 (MQ=255)

                                                                                                                            gAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAg                           <  1:146229/150‑1 (MQ=255)

                                                                                                                               tGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACGGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTa                        <  1:1263632/150‑1 (MQ=255)

                                                                                                                               tGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTa                        >  2:1525198/1‑150 (MQ=255)

                                                                                                                                  cTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCCCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACag                     >  1:3138517/1‑150 (MQ=255)

                                                                                                                                   tGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACaga                    >  1:1219061/1‑150 (MQ=255)

                                                                                                                                      ccTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACCCCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGcc                 <  2:3980727/150‑1 (MQ=255)

                                                                                                                                       cTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCa                >  2:875322/1‑150 (MQ=255)

                                                                                                                                        tACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGGGTACACCCGCGACCGCCTGCAACGGGGAACGCTGCGCAGCTTCGGCCGGTATGTACTCACCACCGCCTATACCCCTGCCGGGCAGTTACAGTGCCAg               >  2:1808260/1‑150 (MQ=255)

                                                                                                                                        tACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGGGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGGACTCACCACCCCCTATTCCCCTGCCGGGCAGGTACAGAGCCAg               >  1:1712517/1‑150 (MQ=255)

                                                                                                                                        tACGGCAGCGCCTGGCTGTCAGGCACGGAACTCGGCGACACACCGGTGGTGGGGTACACCCGCGACCGCCTGCACCGGGAAAGGCCGCGCAGCTTCGGCCGTTATGGACTCACCACCGCTTATACCCCACCCGGGCAGTTTCAGAGCCaa               >  1:729131/1‑149 (MQ=255)

                                                                                                                                          cGGCCGCGGTTACCTGGCAGGCAAGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGGCCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCTCCGCTTATACCGCAGCCGGGCAGTTACAGAGCCAGCa             <  2:91735/150‑1 (MQ=255)

                                                                                                                                            gCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCAcc           <  2:4030372/150‑1 (MQ=255)

                                                                                                                                             cAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCTCCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCt          <  1:3023165/150‑1 (MQ=255)

                                                                                                                                             cAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCt          <  1:642119/150‑1 (MQ=255)

CAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCTGAACAGCC  >  NODE_92/162‑460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`