Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	722,525	+GGTAAA	intergenic (‑28784/+113)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 722521	0 (0.000)	30 (0.350) +TAAAGG	21/410	2.4	100%	intergenic (‑28780/+117)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
	?	1	722522 =	0 (0.000)					intergenic (‑28781/+116)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

AATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACCAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCGATTATAGGGAAATATCGG  >  1/722323‑722757                                                                                                                                                                                                        |                                                                                                                                                                                                                                            cttgagggattatttaaagaattaaaacaaaaattaactgtgcataatggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAat                                                                                                                                                                                          <  1:764449‑M2/52‑1 (MQ=255)                              aaaattaactgtgcataatggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATac                                                                                                                                                                                                             >  1:129233‑M2/171‑203 (MQ=255)                              aaaattaactgtgcataatggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGa                                                                                                                                                             >  1:830659‑M2/171‑251 (MQ=255)                                aattaactgtgcataatggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGaaa                                                                                                                                                                                                   >  1:251235‑M2/169‑211 (MQ=255)                                aattaactgtgcataatggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGa                                                                                                                                                             >  1:571227‑M2/169‑249 (MQ=255)                                     actgtgcataatggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTAt                                                                                                                                                                                                        >  1:351670‑M2/164‑201 (MQ=255)                                     actgtgcataatggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAAtt                                                                                                                                                                                 >  1:147116‑M2/164‑224 (MQ=255)                                                tggtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAagt                                                                                                                                           >  1:591343‑M2/153‑251 (MQ=255)                                                 ggcttaacaaggaggcgtaaaatagtgtttattatcgattttctaaatagaacgagctggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAagta                                                                                                                                          >  1:875706‑M2/152‑251 (MQ=255)                                                  gtttaacaaggaggcgtaaaatagtgtttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAg                                                                                                                                                                           >  1:871317‑M2/151‑217 (MQ=255)                                                                             ttattaaggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGt                                                                                                                                                >  1:780155‑M2/124‑217 (MQ=255)                                                                                    ggattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATac                                                                                                                                                                                                             >  1:86467‑M2/117‑149 (MQ=255)                                                                                     gattttctaaatagaaagagttggtaaaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACt                                                                                                       <  1:165534‑M2/135‑1 (MQ=255)                                                                                                               aaaccaactcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGt                                                                            <  1:293933‑M2/162‑1 (MQ=255)                                                                                                                     actcttttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGtggttttggtt                                                                                                                                                                >  1:494783‑M2/84‑161 (MQ=255)                                                                                                                          tttgtctactatgcctatttttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATac                                                                                                                                                                                                             <  1:66003‑M2/33‑1 (MQ=255)                                                                                                                                             tttagtacctagaaaatcactaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATggggg                                              <  1:363407‑M2/192‑1 (MQ=255)                                                                                                                                                                ctaaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACtgctctgaccgactgagctatgggctgtctcttatacacattccg                           >  1:604938‑M2/41‑230 (MQ=255)                                                                                                                                                                  aaattcccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATg                                                                                                                           <  1:215234‑M2/115‑1 (MQ=255)                                                                                                                                                                       cccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGaaa                                                                                                                                                                                                   <  1:138883‑M2/43‑1 (MQ=255)                                                                                                                                                                        ccctgatagggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAatat                                                                                                                            >  1:542571‑M2/33‑146 (MQ=255)                                                                                                                                                                                gggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGc                                    <  1:199024‑M2/202‑1 (MQ=255)                                                                                                                                                                                gggtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAggg           >  1:699306‑M2/25‑251 (MQ=255)                                                                                                                                                                                  gtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATgg                                                                                                                                                                                                      <  1:589643‑M2/40‑1 (MQ=255)                                                                                                                                                                                  gtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGAc                                                                                                        >  1:635544‑M2/23‑156 (MQ=255)                                                                                                                                                                                  gtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAAc                                                                                                  >  1:881600‑M2/23‑162 (MQ=255)                                                                                                                                                                                  gtactatatcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGaa         >  1:486240‑M2/23‑251 (MQ=255)                                                                                                                                                                                         atcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGgaaatattgg  >  1:701487‑M2/16‑248 (MQ=255)                                                                                                                                                                                         atcaggtactaaaggTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGgaaatattgg  >  1:386206‑M2/16‑248 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                            AATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACCAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCGATTATAGGGAAATATCGG  >  1/722323‑722757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.