breseq  version 0.35.4  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsAA-5R-unknown_S3_L001_R1_001862,761198,278,48899.9%229.8 bases251 bases98.5%
total862,761198,278,48899.9%229.8 bases251 bases98.5%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistribution12,158,23892.64.7100.0%Aggregatibacter actinomycetemcomitans Aa5S
total2,158,238100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000006026
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000107
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.025

Junction Skew Score Calculation

reference sequencepr(no read start)
10.86497

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.8
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.4.1
R4.0.0

Execution Times

stepstartendelapsed
Read and reference sequence file input12:01:38 20 Jan 202212:01:58 20 Jan 202220 seconds
Read alignment to reference genome12:01:58 20 Jan 202212:02:52 20 Jan 202254 seconds
Preprocessing alignments for candidate junction identification12:02:52 20 Jan 202212:03:10 20 Jan 202218 seconds
Preliminary analysis of coverage distribution12:03:10 20 Jan 202212:03:57 20 Jan 202247 seconds
Identifying junction candidates12:03:57 20 Jan 202212:03:58 20 Jan 20221 second
Re-alignment to junction candidates12:03:58 20 Jan 202212:04:13 20 Jan 202215 seconds
Resolving best read alignments12:04:13 20 Jan 202212:04:38 20 Jan 202225 seconds
Creating BAM files12:04:38 20 Jan 202212:05:18 20 Jan 202240 seconds
Tabulating error counts12:05:18 20 Jan 202212:05:38 20 Jan 202220 seconds
Re-calibrating base error rates12:05:38 20 Jan 202212:05:39 20 Jan 20221 second
Examining read alignment evidence12:05:39 20 Jan 202212:09:44 20 Jan 20224 minutes 5 seconds
Polymorphism statistics12:09:44 20 Jan 202212:09:45 20 Jan 20221 second
Output12:09:45 20 Jan 202212:10:00 20 Jan 202215 seconds
Total 8 minutes 22 seconds