Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	1,143,909	(CACCT)4→3	intergenic (+4779/+249398)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	1143896 =	6 (0.050)	53 (0.510)	36/410	1.7	90.2%	intergenic (+4766/+249415)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
	?	1	= 1143890	6 (0.060)					intergenic (+4760/+249421)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

CTTCAAAGACGACGTTTCCGAAGTGCGTAACGGCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGCCTCACCTCACCTCACCTCACCTTGCACAACTTATATCTAAACTCAAGGAAATTTTTCAAATTAATCAACCGGAGCTGGATTTCGGTATTTATCGCATTTTAAATTCCCGCGCTGCGGAAATTGCCACCTTTCTTGAAACCACACTTCCTCAAAAAATTCAAACTGCATTGGCGAGCAATGCCGAGCAAGAAACGGCAGTTTATAACCATCTTTTCACTTTCTTTTCCCGCTATTACGA  >  1/1143640‑1144129                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                cTTCAAAGACGACGTTTCCGAAGTGCGTAACGGCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATg                                                                                                                                                                                                                                                 >  1:255540/1‑251 (MQ=255)            acgTTTCCGAAGTGCGTAACGGCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcc                                                                                                                                                                                                                                               >  1:222600‑M2/1‑240 (MQ=255)             cgTTTCCGAAGTGCGTAACGGCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacc                                                                                                                                                                                                                                     >  1:923349‑M2/1‑239 (MQ=255)                        tGCGTAACGGCATGGAATGCGGTATCGGCGTTAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGTGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCAGTCAAAATACATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAAGAAAAAAATATGCCtcgcctcacctcacctcacc                                                                                                                                                                                                                           <  1:79412/250‑1 (MQ=255)                         gCGTAACGGCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcaca                                                                                                                                                                                                                         <  1:539200‑M2/251‑25 (MQ=255)                         gCGTAACGGCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGCCTCACCTCACCTCACCTCACCtt                                                                                                                                                                                                                         >  1:411102/1‑251 (MQ=255)                                 gCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttata                                                                                                                                                                                                                  >  1:1068105‑M2/1‑219 (MQ=255)                                  cATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatc                                                                                                                                                                                                                >  1:410847‑M2/1‑218 (MQ=255)                                  cATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatc                                                                                                                                                                                                                >  1:693938‑M2/1‑218 (MQ=255)                                  cATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatc                                                                                                                                                                                                                >  1:510376‑M2/1‑218 (MQ=255)                                   aTGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatc                                                                                                                                                                                                                <  1:292608‑M2/250‑34 (MQ=255)                                   aTGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGGTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatat                                                                                                                                                                                                                 <  1:627831‑M2/249‑33 (MQ=255)                                             gTATCGGCGTGAAGAACTACAACGATGTGAAAATCGGCGACCAGATTGAAGTGTTTGAAGTGGGTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATTAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaagg                                                                                                                                                                                                     <  1:1003351‑M2/251‑45 (MQ=255)                                                          gaaCTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaat                                                                                                                                                                                        <  1:822379‑M2/251‑58 (MQ=255)                                                          gaaCTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaat                                                                                                                                                                                        <  1:330430‑M2/251‑58 (MQ=255)                                                          gaaCTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaat                                                                                                                                                                                        <  1:222656‑M2/251‑58 (MQ=255)                                                           aaCTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaatt                                                                                                                                                                                       >  1:994632‑M2/1‑193 (MQ=255)                                                             cTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactc                                                                                                                                                                                                         >  1:1086200‑M2/1‑191 (MQ=255)                                                                       gtgAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccgg                                                                                                                                                                             >  1:101511‑M2/1‑181 (MQ=255)                                                                       gtgAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGCctcacctcacctcacctcac                                                                                                                                                                                                                            <  1:489/202‑1 (MQ=255)                                                                                       aGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatc                                                                                                                                                                                                                >  1:372292‑M2/1‑165 (MQ=255)                                                                                       aGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGCCTCACCTCACCTCACCTCACCTTGCACAACTTATATCTAAACTCAAGGAAATTTTTCAAATTAATCAACCGGAGCTGGATTTCgg                                                                                                                                                           >  1:299179/1‑251 (MQ=255)                                                                                         aTTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtattt                                                                                                                                                           >  1:38193‑M2/1‑163 (MQ=255)                                                                                           tGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcg                                                                                                                                                       >  1:468189‑M2/1‑161 (MQ=255)                                                                                                      aaGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactc                                                                                                                                                                                                         <  1:134734‑M2/190‑41 (MQ=255)                                                                                                          ggTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcc                                                                                                                                                                                                                                               <  1:733572‑M2/148‑3 (MQ=255)                                                                                                                             aTCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccac                                                                                                                      <  1:1023368‑M2/250‑124 (MQ=255)                                                                                                                             aTCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccac                                                                                                                      <  1:321977‑M2/250‑124 (MQ=255)                                                                                                                             aTCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggag                                                                                                                                                                           <  1:11666‑M2/197‑71 (MQ=255)                                                                                                                             aTCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacc                                                                                                                                                                                                                                     <  1:732449‑M2/139‑13 (MQ=255)                                                                                                                                           cTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgc                                                                                                                                     >  1:55714‑M2/1‑113 (MQ=255)                                                                                                                                             aaaaGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccaca                                                                                                      <  1:50526‑M2/250‑140 (MQ=255)                                                                                                                                                              tCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGCCTCACCTCACCTCACCTCACCTTGCACAACTTATATCTAAACTCAAGGAAATTTTTCAAATTAATCAACCGGAGCTGGATTTCGGTATTTATCGCATTTTAAATTCCCGCGCTGCGGAAATTGCCACCTTTCTTGAAACCACACTTCCTCaaaaaa                                                                                    >  1:73329/1‑251 (MQ=255)                                                                                                                                                                   gtgtTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactg                                                                                <  1:989549‑M2/250‑162 (MQ=255)                                                                                                                                                                           aCCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcg                                                                        >  1:355755‑M2/1‑81 (MQ=255)                                                                                                                                                                               ctctTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaa                                                                   <  1:890430‑M2/251‑175 (MQ=255)                                                                                                                                                                               ctctTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaatt                                                                                                                                            <  1:725768‑M2/178‑102 (MQ=255)                                                                                                                                                                                 ctTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatg                                                                 <  1:663088‑M2/251‑177 (MQ=255)                                                                                                                                                                                   tttACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGCCTCACCTCACCTCACCTCACCTTGCACAACTTATATCTAAACTCAAGGAAATTTTTCAAATTAATCAACCGGAGCTGGATTTCGGTATTTATCGCATTTTAAATTCCCGCGCTGCGGAAATTGCCACCTTTCTTGAAACCACACTTCCTCAAAAAATTCAAACTGCATTgg                                                                     <  1:827088/245‑1 (MQ=255)                                                                                                                                                                                      aCATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgag                                                            <  1:685731‑M2/251‑182 (MQ=255)                                                                                                                                                                                           ggATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaaga                                                       <  1:460581‑M2/251‑187 (MQ=255)                                                                                                                                                                                           ggATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagc                                                           <  1:138926‑M2/247‑183 (MQ=255)                                                                                                                                                                                           ggATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccg                                                              <  1:730631‑M2/244‑180 (MQ=255)                                                                                                                                                                                           ggATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcg                                                                        <  1:15118‑M2/234‑170 (MQ=255)                                                                                                                                                                                           ggATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaac                                                                                                                                                                                                                       <  1:951516‑M2/91‑27 (MQ=255)                                                                                                                                                                                           ggATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctc                                                                                                                                                                                                                                   >  1:392534‑M2/1‑65 (MQ=255)                                                                                                                                                                                            gATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaa                                                      <  1:354836‑M2/251‑188 (MQ=255)                                                                                                                                                                                              tataAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaa                                                      <  1:840804‑M2/249‑188 (MQ=255)                                                                                                                                                                                                 aaaCACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggc                                                 >  1:195153‑M2/1‑59 (MQ=255)                                                                                                                                                                                                  aaCACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggca                                                >  1:149661‑M2/1‑58 (MQ=255)                                                                                                                                                                                                    cacCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggc                                                 <  1:217221‑M2/248‑193 (MQ=255)                                                                                                                                                                                                     acCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacac                                                                                                     <  1:15693‑M2/195‑141 (MQ=255)                                                                                                                                                                                                      ccATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagttt                                            <  1:444243‑M2/251‑198 (MQ=255)                                                                                                                                                                                                      ccATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagttt                                            <  1:1072889‑M2/251‑198 (MQ=255)                                                                                                                                                                                                           ttCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataac                                       >  1:132118‑M2/1‑49 (MQ=255)                                                                                                                                                                                                           ttCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataac                                       >  1:319052‑M2/1‑49 (MQ=255)                                                                                                                                                                                                            tCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataacc                                      >  1:207613‑M2/1‑48 (MQ=255)                                                                                                                                                                                                            tCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataacc                                      >  1:947328‑M2/1‑48 (MQ=255)                                                                                                                                                                                                               aTCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataaccat                                    <  1:695355‑M2/250‑206 (MQ=255)                                                                                                                                                                                                               aTCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcacc                                                                                                                                                                                                                                >  1:364761‑M2/1‑45 (MQ=255)                                                                                                                                                                                                                          cTTGACAGCTAGATTTTAAAAGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataaccatcttttcacttt                         <  1:362858‑M2/250‑217 (MQ=255)                                                                                                                                                                                                                                           aaaaGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataaccatcttttcactttcttttcccgctattacga       >  1:239527‑M2/1‑17 (MQ=255)                                                                                                                                                                                                                                           aaaaGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataaccatcttttcactttcttttcccgctattacga       >  1:110380‑M2/1‑17 (MQ=255)                                                                                                                                                                                                                                            aaaGGAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataaccatcttttcactttcttttcccgctattacgac      >  1:113128‑M2/1‑16 (MQ=255)                                                                                                                                                                                                                                                gAAAAAAATATGcctcacctcacctcaccttgcacaacttatatctaaactcaaggaaatttttcaaattaatcaaccggagctggatttcggtatttatcgcattttaaattcccgcgctgcggaaattgccacctttcttgaaaccacacttcctcaaaaaattcaaactgcattggcgagcaatgccgagcaagaaacggcagtttataaccatcttttcactttcttttcccgctattacgacaacg  <  1:652167‑M2/251‑240 (MQ=255)                                                                                                                                                                                                                                                          tGcctcacctcacctcaccttgcacaacttatatc                                                                                                                                                                                                                <  1:755257‑M2/35‑34 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                CTTCAAAGACGACGTTTCCGAAGTGCGTAACGGCATGGAATGCGGTATCGGCGTGAAGAACTACAACGATGTGAAAGTCGGCGACCAGATTGAAGTGTTTGAAGTGGTTGAGGTTAAACGTTCGATCTAATTTTTAATCTAAAAGAGCGGTCAAAATTCATAGTGTTTTGACCGCTCTTTTACATAGGATATAAACACCATATTCCATCAAAAATAACTTGACAGCTAGATTTTAAAAGGAAAAAAATATGCCTCACCTCACCTCACCTCACCTTGCACAACTTATATCTAAACTCAAGGAAATTTTTCAAATTAATCAACCGGAGCTGGATTTCGGTATTTATCGCATTTTAAATTCCCGCGCTGCGGAAATTGCCACCTTTCTTGAAACCACACTTCCTCAAAAAATTCAAACTGCATTGGCGAGCAATGCCGAGCAAGAAACGGCAGTTTATAACCATCTTTTCACTTTCTTTTCCCGCTATTACGA  >  1/1143640‑1144129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.