Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	722,525	+GGTAAA	intergenic (‑28784/+113)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 722521	0 (0.000)	30 (0.350) +TAAAGG	21/410	2.4	100%	intergenic (‑28780/+117)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
	?	1	722522 =	0 (0.000)					intergenic (‑28781/+116)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

CTTATTTACGTTTGAACAATATGCTGATTTAAACATTGAAAAAACCACTAATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/722274‑722521 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACCAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCGATTATAGGGAAATATCGG  >  1/722522‑722757                                                                                                                                                                                                                                                         ||||||                                                                                                                                                                                                                                             CTTATTTACGTTTGAACAATATGCTGATTTAAACATTGAAAAAACCACTAATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAA                                                                                                                                                                                                                                                 <  1:56156/251‑1                                                        CTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAAT                                                                                                                                                                                          <  1:764449/251‑1                                                                                     AAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGA                                                                                                                                                             >  1:830659/1‑251                                                                                     AAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATAC                                                                                                                                                                                                             >  1:129233/1‑203                                                                                       AATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGA                                                                                                                                                             >  1:571227/1‑249                                                                                       AATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAA                                                                                                                                                                                                   >  1:251235/1‑211                                                                                            ACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATT                                                                                                                                                                                 >  1:147116/1‑224                                                                                            ACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTAT                                                                                                                                                                                                        >  1:351670/1‑201                                                                                                       TGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGT                                                                                                                                           >  1:591343/1‑251                                                                                                        ggcTTAACAAGGAGGCGTAAAATAGTGTTTATTATCGATTTTCTAAATAGAACGAGCTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTA                                                                                                                                          >  1:875706/4‑251                                                                                                         GTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAG                                                                                                                                                                           >  1:871317/1‑217                                                                                                                                    TTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGT                                                                                                                                                >  1:780155/1‑217                                                                                                                                           GGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATAC                                                                                                                                                                                                             >  1:86467/1‑149                                                                                                                                            GATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACT                                                                                                       <  1:165534/250‑1                                                                                                                                                                      AAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGT                                                                            <  1:293933/251‑1                                                                                                                                                                            ACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTT                                                                                                                                                                >  1:494783/1‑161                                                                                                                                                                                 TTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATAC                                                                                                                                                                                                             <  1:66003/111‑1                                                                                                                                                                                                    TTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGG                                              <  1:363407/251‑1                                                                                                                                                                                                                       CTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGctgtctcttatacacattccg                           >  1:604938/1‑230                                                                                                                                                                                                                         AAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATG                                                                                                                           <  1:215234/153‑1                                                                                                                                                                                                                              CCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAA                                                                                                                                                                                                   <  1:138883/76‑1                                                                                                                                                                                                                               CCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATAT                                                                                                                            >  1:542571/1‑146                                                                                                                                                                                                                                       GGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGG           >  1:699306/1‑251                                                                                                                                                                                                                                       GGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGC                                    <  1:199024/226‑1                                                                                                                                                                                                                                         GTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAA         >  1:486240/1‑251                                                                                                                                                                                                                                         GTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAAC                                                                                                  >  1:881600/1‑162                                                                                                                                                                                                                                         GTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGAC                                                                                                        >  1:635544/1‑156                                                                                                                                                                                                                                         GTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGG                                                                                                                                                                                                      <  1:589643/62‑1                                                                                                                                                                                                                                                ATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATtgg  >  1:701487/1‑248                                                                                                                                                                                                                                                ATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATtgg  >  1:386206/1‑248                                                                                                                                                                                                                                                         ||||||                                                                                                                                                                                                                                             CTTATTTACGTTTGAACAATATGCTGATTTAAACATTGAAAAAACCACTAATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/722274‑722521 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACCAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCGATTATAGGGAAATATCGG  >  1/722522‑722757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.