Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	1,432,248	Δ4 bp	intergenic (‑11235/+11995)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1432247	0 (0.000)	23 (0.260)	21/430	2.6	100%	intergenic (‑11234/+11999)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
	?	1	1432252 =	0 (0.000)					intergenic (‑11239/+11994)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

CACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAACGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGGGGAATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGATATCTAAGGTTCTAACACCTAAAAATTTTTTATAAGCGAACAATCTTATATATCAAATTAAGCTTCTATCG  >  1/1432008‑1432482                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       aagtcaccagcttgagcgacacaacaatttggcgtatgatgagacaaggtaaattccccaaaagtgttaaagcgtccatggggcgtgtagcgtggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTc                                                                                                                                                                                                                                  <  1:534059‑M2/7‑1 (MQ=255)                 cgacacaacaatttggcgtatgatgagacaaggtaaattccccaaaagtgttaaagcgtccatggggcgtgtagcgtggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAAtt                                                                                                                                                                                                                  <  1:353600‑M2/23‑1 (MQ=255)                                    atgatgagacaaggtaaattccccaaaagtgttaaagcgtccatggggcgtgtagcgtggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACtt                                                                                                                                                                                                                                   <  1:573166‑M2/6‑1 (MQ=255)                                                                    taaagcgtccatggggcgtgtagcgtggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGc                                                                                                                                                               <  1:267202‑M2/74‑1 (MQ=255)                                                                                 gggcgtgtagcgtggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAg                                                                                                                                                   <  1:469383‑M2/86‑1 (MQ=255)                                                                                  ggcgtgtagcgtggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAggg                                                                                                                                                 >  1:189809‑M2/164‑251 (MQ=255)                                                                                           cgtggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAg                                                                                                                                         <  1:209946‑M2/96‑1 (MQ=255)                                                                                             tggagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCAt                                                                                                                                      <  1:51365‑M2/99‑1 (MQ=255)                                                                                               gagaaaatctgatattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAAt                                                                                                                                                                                                                   >  1:151176‑M2/151‑172 (MQ=255)                                                                                                          atattgatacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATg                                                                                                                                >  1:663575‑M2/140‑244 (MQ=255)                                                                                                                atacttggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCAttt                                                                                                                                                                                                                           <  1:94481‑M2/14‑1 (MQ=255)                                                                                                                     tggcttgctgaacgttcttgatggcattttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAacac                                                                                                              <  1:139352‑M2/123‑1 (MQ=255)                                                                                                                                              attttcttccaaagacggtaaaaatgacggtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGtttattta                                                                                                                                                                                                     <  1:521727‑M2/36‑1 (MQ=255)                                                                                                                                                                           gtatagaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATGCTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATAc                                                                                                                                                           <  1:490196‑M2/78‑1 (MQ=255)                                                                                                                                                                                gaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAg                                                                                                                                                   >  1:737034‑M2/70‑155 (MQ=255)                                                                                                                                                                                gaataaacaatatcaattttattcattgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGATATCTAAGGTTCTAACACCTaa                                                   >  1:282296‑M2/70‑251 (MQ=255)                                                                                                                                                                                                          tgataaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCAt                                                                                                                                      <  1:223108‑M2/99‑1 (MQ=255)                                                                                                                                                                                                             taaatcattgcattatgtcgccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACGCCAGTGCCATCAGATATCTAAGGTTCTAACACCTAAAAATTTTTTATAAGCGAACAATCTtatat                      <  1:606548‑M2/211‑1 (MQ=255)                                                                                                                                                                                                                                 ccgataaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGATATCTAAGGTTCTAACACCTAAAAATTTTTTATAAGCGAACAATCTTATATATCAAATTAAGCTTCTATCg  <  1:579898‑M2/231‑1 (MQ=255)                                                                                                                                                                                                                                      aaataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTacaac                                                                                                                          >  1:658348‑M2/16‑126 (MQ=255)                                                                                                                                                                                                                                        ataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCta                                                                                                      <  1:377494‑M2/131‑1 (MQ=255)                                                                                                                                                                                                                                        ataatcgagtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGATATCTAAGGTTCTAACACCTAAAAATTTTTTATAAGCGAACAATCTtatat                      >  1:418394‑M2/14‑224 (MQ=255)                                                                                                                                                                                                                                                gtgggATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGa                                                                         >  1:815303‑M2/6‑165 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       CACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAACGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGGGGAATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGATATCTAAGGTTCTAACACCTAAAAATTTTTTATAAGCGAACAATCTTATATATCAAATTAAGCTTCTATCG  >  1/1432008‑1432482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.