Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	1	1,328,979	Δ641 bp	intergenic (+169399/+91318)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	1	1328979	1329619	641	41 [0]	[0] 41	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1328978	0 (0.000)	41 (0.470)	27/426	1.9	100%	intergenic (+169398/+91959)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
	?	1	1329620 =	0 (0.000)					intergenic (+170040/+91317)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

TGTTAAAACGAATCGGCGTATCGTCTAGATTTTCCTTGTCTCGGGTCAGCATCACGTTATTTTTTATGTAGGTTTCCGACACTTTTGTGCGAATGCCTGCTTGTTTGAATTTATCTGCATTTTCCTGCAAATAAACGACAAATAAGGCTAAAAAGTCGGCTTCGGAATCAATTTTATACTGCAAAATCGCTTTGTGATGAATCAGCTCCCACAAGGCTTTTAACTCTTCATATTTGCCTTCACGCATAGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGATCGTTATCATCAATCAGCTGATTAATCAAACGCAATGCGATATTTTTC  >  1/1329372‑1329784                                                                                                                                                                                                                                                         |                                                                                                                                                                     aaactgagttttaaacacgactttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGa                                                                                                                                                                    <  1:492655‑M2/3‑1 (MQ=255) aaactgagttttaaacacgactttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGa                                                                                                                                                                    >  1:485454‑M2/249‑251 (MQ=255)   actgagttttaaacacgactttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAAt                                                                                                                                                                  <  1:865805‑M2/5‑1 (MQ=255)         ttttaaacacgactttcacctcttgtgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACAt                                                                                                                                                             <  1:858713‑M2/10‑1 (MQ=255)                  cgactttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGt                                                                                                                                                   <  1:94995‑M2/20‑1 (MQ=255)                  cgactttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGt                                                                                                                                                   <  1:605010‑M2/20‑1 (MQ=255)                  cgactttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGt                                                                                                                                                   <  1:230783‑M2/20‑1 (MQ=255)                    actttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGtt                                                                                                                                                  <  1:75313‑M2/21‑1 (MQ=255)                    actttcacctcttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGtt                                                                                                                                                  <  1:571232‑M2/21‑1 (MQ=255)                              cttttgagatttgggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGt                                                                                                                                       >  1:162789‑M2/220‑251 (MQ=255)                                          gggcaaataaacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCttt                                                                                                                           >  1:733705‑M2/208‑251 (MQ=255)                                                   aacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAAt                                                                                                                                                                  >  1:212968‑M2/199‑203 (MQ=255)                                                   aacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGAc                                                                                                                  <  1:117197‑M2/53‑1 (MQ=255)                                                   aacgttcggcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGAc                                                                                                                  <  1:176467‑M2/53‑1 (MQ=255)                                                           gcgtgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAAt                                                                                                                                                                  >  1:612904‑M2/191‑195 (MQ=255)                                                             gtgtttgattttgcgttcttcgtcttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGaa                                                                                                                                                                   <  1:446118‑M2/4‑1 (MQ=255)                                                              tgtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAgg                                                                                                       <  1:786107‑M2/64‑1 (MQ=255)                                                               gtttgattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGtttt                                                                                                                                                <  1:629056‑M2/23‑1 (MQ=255)                                                                   gattttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGc                                                                                                                       <  1:626861‑M2/48‑1 (MQ=255)                                                                      tttgcgttcttcgccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCggg                                                                                               <  1:813174‑M2/72‑1 (MQ=255)                                                                                   ccttttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaatacgtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCa                                                                                  >  1:71499‑M2/167‑251 (MQ=255)                                                                                      tttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATc                                                                                                                                          <  1:620831‑M2/29‑1 (MQ=255)                                                                                      tttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCt                                                                               <  1:364251‑M2/88‑1 (MQ=255)                                                                                       ttcgtaagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGAc                                                                                                                  <  1:185152‑M2/53‑1 (MQ=255)                                                                                           taagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGa                                                                          >  1:901634‑M2/159‑251 (MQ=255)                                                                                            aagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACAt                                                                                                                                                             >  1:410335‑M2/158‑167 (MQ=255)                                                                                            aagttatccgaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGAt                                                                         <  1:37742‑M2/94‑1 (MQ=255)                                                                                                     gaaccatccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACAt                                                                                                                                                             >  1:355201‑M2/149‑158 (MQ=255)                                                                                                         catccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCActg                                                              >  1:531151‑M2/145‑249 (MQ=255)                                                                                                          atccacgccttttgcttcaatcacaaaattcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCActg                                                              >  1:663044‑M2/144‑248 (MQ=255)                                                                                                                                     attcaaaatctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAAt                                                                                                                                                                  >  1:896995‑M2/117‑121 (MQ=255)                                                                                                                                             tctcgcctgttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGATCGTTATCATCAATCAGCTGAttatc                        <  1:7521‑M2/143‑3 (MQ=255)                                                                                                                                                     gttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGATCGTTATCATCAAtcagctgattatccgccaatt                >  1:735720‑M2/101‑241 (MQ=255)                                                                                                                                                      ttttggttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGATCGTTATCATCAatcagctgattatccgccaattt               <  1:186513‑M2/152‑12 (MQ=255)                                                                                                                                                            ttttgacgatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGATCGTTATcatcaatcagctgattatccgccaatttattgg          <  1:403156‑M2/157‑17 (MQ=255)                                                                                                                                                                   gatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgtgattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGatcgttatcatcaatcagctgattatccgccaatttattggtaatacta  >  1:371442‑M2/87‑227 (MQ=255)                                                                                                                                                                   gatataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGatcgttatcatcaatcagctgattatccgccaatttattggtaatacta  >  1:272779‑M2/87‑227 (MQ=255)                                                                                                                                                                    atataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGcc                                                                                                                              <  1:900655‑M2/41‑1 (MQ=255)                                                                                                                                                                    atataggcaaaatcaggggaataagtcccgcctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGatcgttatcatcaatcagctgattatccgccaatttattggtaatacta  >  1:490172‑M2/86‑226 (MQ=255)                                                                                                                                                                                                  cctcctgccacggggattttgatagaatttttcgggattttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGAc                                                                                                                  <  1:640665‑M2/53‑1 (MQ=255)                                                                                                                                                                                                                                         ttagtaaacacggttaGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGATCGTTATCATCAATCAGCTGAttatc                        <  1:595683‑M2/143‑3 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                     TGTTAAAACGAATCGGCGTATCGTCTAGATTTTCCTTGTCTCGGGTCAGCATCACGTTATTTTTTATGTAGGTTTCCGACACTTTTGTGCGAATGCCTGCTTGTTTGAATTTATCTGCATTTTCCTGCAAATAAACGACAAATAAGGCTAAAAAGTCGGCTTCGGAATCAATTTTATACTGCAAAATCGCTTTGTGATGAATCAGCTCCCACAAGGCTTTTAACTCTTCATATTTGCCTTCACGCATAGGAATAACATCGCCTTTGGTTTTCGCATCAGTGATTTTGCCTTTATGCAAGACTGCGGCAAAGGCATTCGGGAACAGCTGTTTCACCTGTTGATAAGCGGCACTGCTGACGAAATGATCGTTATCATCAATCAGCTGATTAATCAAACGCAATGCGATATTTTTC  >  1/1329372‑1329784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑