Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	1	1,322,748	Δ849 bp	intergenic (+163168/+97341)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	1	1322748	1323596	849	33 [0]	[0] 33	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1322747	0 (0.000)	33 (0.380)	23/426	2.3	100%	intergenic (+163167/+98190)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
	?	1	1323597 =	0 (0.000)					intergenic (+164017/+97340)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

AAATTGCCCTGATGCACCGCTACCGCCATGGCGACAAAGAAACCGATAGTGGCCAAATCGCCGAAAGGCAGCACCTGATTTCCCGGTAAGATAACGGCAACCAAAATAGTCAGCGGAATAAAAATCAAACTGGCGGACACCACTGAGGTGTGTCCAAGCAAGAGCGCCGGATCCAAGCCGATAAGAAATTCTTCGCCGCCGAAACGCGCCTGTAATTTTTTACGTGCTTGCTTGGCAATGGGTGTTAAACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCTTTTAACGGATAGACGTCGCTTCAATTAATACATCATT  >  1/1323349‑1323712                                                                                                                                                                                                                                                         |                                                                                                                    agccaatagtaccggctcctaccacaatcacatttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaAc                                                                                                                    >  1:493545‑M2/249‑250 (MQ=255)         gtaccggctcctaccacaatcacatttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGa                                                                                                           >  1:567799‑M2/241‑251 (MQ=255)                 tcctaccacaatcacatttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTa                                                                                                   >  1:80011‑M2/233‑251 (MQ=255)                     accacaatcacatttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGa                                                                                               <  1:375359‑M2/23‑1 (MQ=255)                               catttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGAt                                                                                                          >  1:68160‑M2/219‑230 (MQ=255)                               catttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCAt                                                                                     >  1:859439‑M2/219‑251 (MQ=255)                                atttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCAtt                                                                                    >  1:191295‑M2/218‑251 (MQ=255)                                atttttatcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCAtt                                                                                    >  1:813162‑M2/218‑251 (MQ=255)                                       tcgctacacccctgcgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAacaaca                                                                             >  1:805503‑M2/211‑251 (MQ=255)                                                     cgccagattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGAt                                                                                                          <  1:74236‑M2/12‑1 (MQ=255)                                                          gattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTa                                                           >  1:402464‑M2/192‑250 (MQ=255)                                                          gattaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTa                                                           >  1:652046‑M2/192‑250 (MQ=255)                                                           attaatggcgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTAc                                                          <  1:263935‑M2/60‑1 (MQ=255)                                                                   cgtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGttt                                                                                                    <  1:82354‑M2/18‑1 (MQ=255)                                                                    gtgcagacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTAcc                                                         >  1:431969‑M2/182‑242 (MQ=255)                                                                         gacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGAt                                                                                                          >  1:584577‑M2/177‑188 (MQ=255)                                                                         gacccaccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGa                                                                                               <  1:874760‑M2/23‑1 (MQ=255)                                                                              accgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATc                                                                           <  1:16234‑M2/43‑1 (MQ=255)                                                                               ccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTaac                                                                                 <  1:598786‑M2/37‑1 (MQ=255)                                                                               ccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCttt                                     >  1:655604‑M2/171‑251 (MQ=255)                                                                               ccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCttt                                     >  1:733984‑M2/171‑251 (MQ=255)                                                                               ccgtaataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCttt                                     >  1:144938‑M2/171‑251 (MQ=255)                                                                                  taataggttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaAc                                                                                                                    <  1:195043‑M2/2‑1 (MQ=255)                                                                                        gttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCTTTTAACGGAt                             <  1:880018‑M2/89‑1 (MQ=255)                                                                                        gttcgataaaagcatcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCTTTTAACGGATa                            >  1:772758‑M2/162‑251 (MQ=255)                                                                                                      tcatgcaaagggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCttttaacggatagcccgccaaaata               >  1:522105‑M2/148‑238 (MQ=255)                                                                                                                ggttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagttttttcataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCTTTTaa                                  >  1:334519‑M2/138‑221 (MQ=255)                                                                                                                  ttggaatctggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAactgcaaaatgtcttttaacggatagcccgccaaaataccgataataatcc  <  1:830556‑M2/116‑26 (MQ=255)                                                                                                                           ggtgatactttcaccaaatttttcttgttgacgcaaatatattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAActgcaaaatgtcttttaacggatagcccgccaaaataccgataataatc   <  1:308594‑M2/115‑25 (MQ=255)                                                                                                                                                                 atattccgcattggcgccgaagcgacgggaaccgatgaaattgtagtttttacataatgaataatatcctttgtgacattcgtcgcaaAc                                                                                                                    <  1:408939‑M2/2‑1 (MQ=255)                                                                                                                                                                                                               tttttacataatgaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCgg                                                                 <  1:177772‑M2/53‑1 (MQ=255)                                                                                                                                                                                                                           gaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGa                                                                                               >  1:171925‑M2/31‑53 (MQ=255)                                                                                                                                                                                                                           gaataatatcctttgtgacattcttcgcaaACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACTGCTAACTGCAAAATGTCTTTTAAc                                 >  1:283564‑M2/31‑115 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                    AAATTGCCCTGATGCACCGCTACCGCCATGGCGACAAAGAAACCGATAGTGGCCAAATCGCCGAAAGGCAGCACCTGATTTCCCGGTAAGATAACGGCAACCAAAATAGTCAGCGGAATAAAAATCAAACTGGCGGACACCACTGAGGTGTGTCCAAGCAAGAGCGCCGGATCCAAGCCGATAAGAAATTCTTCGCCGCCGAAACGCGCCTGTAATTTTTTACGTGCTTGCTTGGCAATGGGTGTTAAACCGTCCATGATTGGTTTAATGACGCGTGGCATTAACAACATCACTGCAGCGGTTTTTACCGCTAACTGCAAAATGTCTTTTAACGGATAGACGTCGCTTCAATTAATACATCATT  >  1/1323349‑1323712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑