New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	1449278 =	58 (0.860)	18 (0.270)	7/444	4.3	21.9%	coding (1333/1494 nt)	unknown	PTS system, N‑acetylmannosamine‑specific IIC component / PTS system, N‑acetylmannosamine‑specific IIB component
	?	1	1449405 =	72 (1.090)					coding (1206/1494 nt)	unknown	PTS system, N‑acetylmannosamine‑specific IIC component / PTS system, N‑acetylmannosamine‑specific IIB component
Rejected: Coverage evenness skew score above cutoff.

ATCTTACAAGGCAACGACAATACCAACTGGCTTAAAGTCATTTGGGTGGGCATCGGCTGGGCGGCGTTGTATTATTTCAGCTTCCGTTTCCTGATTACCAAATTTAACGTCATGACCCCGGGACGTGCAGAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  1/1449530‑1449278 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tctgcACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGGTGTTGCCAATGGAAATGTTCAAAATGTCGGCGATGTAGAAGGAAACGCCGTCTAAAAAGGCGTGGAACACATACAACCAAGGAG  >  1/1449405‑1449652                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       ATCTTACAAGGCAACGACAATACCAACTGGCTTAAAGTCATTTGGGTGGGCATCGGCTGGGCGGCGTTGTATTATTTCAGCTTCCGTTTCCTGATTACCAAATTTAACGTCATGACCCCGGGACGTGCAGAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGT                                                                                                                                                                                                                                                              >  1:39923/1‑249               CGACAATACCAACTGGCTTAAAGTCATTTGGGTGGGCATCGGCTGGGCGGCGTTGTATTATTTCAGCTTCCGTTTCCTGATTACCAAATTTAACGTCATGACCCCGGGACGTGCAGAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTC                                                                                                                                                                                                                                                             >  1:8465/1‑236                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATA                                                                                                                           >  1:156648/1‑251                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATA                                                                                                                           >  1:639959/1‑251                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATA                                                                                                                           >  1:57198/1‑251                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATA                                                                                                                           >  1:449478/1‑251                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATA                                                                                                                           >  1:362436/1‑251                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATA                                                                                                                           >  1:304132/1‑251                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATA                                                                                                                           >  1:203548/1‑251                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGAC                                                                                                                                                                >  1:206983/1‑214                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGC                                                                                                                                                                                           >  1:176022/1‑187                                                                                                                                  GAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATAC                                                                                                                                                                                                <  1:60179/182‑1                                                                                                                                    AGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCGCGTGGCGGTG‑AAGGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGAC                                                                                                                                                                >  1:184940/1‑211                                                                                                                                    AGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATAC                                                                                                                          >  1:513766/1‑250                                                                                                                                                                       CGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGG                                                                                      >  1:348467/1‑251                                                                                                                                                                       CGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGG                                                                                      >  1:229193/1‑251                                                                                                                                                                       CGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACC                                                                                                 <  1:245715/240‑1                                                                                                                                                                       CGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTAT                                                                                                                                               >  1:278970/1‑194                                                                                                                                                                        GTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGGg                                                                                     >  1:618138/1‑250                                                                                                                                                                         TTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGGTG                                                                                    <  1:249123/251‑1                                                                                                                                                                                                                                                           TGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGGTGTTGCCAATGGAAATGTTCAAAATGTCGGCGATGTAGAAGGAAACGCCGTCTAAAAAGGCGTGGAACACATACAACCAAGGAG  <  1:571420/251‑1                                                                                                                                                                                                                                                           TGCACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGGTGTTGCCAATGGAAATGTTCAAAATGTCGGCGATGTAGAAGGAAACGCCGTCTAAAAAGGCGTGGAACACATACAACCAAGGAG  <  1:153575/251‑1                                                                                                                                                                                                                                                             CACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGGTGTTGCCAATGGAAATGTTCAAAATGTCGGCGATGTAGAAGGAAACGCCGTCTAAAAAGGCGTGGAACACATAC            <  1:502492/239‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       ATCTTACAAGGCAACGACAATACCAACTGGCTTAAAGTCATTTGGGTGGGCATCGGCTGGGCGGCGTTGTATTATTTCAGCTTCCGTTTCCTGATTACCAAATTTAACGTCATGACCCCGGGACGTGCAGAAGAGCAGGATGACGCGGTTGAACAACAAACGTCTTCGTTAACGGAAAATGCGCACGAGATCATTAAAGCCCTCGGCAATAGTGAGAATATTGAAAATGTGGACGCGTGCATTACCCGTCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  1/1449530‑1449278 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tctgcACGTCCCGGGGTCATGACGTTAAATTTGGTAATCAGGAAACGGAAGCTGAAATAATACAACGCCGCCCAGCCGATGCCCACCCAAATGACTTTAAGCCAGTTGGTATTGTCGTTGCCTTGTAAGATACCGAACAGCAAGAAGTCGATAAAACCGCCGGAGAAGGTGTTGCCAATGGAAATGTTCAAAATGTCGGCGATGTAGAAGGAAACGCCGTCTAAAAAGGCGTGGAACACATACAACCAAGGAG  >  1/1449405‑1449652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.