Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	1,824,929	Δ3 bp	intergenic (+43029/+10453)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1824928	0 (0.000)	21 (0.240)	19/428	2.9	100%	intergenic (+43028/+10456)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
	?	1	1824932 =	0 (0.000)					intergenic (+43032/+10452)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

ATATACAAAATAACTTACACGATCGCATTACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTATTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGGTTTAGGGCGATGTCTACAATATTGTGTAATC  >  1/1824761‑1825177                                                                                                                                                                            |                                                                                                                                                                                                                                                      acaatatacaaaataacttacacgatcgcatgacagaaaatgccaaactcattacgaaatcaaatagactatgatttaattattattgttaaacaataaatagaaatataacttatttaaccttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAAt                                                                                                                                                                        >  1:511705‑M2/172‑251 (MQ=255)    atatacaaaataacttacacgatcgcatgacagaaaatgccaaactcattacgaaatcaaatagactatgatttaattattattgttaaacaataaatagaaatataacttatttaaccttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGaa                                                                                                                                                                     >  1:654857‑M2/169‑251 (MQ=255)                                                             caaatagactatgatttaattattattgttaaacaataaatagaaatataacttatttaaccttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAg                                                                                                            >  1:598612‑M2/112‑251 (MQ=255)                                                                                                        aaatataacttatttaaccttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGc                                                                                                                                              <  1:753047‑M2/106‑1 (MQ=255)                                                                                                                  tatttaaccttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATagc                                                                                                                                      <  1:491005‑M2/114‑1 (MQ=255)                                                                                                                  tatttaaccttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAggg                                                       >  1:264296‑M2/59‑251 (MQ=255)                                                                                                                   atttaaccttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTagag                                                         <  1:122716‑M2/191‑1 (MQ=255)                                                                                                                        accttatattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATagcag                                                                                                                                    >  1:891182‑M2/53‑168 (MQ=255)                                                                                                                             atattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTaaa                                                                                                                                                                                >  1:521867‑M2/48‑119 (MQ=255)                                                                                                                             atattacaggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTa                                            >  1:792309‑M2/48‑251 (MQ=255)                                                                                                                                   caggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATagc                                                                                                                                      >  1:172852‑M2/42‑155 (MQ=255)                                                                                                                                   caggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTag                                                           <  1:473296‑M2/189‑1 (MQ=255)                                                                                                                                     ggaatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACttttttt                                    >  1:175716‑M2/40‑251 (MQ=255)                                                                                                                                       aatatattcaagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGTTTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGATGGGTAATTTTAAATTTAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTag                                                           >  1:674777‑M2/38‑226 (MQ=255)                                                                                                                                       aatatattcaagctttattatttcgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATagcag                                                                                                                                    <  1:759985‑M2/116‑1 (MQ=255)                                                                                                                                                aagctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGATTTAGGGc                         <  1:18919‑M2/223‑1 (MQ=255)                                                                                                                                                   ctttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATagcag                                                                                                                                    <  1:16271‑M2/116‑1 (MQ=255)                                                                                                                                                     ttattattttgcataataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCa                                                                     <  1:180483‑M2/179‑1 (MQ=255)                                                                                                                                                                  taataaacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATATACTTTTTTTAGATTTAGGGCGATGTCTACAATATtgtg       >  1:828723‑M2/11‑251 (MQ=255)                                                                                                                                                                       aacctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGATTTAGGGCGATGTCTACAATATTGTGTAATc  <  1:865012‑M2/246‑1 (MQ=255)                                                                                                                                                                         cctTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAggg                                                                                                          <  1:533682‑M2/142‑1 (MQ=255)                                                                                                                                                                            |                                                                                                                                                                                                                                                      ATATACAAAATAACTTACACGATCGCATTACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTATTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGGTTTAGGGCGATGTCTACAATATTGTGTAATC  >  1/1824761‑1825177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.