Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	1	1,013,721	332 bp→CATTCTAATGAACATATTTGGT		[unknown]	[unknown]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	1	1013721	1014052	332	35 [0]	[0] 31	[unknown]	[unknown]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1013720	0 (0.000)	28 (0.370) +CATTCTAATGAACATATTTGGT	24/386	1.9	100%	intergenic (‑1/‑230)	unknown/unknown	hypothetical protein/hypothetical protein
	?	1	1014053 =	0 (0.000)					coding (104/273 nt)	unknown	hypothetical protein

AAGGGTATAAAATTAAAGAAACTAAACCATAAACAATATTTGTTGCCAAAATATAATAGTTTCCACTTCTGTTGTAAGGTTGACAACCAAAATTCAAGATACAGGAGGATAAGTCAATGGTAAGTAAATCCAATGACGGTTCAAGCCTATCACACACCAAATGGAGCTGTAAGTATCACATCGTCTTCACACCGAAATAGAGAGGAAAAACCATTTACGGAAAGTTTAGAACGGATAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAGGGGGTGGGAGCTAGGTCCTGTTAGGGTTTTACAGGAAAATTGAAAAGCGTTTTGGTACGAAATCTACTTTCTAAAATAGACTACATTTTTC  >  1/1013818‑1014289                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             acaaagtggctttcgctgcattagttgaaaaagcaaaatctgcactttaattttaaagtttagataaaaaataagggcgctcatgagcgtcctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTAc                                                                                                                                                                                                                                <  1:133228‑M2/15‑1 (MQ=255)                            aaaaagcaaaatctgcactttaattttaaagtttagataaaaaataagggcgctcatgagcgtcctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTAc                                                                                                                                                                                                     <  1:6777‑M2/42‑1 (MQ=255)                                                                   aaaaataagggcgctcatgagcgtcctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAAt                                                                                                                                                                                                                           <  1:23507‑M2/20‑1 (MQ=255)                                                                    aaaataagggcgctcatgagcgtcctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCtttt                                                                                                                                                            >  1:630386‑M2/169‑251 (MQ=255)                                                                     aaataagggcgctcatgagcgtcctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCt                                                                                                                                                               >  1:628832‑M2/168‑247 (MQ=255)                                                                             gcgctcatgagcgtcctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATaaa                                                                                                                                                   >  1:119419‑M2/160‑251 (MQ=255)                                                                                ctcatgagcgtcctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTc                                                                                                                                                                                                        >  1:89356‑M2/157‑195 (MQ=255)                                                                                           cctttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGa                                                                                                                                     >  1:391486‑M2/146‑251 (MQ=255)                                                                                              ttttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTAc                                                                                                                                                                                                                                >  1:558624‑M2/143‑157 (MQ=255)                                                                                                ttattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCt                                                                                                                                                               >  1:692079‑M2/141‑220 (MQ=255)                                                                                                 tattattgttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGAtcgtcg                                                                                                                               <  1:168905‑M2/112‑1 (MQ=255)                                                                                                        gttgattttctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGgtgt                                                                                                                                                                         >  1:311103‑M2/133‑202 (MQ=255)                                                                                                                tctgattgattggctattttttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTAc                                                                                                                                                                                                                                <  1:480069‑M2/15‑1 (MQ=255)                                                                                                                                    ttcttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTg                                                                                                                                                        <  1:229950‑M2/87‑1 (MQ=255)                                                                                                                                      cttgttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAgggg                                                                                          <  1:336747‑M2/149‑1 (MQ=255)                                                                                                                                         gttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAg                                                                                                                                                                                                   <  1:380337‑M2/44‑1 (MQ=255)                                                                                                                                         gttttgaataatagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGgt                                                                                                                                                                           >  1:612033‑M2/100‑167 (MQ=255)                                                                                                                                                   atagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAg                                                                                                                                                                                                   <  1:721081‑M2/44‑1 (MQ=255)                                                                                                                                                    tagaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAg                                                                                                                                                                                                   <  1:325156‑M2/44‑1 (MQ=255)                                                                                                                                                      gaaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGAc                                                                                                                                           >  1:178264‑M2/87‑186 (MQ=255)                                                                                                                                                       aaatagagccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAg                                                                                                                                      <  1:338890‑M2/105‑1 (MQ=255)                                                                                                                                                             agccaaatgagtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGata                                                                                                                       >  1:89654‑M2/80‑199 (MQ=255)                                                                                                                                                                      agtccgattggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGt                                                                                                                                                 <  1:14385‑M2/94‑1 (MQ=255)                                                                                                                                                                               ggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGAt                                                                                                                                    >  1:360054‑M2/62‑168 (MQ=255)                                                                                                                                                                               ggtgagataaataaagcaaaaaagtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAggg                                                                                           >  1:497709‑M2/62‑209 (MQ=255)                                                                                                                                                                                                     agtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAGGGGGTGGGAGCTAGGTCCTGTTAGGGTTTTAc                                                             <  1:685670‑M2/178‑1 (MQ=255)                                                                                                                                                                                                      gtaacagatatccattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGgtg                                                                                                                                                                          <  1:58329‑M2/69‑1 (MQ=255)                                                                                                                                                                                                                  cattcattctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCg                                                                                                                                               <  1:628570‑M2/96‑1 (MQ=255)                                                                                                                                                                                                                        ttctaatgaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAggg                                                                                           <  1:121135‑M2/148‑1 (MQ=255)                                                                                                                                                                                                                               gaacatatttggtTAAGAGAATTATTACTTAATCGTCGAAATAAA‑TAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAGGGGGTGGGAGCTAGGTCCTGTTAGGGTTTTACAGGAAAATTGAAAAGCGTTTTGGTACGAAATCTACTTTCTAAAATAGACTACATTTTtc  <  1:681428‑M2/236‑1 (MQ=255)                                                                                                                                                                                                                                         ggtTAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAGGGGGTGGGAGCTAGGTCCTGTTAGGGTTTTACAGGAAAATTGAAAAGCGTTTTGGTACGAAATCTACTTTCTAAAAt                >  1:25399‑M2/4‑226 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             AAGGGTATAAAATTAAAGAAACTAAACCATAAACAATATTTGTTGCCAAAATATAATAGTTTCCACTTCTGTTGTAAGGTTGACAACCAAAATTCAAGATACAGGAGGATAAGTCAATGGTAAGTAAATCCAATGACGGTTCAAGCCTATCACACACCAAATGGAGCTGTAAGTATCACATCGTCTTCACACCGAAATAGAGAGGAAAAACCATTTACGGAAAGTTTAGAACGGATAAGAGAATTATTACTTAATCGTCGAAATAAATTAGGTCTACAGTGCGCATTTTATGAAAAATTGGGTGTTGTGTATTCTTTTATTGATAAAGTCGAGACCATAGATCGTCGTTTGGATATTTTTGAATTTATTGAATACTATAAGGGGGTGGGAGCTAGGTCCTGTTAGGGTTTTACAGGAAAATTGAAAAGCGTTTTGGTACGAAATCTACTTTCTAAAATAGACTACATTTTTC  >  1/1013818‑1014289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑