Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	1,982,864	Δ3 bp	intergenic (‑219/+117)	unknown ← / ← unknown	RTX toxin activating lysine‑acyltransferase (EC 2.3.1.‑)/hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1982863	0 (0.000)	21 (0.240)	19/428	2.9	100%	intergenic (‑218/+120)	unknown/unknown	RTX toxin activating lysine‑acyltransferase (EC 2.3.1.‑)/hypothetical protein
	?	1	1982867 =	0 (0.000)					intergenic (‑222/+116)	unknown/unknown	RTX toxin activating lysine‑acyltransferase (EC 2.3.1.‑)/hypothetical protein

ACAATATACAAAATAACTTACACGATCGCATTACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/1982693‑1982863 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGGTTTAGGGCGATGTCTACAATATTGTGTAATC  >  1/1982867‑1983112                                                                                                                                                                                                                                                                                                                                                                                                                                   ACAATATACAAAATAACTTACACGATCGCATGACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAAT                                                                                                                                                                        >  1:511705/1‑251    ATATACAAAATAACTTACACGATCGCATGACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAA                                                                                                                                                                     >  1:654857/1‑251                                                             CAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAG                                                                                                            >  1:598612/1‑251                                                                                                        AAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGC                                                                                                                                              <  1:753047/174‑1                                                                                                                  TATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGG                                                       >  1:264296/1‑251                                                                                                                  TATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGC                                                                                                                                      <  1:491005/172‑1                                                                                                                   ATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAG                                                         <  1:122716/248‑1                                                                                                                        ACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAG                                                                                                                                    >  1:891182/1‑168                                                                                                                             ATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTA                                            >  1:792309/1‑251                                                                                                                             ATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAA                                                                                                                                                                                >  1:521867/1‑119                                                                                                                                   CAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAG                                                           <  1:473296/230‑1                                                                                                                                   CAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGC                                                                                                                                      >  1:172852/1‑155                                                                                                                                     GGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTT                                    >  1:175716/1‑251                                                                                                                                       AATATATTCAAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGTTTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGATGGGTAATTTTAAATTTAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAG                                                           >  1:674777/1‑226                                                                                                                                       AATATATTCAAGCTTTATTATTTCGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAG                                                                                                                                    <  1:759985/153‑1                                                                                                                                                AAGCTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGATTTAGGGC                         <  1:18919/251‑1                                                                                                                                                   CTTTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAG                                                                                                                                    <  1:16271/141‑1                                                                                                                                                     TTATTATTTTGCATAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCA                                                                     <  1:180483/202‑1                                                                                                                                                                  TAATAAACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATATACTTTTTTTAGATTTAGGGCGATGTCTACAATATTGTG       >  1:828723/1‑251                                                                                                                                                                       AACCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGATTTAGGGCGATGTCTACAATATTGTGTAATC  <  1:865012/251‑1                                                                                                                                                                         CCTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGG                                                                                                          <  1:533682/145‑1                                                                                                                                                                                                                                                                                                                                                                                                                                   ACAATATACAAAATAACTTACACGATCGCATTACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/1982693‑1982863 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGGTTTAGGGCGATGTCTACAATATTGTGTAATC  >  1/1982867‑1983112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.