Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	1	1,308,177	Δ11 bp	intergenic (+148597/+112750)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	1	1308177	1308187	11	32 [0]	[0] 32	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1308176	0 (0.000)	32 (0.360)	23/430	2.4	100%	intergenic (+148596/+112761)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
	?	1	1308188 =	0 (0.000)					intergenic (+148608/+112749)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

CCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTCTTAACGTATTGATTTAAACAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTGGTTTTAAACTTAGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATC  >  1/1307940‑1308432                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     aagtcgtctttcccagttttctaatttggcttttttagttaccccttgaacagtttctggtagtccagctactcccaaaaggtctttcagttctagccactctcggatcatatctaacctcctagttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGtt                                                                                                                                                                                                                                                    <  1:486843‑M2/3‑1 (MQ=255)        ctttcccagttttctaatttggcttttttagttaccccttgaacagtttctggtagtccagctactcccaaaaggtctttcagttctagccactctcggatcatatctaacctcctagttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTa                                                                                                                                                                                                                                             >  1:117822‑M2/242‑251 (MQ=255)                         tttggcttttttagttaccccttgaacagtttctggtagtccagctactcccaaaaggtctttcagttctagccactctcggatcatatctaacctcctagttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAgggg                                                                                                                                                                                                                            <  1:101781‑M2/27‑1 (MQ=255)                               ttttttagttaccccttgaacagtttctggtagtccagctactcccaaaaggtctttcagttctagccactctcggatcatatctaacctcctagttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGAc                                                                                                                                                                                                                      <  1:299749‑M2/33‑1 (MQ=255)                                                                            caaaaggtctttcagttctagccactctcggatcatatctaacctcctagttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCaaa                                                                                                                                                                         <  1:364573‑M2/78‑1 (MQ=255)                                                                                                    ctctcggatcatatctaacctcctagttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGa                                                                                                                                                    >  1:258807‑M2/150‑248 (MQ=255)                                                                                                                             gttttaaaccaaggattaaaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGAt                                                                                                                        >  1:142910‑M2/125‑251 (MQ=255)                                                                                                                                               aaactaagtttaatttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGAc                                                                                                                                                                                                                      <  1:900553‑M2/33‑1 (MQ=255)                                                                                                                                                           atttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAacattac                                                                                          <  1:741275‑M2/157‑1 (MQ=255)                                                                                                                                                            tttacaatacctattaacgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGAc                                                                                                                                                                                                                      <  1:174772‑M2/33‑1 (MQ=255)                                                                                                                                                                             cgtattgatttaaataaaaataaatttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATg                                                                                                       >  1:17737‑M2/77‑220 (MQ=255)                                                                                                                                                                                                    atttctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCt                                                                                                                                                                                            <  1:335256‑M2/59‑1 (MQ=255)                                                                                                                                                                                                       tctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACAt                                              >  1:748594‑M2/51‑251 (MQ=255)                                                                                                                                                                                                       tctttacataagttttacaaaatttttatttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACAt                                              >  1:654766‑M2/51‑251 (MQ=255)                                                                                                                                                                                                                                  atttaaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGAc                                                                                                                                                                                                                      >  1:546691‑M2/24‑56 (MQ=255)                                                                                                                                                                                                                                      aaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAg                                                                         <  1:815028‑M2/174‑1 (MQ=255)                                                                                                                                                                                                                                      aaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGctc                                                       <  1:441461‑M2/192‑1 (MQ=255)                                                                                                                                                                                                                                      aaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGaa               >  1:696710‑M2/20‑251 (MQ=255)                                                                                                                                                                                                                                      aaactattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGaa               >  1:87221‑M2/20‑251 (MQ=255)                                                                                                                                                                                                                                         ctattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAAcc                                                                                                                                                                                             >  1:406427‑M2/17‑74 (MQ=255)                                                                                                                                                                                                                                         ctattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAAcc                                                                                                                                                                                             >  1:265028‑M2/17‑74 (MQ=255)                                                                                                                                                                                                                                         ctattgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAggg            >  1:107266‑M2/17‑251 (MQ=255)                                                                                                                                                                                                                                           attgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATg                                                                                                       >  1:794361‑M2/15‑158 (MQ=255)                                                                                                                                                                                                                                           attgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAacattac                                                                                          <  1:590361‑M2/157‑1 (MQ=255)                                                                                                                                                                                                                                           attgttgactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAggg            <  1:601089‑M2/235‑1 (MQ=255)                                                                                                                                                                                                                                                 gactttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAacattacat                                                                                        >  1:366964‑M2/9‑167 (MQ=255)                                                                                                                                                                                                                                                  actttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAg                                                                                                              <  1:859599‑M2/137‑1 (MQ=255)                                                                                                                                                                                                                                                   ctttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGAc                                                                                                                                                                                                                      <  1:258146‑M2/33‑1 (MQ=255)                                                                                                                                                                                                                                                   ctttagGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATc  <  1:812091‑M2/245‑1 (MQ=255)                                                                                                                                                                                                                                                        gGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAg                                                                                                              <  1:601234‑M2/137‑1 (MQ=255)                                                                                                                                                                                                                                                        gGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAg                                                                                                              >  1:316863‑M2/2‑138 (MQ=255)                                                                                                                                                                                                                                                        gGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTaaaaa                                        <  1:224090‑M2/207‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     CCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTCTTAACGTATTGATTTAAACAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTGGTTTTAAACTTAGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAGCGAAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATC  >  1/1307940‑1308432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑