New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	Xen14_184	= 473	0 (0.000)	44 (0.100)	30/234	1.5	100%	intergenic (–/–)	–/–	–/–
	?	Xen14_60	32 =	0 (0.000)					intergenic (–/+159)	–/hisI	–/Histidine biosynthesis bifunctional protein HisIE

AGAAAGTTGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_184/372‑473 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttttacttcaaatgaatgtttcataaactgTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGCGCATCGGGCAA  >  Xen14_60/32‑145                                                                                                                                                                                                                          AGAAAGTTGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAACGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGG                                                                    <  1:1546846/150‑1  GAAAGTTGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGC                                                                   <  1:2347534/150‑1   AAAGTTGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCG                                                                  >  1:3287737/1‑150     AGTTGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTTAGATTATGTCCAACATTTGGGGTGCAGTTCATAGCGCA                                                                >  1:2464923/1‑150        tgaaggatTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCT                                                             <  1:3214934/142‑1        TGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCT                                                             <  1:1801720/150‑1        TGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCT                                                             <  1:1801722/150‑1        TGAAAGACTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCT                                                             <  1:2883182/150‑1         GAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTT                                                            >  1:376220/1‑150          AAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCACGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTA                                                           >  1:3453628/1‑150           AAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTAT                                                          <  1:76836/150‑1           AAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTAT                                                          >  1:2276148/1‑150             GAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCC                                                        <  1:186525/150‑1               GTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCGAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGG                                                      >  1:1608096/1‑150               GTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCGAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGG                                                      >  1:1608099/1‑150                 TTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCC                                                    <  1:3283830/150‑1                    GCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTAC                                                 >  1:4228723/1‑150                     CCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAGTGTTTCATAAACTGTACCTTCAGATGTCAGGTTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACG                                                >  1:3750356/1‑150                       GTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTG                                              <  1:2203912/150‑1                        TAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGT                                             >  1:2820015/1‑150                            AATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTG                                         <  1:2233136/150‑1                             ATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGA                                        <  1:308503/150‑1                             ATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGA                                        <  1:1469704/150‑1                                  GCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATga                                   <  1:1570890/150‑3                                   CCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTATCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAAT                                  <  1:357525/150‑1                                   CCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAAT                                  <  1:357527/150‑1                                    CTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATG                                 <  1:863051/150‑1                                    CTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATG                                 <  1:2781377/150‑1                                      GCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTG                               <  1:289782/150‑1                                      GCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTG                               <  1:289779/150‑1                                        ATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTA                             <  1:3358260/150‑1                                        ATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTA                             <  1:1209710/150‑1                                         TGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAG                            >  1:3266111/1‑150                                                    GTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGC                 <  1:3911584/150‑1                                                     TAATGATTTGCAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCT                <  1:4017074/150‑1                                                     TAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCT                <  1:3469559/150‑1                                                     TAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCT                <  1:1816863/150‑1                                                        TGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGC             <  1:1661210/150‑1                                                        TGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGC             <  1:2277424/150‑1                                                         GATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGCG            <  1:3244185/150‑1                                                         GATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGCG            <  1:454362/150‑1                                                              ACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGCGCATCG       <  1:3473745/150‑1                                                                AGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGCGCATCGGG     >  1:2346125/1‑150                                                                   GGCAAGTTTTACTTCAAATGAATGTTTCATAAACTGTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGCGCATCGGGCAA  >  1:53103/1‑150                                                                                                                                                                                                                          AGAAAGTTGAAAGAGTTTTGCCGTAGAAATGATGCCTGCATGACCAGCCAGGTAATGATTTACAGCGGCAAGTTTTACTTCAAATGAATGTTTCATAAACTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_184/372‑473 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttttacttcaaatgaatgtttcataaactgTACCTTCAGATGTCAGATTATGTCCAACATTTGGGGTGCAGTTCATGGCGCATCTTATCCGGCCTACGTGTGTTGAGATAATGTGTAGGCACGATAAGCTTGCGCATCGGGCAA  >  Xen14_60/32‑145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.