Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	1	1,322,748	Δ849 bp	intergenic (+163168/+97341)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	1	1322748	1323596	849	33 [0]	[0] 33	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1322747	0 (0.000)	33 (0.380)	23/426	2.3	100%	intergenic (+163167/+98190)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
	?	1	1323597 =	0 (0.000)					intergenic (+164017/+97340)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

AGCCAATAGTACCGGCTCCTACCACAATCACATTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAATCAAAACAAGGCTGTAATGGTGCGCAAACTACCAAATCCCCTTTGCGGAATTCCGTCGCGCTTTTCCCTATTTCCACTACCGTACCACAAAATTCGTGCCCTAGTACGATGGGGTA  >  1/1322500‑1322863                                                                                                                                                                                                                                                        |                                                                                                                     agcCAATAGTACCGGCTCCTACCACAATCACATTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAac                                                                                                                    >  1:493545‑M1/1‑248 (MQ=255)         gTACCGGCTCCTACCACAATCACATTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatga                                                                                                           >  1:567799‑M1/1‑240 (MQ=255)                 tCCTACCACAATCACATTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggttta                                                                                                   >  1:80011‑M1/1‑232 (MQ=255)                     aCCACAATCACATTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatga                                                                                               <  1:375359‑M1/251‑24 (MQ=255)                               caTTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcat                                                                                     >  1:859439‑M1/1‑218 (MQ=255)                               caTTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgat                                                                                                          >  1:68160‑M1/1‑218 (MQ=255)                                aTTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcatt                                                                                    >  1:191295‑M1/1‑217 (MQ=255)                                aTTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcatt                                                                                    >  1:813162‑M1/1‑217 (MQ=255)                                       tCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaaca                                                                             >  1:805503‑M1/1‑210 (MQ=255)                                                     cgcCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgat                                                                                                          <  1:74236‑M1/208‑13 (MQ=255)                                                          gATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggttttta                                                           >  1:402464‑M1/1‑191 (MQ=255)                                                          gATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggttttta                                                           >  1:652046‑M1/1‑191 (MQ=255)                                                           aTTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttac                                                          <  1:263935‑M1/250‑61 (MQ=255)                                                                   cGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggttt                                                                                                    <  1:82354‑M1/200‑19 (MQ=255)                                                                    gTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttacc                                                         >  1:431969‑M1/1‑181 (MQ=255)                                                                         gACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatga                                                                                               <  1:874760‑M1/199‑24 (MQ=255)                                                                         gACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgat                                                                                                          >  1:584577‑M1/1‑176 (MQ=255)                                                                              aCCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatc                                                                           <  1:16234‑M1/214‑44 (MQ=255)                                                                               ccGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttt                                     >  1:655604‑M1/1‑170 (MQ=255)                                                                               ccGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttt                                     >  1:733984‑M1/1‑170 (MQ=255)                                                                               ccGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttt                                     >  1:144938‑M1/1‑170 (MQ=255)                                                                               ccGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaac                                                                                 <  1:598786‑M1/207‑38 (MQ=255)                                                                                  tAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAac                                                                                                                    <  1:195043‑M1/169‑3 (MQ=255)                                                                                        gTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttttaacggata                            >  1:772758‑M1/1‑161 (MQ=255)                                                                                        gTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttttaacggat                             <  1:880018‑M1/250‑90 (MQ=255)                                                                                                      tcatGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttttaacggatagcccgccaaaata               >  1:522105‑M1/1‑147 (MQ=255)                                                                                                                ggTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTTCATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttttaa                                  >  1:334519‑M1/1‑137 (MQ=255)                                                                                                                  ttGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttttaacggatagcccgccaaaataccgataataatcc  <  1:830556‑M1/251‑117 (MQ=255)                                                                                                                           ggTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttaccgctaactgcaaaatgtcttttaacggatagcccgccaaaataccgataataatc   <  1:308594‑M1/241‑116 (MQ=255)                                                                                                                                                                 atatTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCGTCGCAAac                                                                                                                    <  1:408939‑M1/90‑3 (MQ=255)                                                                                                                                                                                                               tttttACATAATGAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcgg                                                                 <  1:177772‑M1/95‑54 (MQ=255)                                                                                                                                                                                                                           gAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatgacgcgtggcattaacaacatcactgcagcggtttttactgctaactgcaaaatgtcttttaac                                 >  1:283564‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                           gAATAATATCCTTTGTGACATTCTTCGCAAaccgtccatgattggtttaatga                                                                                               >  1:171925‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                     AGCCAATAGTACCGGCTCCTACCACAATCACATTTTTATCGCTACACCCCTGCGCCAGATTAATGGCGTGCAGACCCACCGTAATAGGTTCGATAAAAGCATCATGCAAAGGGTTGGAATCTGGTGATACTTTCACCAAATTTTTCTTGTTGACGCAAATATATTCCGCATTGGCGCCGAAGCGACGGGAACCGATGAAATTGTAGTTTTTACATAATGAATAATATCCTTTGTGACATTCTTCGCAATCAAAACAAGGCTGTAATGGTGCGCAAACTACCAAATCCCCTTTGCGGAATTCCGTCGCGCTTTTCCCTATTTCCACTACCGTACCACAAAATTCGTGCCCTAGTACGATGGGGTA  >  1/1322500‑1322863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑