Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	1	1,325,601	332 bp→ACCAAATATGTTCATTAGAATG	intergenic (+166021/+95005)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	1	1325601	1325932	332	31 [0]	[0] 35	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1325600	0 (0.000)	28 (0.350) +ACCAAATATGTTCATTAGAATG	24/386	1.9	100%	intergenic (+166020/+95337)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
	?	1	1325933 =	0 (0.000)					intergenic (+166353/+95004)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

GAAAAATGTAGTCTATTTTAGAAAGTAGATTTCGTACCAAAACGCTTTTCAATTTTCCTGTAAAACCCTAACAGGACCTAGCTCCCACCCCCTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTATCCGTTCTAAACTTTCCGTAAATGGTTTTTCCTCTCTATTTCGGTGTGAAGACGATGTGATACTTACAGCTCCATTTGGTGTGTGATAGGCTTGAACCGTCATTGGATTTACTTACCATTGACTTATCCTCCTGTATCTTGAATTTTGGTTGTCAACCTTACAACAGAAGTGGAAACTATTATATTTTGGCAACAAATATTGTTTATGGTTTAGTTTCTTTAATTTTATACCCTT  >  1/1325364‑1325835                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            gaAAAATGTAGTCTATTTTAGAAAGTAGATTTCGTACCAAAACGCTTTTCAATTTTCCTGTAAAACCCTAACAGGACCTAGCTCCCACCCCCTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCT‑ATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttc                                                                                                                                                                                                                                >  1:681428‑M1/1‑236 (MQ=255)               aTTTTAGAAAGTAGATTTCGTACCAAAACGCTTTTCAATTTTCCTGTAAAACCCTAACAGGACCTAGCTCCCACCCCCTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAacc                                                                                                                                                                                                                                          <  1:25399‑M1/226‑4 (MQ=255)                                                            gTAAAACCCTAACAGGACCTAGCTCCCACCCCCTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttact                                                                                                                                                                                                      >  1:685670‑M1/1‑178 (MQ=255)                                                                                         ccccTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaag                                                                                                                                       >  1:336747‑M1/1‑149 (MQ=255)                                                                                          cccTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcacc                                                                                                                                                                                <  1:497709‑M1/209‑62 (MQ=255)                                                                                          cccTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaa                                                                                                                                                                                                                         >  1:121135‑M1/1‑148 (MQ=255)                                                                                                                      tatCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggct                                                                                                                                                              <  1:89654‑M1/199‑80 (MQ=255)                                                                                                                              cgacgaTCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataata                                                                                                  >  1:168905‑M1/1‑112 (MQ=255)                                                                                                                                   aTCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcacc                                                                                                                                                                                <  1:360054‑M1/168‑62 (MQ=255)                                                                                                                                    tCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaagg                                                                                            <  1:391486‑M1/251‑146 (MQ=255)                                                                                                                                     cTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctattt                                                                                                                                                        >  1:338890‑M1/1‑105 (MQ=255)                                                                                                                                          gTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttc                                                                                                                                                       <  1:178264‑M1/186‑87 (MQ=255)                                                                                                                                              cGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatg                                                                                                                                                                                                                   >  1:628570‑M1/1‑96 (MQ=255)                                                                                                                                                aCTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggact                                                                                                                                                                       >  1:14385‑M1/1‑94 (MQ=255)                                                                                                                                                  tttATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaaggacgctcatgagcgc                                                                              <  1:119419‑M1/251‑160 (MQ=255)                                                                                                                                                       cAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaa                                                                                                                                     >  1:229950‑M1/1‑87 (MQ=255)                                                                                                                                                           aaaaGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaaggacgctcatgagcgcccttatttt                                                                     <  1:630386‑M1/251‑169 (MQ=255)                                                                                                                                                              aGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaaggacgctcatgagcgcccttattt                                                                      <  1:628832‑M1/247‑168 (MQ=255)                                                                                                                                                              aGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataa                                                                                                 <  1:692079‑M1/220‑141 (MQ=255)                                                                                                                                                                        acacCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaac                                                                                                         <  1:311103‑M1/202‑133 (MQ=255)                                                                                                                                                                         cacCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttac                                                                                                                                                                                                       >  1:58329‑M1/1‑69 (MQ=255)                                                                                                                                                                          acCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaac                                                                                                                                          <  1:612033‑M1/167‑100 (MQ=255)                                                                                                                                                                                                  cTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaac                                                                                                                                          >  1:380337‑M1/1‑44 (MQ=255)                                                                                                                                                                                                  cTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctat                                                                                                                                                    >  1:721081‑M1/1‑44 (MQ=255)                                                                                                                                                                                                  cTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttcta                                                                                                                                                     >  1:325156‑M1/1‑44 (MQ=255)                                                                                                                                                                                                    gTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaaggacgctcatgagcgcccttattttttatctaaactttaaaattaaagtgcagattttgcttttt                             >  1:6777‑M1/1‑42 (MQ=255)                                                                                                                                                                                                       gACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaaggacgctcatgag                                                                                 <  1:89356‑M1/195‑157 (MQ=255)                                                                                                                                                                                                                          aTTAAGTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaaggacgctcatgagcgcccttattttt                                                                    >  1:23507‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                               gTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaaaggacgctcatgagcgcccttattttttatctaaactttaaaattaaagtgcagattttgctttttcaactaatgcagcgaaagccactttgt  >  1:133228‑M1/1‑15 (MQ=255)                                                                                                                                                                                                                               gTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcagaaaatcaacaataataaaa                                                                                               <  1:558624‑M1/157‑143 (MQ=255)                                                                                                                                                                                                                               gTAATAATTCTCTTAaccaaatatgttcattagaatgaatggatatctgttacttttttgctttatttatctcaccaatcggactcatttggctctatttctattattcaaaacaagaaaaaatagccaatcaatcaga                                                                                                                 >  1:480069‑M1/1‑15 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            GAAAAATGTAGTCTATTTTAGAAAGTAGATTTCGTACCAAAACGCTTTTCAATTTTCCTGTAAAACCCTAACAGGACCTAGCTCCCACCCCCTTATAGTATTCAATAAATTCAAAAATATCCAAACGACGATCTATGGTCTCGACTTTATCAATAAAAGAATACACAACACCCAATTTTTCATAAAATGCGCACTGTAGACCTAATTTATTTCGACGATTAAGTAATAATTCTCTTATCCGTTCTAAACTTTCCGTAAATGGTTTTTCCTCTCTATTTCGGTGTGAAGACGATGTGATACTTACAGCTCCATTTGGTGTGTGATAGGCTTGAACCGTCATTGGATTTACTTACCATTGACTTATCCTCCTGTATCTTGAATTTTGGTTGTCAACCTTACAACAGAAGTGGAAACTATTATATTTTGGCAACAAATATTGTTTATGGTTTAGTTTCTTTAATTTTATACCCTT  >  1/1325364‑1325835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑