Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	722,525	+GGTAAA	intergenic (‑28784/+113)	unknown ← / ← unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 722521	0 (0.000)	30 (0.350) +TAAAGG	21/410	2.4	100%	intergenic (‑28780/+117)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT
	?	1	722522 =	0 (0.000)					intergenic (‑28781/+116)	unknown/unknown	tRNA‑Thr‑TGT/tRNA‑Met‑CAT

CTTATTTACGTTTGAACAATATGCTGATTTAAACATTGAAAAAACCACTAATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACCAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCGATTATAGGGAAATATCGGGGTAAA  >  1/722274‑722763                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cTTATTTACGTTTGAACAATATGCTGATTTAAACATTGAAAAAACCACTAATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaa                                                                                                                                                                                                                                                 <  1:56156‑M1/251‑4 (MQ=255)                                                        cTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaat                                                                                                                                                                                          <  1:764449‑M1/251‑59 (MQ=255)                                                                                     aaaaTTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttga                                                                                                                                                             >  1:830659‑M1/1‑164 (MQ=255)                                                                                     aaaaTTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatac                                                                                                                                                                                                             >  1:129233‑M1/1‑164 (MQ=255)                                                                                       aaTTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttga                                                                                                                                                             >  1:571227‑M1/1‑162 (MQ=255)                                                                                       aaTTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaa                                                                                                                                                                                                   >  1:251235‑M1/1‑162 (MQ=255)                                                                                            aCTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatt                                                                                                                                                                                 >  1:147116‑M1/1‑157 (MQ=255)                                                                                            aCTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactat                                                                                                                                                                                                        >  1:351670‑M1/1‑157 (MQ=255)                                                                                                       tGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagt                                                                                                                                           >  1:591343‑M1/1‑146 (MQ=255)                                                                                                        ggcttaacAAGGAGGCGTAAAATAGTGTTTATTATCGATTTTCTAAATAGAACGAGCTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagta                                                                                                                                          >  1:875706‑M1/4‑145 (MQ=255)                                                                                                         gTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataag                                                                                                                                                                           >  1:871317‑M1/1‑144 (MQ=255)                                                                                                                                    ttattaAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagt                                                                                                                                                >  1:780155‑M1/1‑117 (MQ=255)                                                                                                                                           ggATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatac                                                                                                                                                                                                             >  1:86467‑M1/1‑110 (MQ=255)                                                                                                                                            gATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggact                                                                                                       <  1:165534‑M1/250‑142 (MQ=255)                                                                                                                                                                      aaaCCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagt                                                                            <  1:293933‑M1/251‑169 (MQ=255)                                                                                                                                                                            aCTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtt                                                                                                                                                                >  1:494783‑M1/1‑77 (MQ=255)                                                                                                                                                                                 tttGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTac                                                                                                                                                                                                                                                    <  1:120593/72‑1 (MQ=255)                                                                                                                                                                                 tttGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatac                                                                                                                                                                                                             <  1:66003‑M1/111‑40 (MQ=255)                                                                                                                                                                                                    tttAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagtcgactgctctgaccgactgagctatggggg                                              <  1:363407‑M1/251‑199 (MQ=255)                                                                                                                                                                                                                       cTAAATTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagtcgactgctctgaccgactgagctatgggctgtctcttatacacattccg                           >  1:604938‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                         aaaTTCCCCTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatg                                                                                                                           <  1:215234‑M1/153‑122 (MQ=255)                                                                                                                                                                                                                              ccccTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaa                                                                                                                                                                                                   <  1:138883‑M1/76‑50 (MQ=255)                                                                                                                                                                                                                               cccTGATAGGGTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatat                                                                                                                            >  1:542571‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                       gggTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagtcgactgctctgaccgactgagctatgggggcggtgtgagcgtagaattaaggcgccattataggg           >  1:699306‑M1/1‑18 (MQ=255)                                                                                                                                                                                                                                       gggTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagtcgactgctctgaccgactgagctatgggggcggtgtgagc                                    <  1:199024‑M1/226‑209 (MQ=255)                                                                                                                                                                                                                                         gTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagtcgactgctctgaccgactgagctatgggggcggtgtgagcgtagaattaaggcgccattatagggaa         >  1:486240‑M1/1‑16 (MQ=255)                                                                                                                                                                                                                                         gTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaac                                                                                                  >  1:881600‑M1/1‑16 (MQ=255)                                                                                                                                                                                                                                         gTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggac                                                                                                        >  1:635544‑M1/1‑16 (MQ=255)                                                                                                                                                                                                                                         gTACTATATCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatgg                                                                                                                                                                                                      <  1:589643‑M1/62‑47 (MQ=255)                                                                                                                                                                                                                                                atCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagtcgactgctctgaccgactgagctatgggggcggtgtgagcgtagaattaaggcgccattatagggaaatattgg  >  1:701487‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                                                atCAGGTACtaaaggtaaaactaaaatgaatcaaaaaaagcataatacactatggaaatgattaaatatgttaatttataagtggttttggtttgacttgaatcagagtgaagtagtatggattaatatgttggtgcccccaacaggacttgaacctgtgactaatcgattatgagtcgactgctctgaccgactgagctatgggggcggtgtgagcgtagaattaaggcgccattatagggaaatattgg  >  1:386206‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CTTATTTACGTTTGAACAATATGCTGATTTAAACATTGAAAAAACCACTAATCGGCTTGAGGGATTATTTAAAGAATTAAAACAAAAATTAACTGTGCATAATGGTTTAACAAGGAGGCGTAAAATAGTGTTTATTAAGGATTTTCTAAATAGAAAGAGTTGGTAAAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACCAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCGATTATAGGGAAATATCGGGGTAAA  >  1/722274‑722763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.