Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	1,824,929	Δ3 bp	intergenic (+43029/+10453)	unknown → / ← unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1824928	0 (0.000)	21 (0.240)	19/428	2.9	100%	intergenic (+43028/+10456)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT
	?	1	1824932 =	0 (0.000)					intergenic (+43032/+10452)	unknown/unknown	tRNA‑Asp‑GTC/tRNA‑Lys‑CTT

ACAATATACAAAATAACTTACACGATCGCATTACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTATTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGGTTTAGGGCGATGTCTACAATATTGTGTA  >  1/1824758‑1825174                                                                                                                                                                           |                                                                                                                                                                                                                                                       acaATATACAAAATAACTTACACGATCGCATGACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaat                                                                                                                                                                        >  1:511705‑M1/1‑171 (MQ=255)    atatACAAAATAACTTACACGATCGCATGACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaa                                                                                                                                                                     >  1:654857‑M1/1‑168 (MQ=255)                                                             cAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaag                                                                                                            >  1:598612‑M1/1‑111 (MQ=255)                                                                                                        aaaTATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagc                                                                                                                                              <  1:753047‑M1/174‑107 (MQ=255)                                                                                                                  tATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtagaggg                                                       >  1:264296‑M1/1‑58 (MQ=255)                                                                                                                  tATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagc                                                                                                                                      <  1:491005‑M1/172‑115 (MQ=255)                                                                                                                   aTTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtagag                                                         <  1:122716‑M1/248‑192 (MQ=255)                                                                                                                        aCCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcag                                                                                                                                    >  1:891182‑M1/1‑52 (MQ=255)                                                                                                                             atatTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtagagggggaagtattta                                            >  1:792309‑M1/1‑47 (MQ=255)                                                                                                                             atatTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaa                                                                                                                                                                                >  1:521867‑M1/1‑47 (MQ=255)                                                                                                                                   cAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtag                                                           <  1:473296‑M1/230‑190 (MQ=255)                                                                                                                                   cAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagc                                                                                                                                      >  1:172852‑M1/1‑41 (MQ=255)                                                                                                                                     ggAATATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtagagggggaagtatttacttttttt                                    >  1:175716‑M1/1‑39 (MQ=255)                                                                                                                                       aaTATATTCAAGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggttttgatcttcatagctatatagcagctagccccatggcaacggtagatgggtaattttaaatttagttaatcgtctgcgtaataagcaagagaggtag                                                           >  1:674777‑M1/1‑37 (MQ=255)                                                                                                                                       aaTATATTCAAGCTTTATTATTTCGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcag                                                                                                                                    <  1:759985‑M1/153‑117 (MQ=255)                                                                                                                                                aaGCTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtagagggggaagtatttactttttttagatttagggc                         <  1:18919‑M1/251‑224 (MQ=255)                                                                                                                                                   cTTTATTATTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcag                                                                                                                                    <  1:16271‑M1/141‑117 (MQ=255)                                                                                                                                                     ttattaTTTTGCATAATAAACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagca                                                                     <  1:180483‑M1/202‑180 (MQ=255)                                                                                                                                                                  taataaACCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtagagggggaagtatatactttttttagatttagggcgatgtctacaatattgtg       >  1:828723‑M1/1‑10 (MQ=255)                                                                                                                                                                       aaCCTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaagggtaattttaaattcagttaatcgtctgcgtaataagcaagagaggtagagggggaagtatttactttttttagatttagggcgatgtctacaatattgtgtaatc  <  1:865012‑M1/251‑247 (MQ=255)                                                                                                                                                                         ccTtaatttaaatgagtgattcgtagttgggtaaaaataaagtcggcttcagattatttcttatatatttttaaatttacaatgaaaaccgggatttgatcttcatagctatatagcagctagccccatggcaacggtagaaggg                                                                                                          <  1:533682‑M1/145‑143 (MQ=255)                                                                                                                                                                           |                                                                                                                                                                                                                                                       ACAATATACAAAATAACTTACACGATCGCATTACAGAAAATGCCAAACTCATTACGAAATCAAATAGACTATGATTTAATTATTATTGTTAAACAATAAATAGAAATATAACTTATTTAACCTTATATTACAGGAATATATTCAAGCTTTATTATTTTGCATAATAAACCTATTTAATTTAAATGAGTGATTCGTAGTTGGGTAAAAATAAAGTCGGCTTCAGATTATTTCTTATATATTTTTAAATTTACAATGAAAACCGGGATTTGATCTTCATAGCTATATAGCAGCTAGCCCCATGGCAACGGTAGAAGGGTAATTTTAAATTCAGTTAATCGTCTGCGTAATAAGCAAGAGAGGTAGAGGGGGAAGTATTTACTTTTTTTAGGTTTAGGGCGATGTCTACAATATTGTGTA  >  1/1824758‑1825174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.