Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	1,432,248	Δ4 bp	intergenic (‑11235/+11995)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1432247	0 (0.000)	23 (0.260)	21/430	2.6	100%	intergenic (‑11234/+11999)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
	?	1	1432252 =	0 (0.000)					intergenic (‑11239/+11994)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

AAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAACGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGGGGAATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGATATCTAAGGTTCTAACACCTAAAAATTTTTTATAAGCGAACAATCTTATATATCAAATTAAGCTTCT  >  1/1432004‑1432478                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        aaGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttc                                                                                                                                                                                                                                  <  1:534059‑M1/251‑8 (MQ=255)                 cGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataatt                                                                                                                                                                                                                  <  1:353600‑M1/251‑24 (MQ=255)                                    atgatgAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatactt                                                                                                                                                                                                                                   <  1:573166‑M1/215‑7 (MQ=255)                                                                    tAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagc                                                                                                                                                               <  1:267202‑M1/251‑75 (MQ=255)                                                                                 gggCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtag                                                                                                                                                   <  1:469383‑M1/250‑87 (MQ=255)                                                                                  ggCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtaggg                                                                                                                                                 >  1:189809‑M1/1‑163 (MQ=255)                                                                                           cGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttag                                                                                                                                         <  1:209946‑M1/250‑97 (MQ=255)                                                                                             tGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcat                                                                                                                                      <  1:51365‑M1/251‑100 (MQ=255)                                                                                               gagaAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataat                                                                                                                                                                                                                   >  1:151176‑M1/1‑150 (MQ=255)                                                                                                          atatTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatg                                                                                                                                >  1:663575‑M1/1‑139 (MQ=255)                                                                                                                aTACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccattt                                                                                                                                                                                                                           <  1:94481‑M1/147‑15 (MQ=255)                                                                                                                     tGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacac                                                                                                              <  1:139352‑M1/251‑124 (MQ=255)                                                                                                                                              aTTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttattta                                                                                                                                                                                                     <  1:521727‑M1/139‑37 (MQ=255)                                                                                                                                                                           gTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatgcttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatac                                                                                                                                                           <  1:490196‑M1/152‑79 (MQ=255)                                                                                                                                                                                gAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacacacgatctatatcaggtttttactccagtgccatcagatatctaaggttctaacacctaa                                                   >  1:282296‑M1/1‑69 (MQ=255)                                                                                                                                                                                gAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtag                                                                                                                                                   >  1:737034‑M1/1‑69 (MQ=255)                                                                                                                                                                                                          tGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcat                                                                                                                                      <  1:223108‑M1/142‑100 (MQ=255)                                                                                                                                                                                                             tAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacacacgatctatatcaggtttttacgccagtgccatcagatatctaaggttctaacacctaaaaattttttataagcgaacaatcttatat                      <  1:606548‑M1/251‑212 (MQ=255)                                                                                                                                                                                                                                 ccGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacacacgatctatatcaggtttttactccagtgccatcagatatctaaggttctaacacctaaaaattttttataagcgaacaatcttatatatcaaattaagcttctatcg  <  1:579898‑M1/251‑232 (MQ=255)                                                                                                                                                                                                                                      aaataaTCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaac                                                                                                                          >  1:658348‑M1/1‑15 (MQ=255)                                                                                                                                                                                                                                        ataatCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacacacgatctatatcaggtttttactccagtgccatcagatatctaaggttctaacacctaaaaattttttataagcgaacaatcttatat                      >  1:418394‑M1/1‑13 (MQ=255)                                                                                                                                                                                                                                        ataatCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacacacgatcta                                                                                                      <  1:377494‑M1/144‑132 (MQ=255)                                                                                                                                                                                                                                                gTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacacacgatctatatcaggtttttactccagtgccatcaga                                                                         >  1:815303‑M1/1‑5 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        AAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAACGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGGGGAATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCAACACACGATCTATATCAGGTTTTTACTCCAGTGCCATCAGATATCTAAGGTTCTAACACCTAAAAATTTTTTATAAGCGAACAATCTTATATATCAAATTAAGCTTCT  >  1/1432004‑1432478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.