BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_004302	3,547	+A	coding (307/798 nt)	r1tp05 →	ORF5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_004302	3,545	1	.	A	98.2%	1096.9 / 5.6	440	coding (305/798 nt)	r1tp05	ORF5
Reads supporting (aligned to +/- strand): ref base . (8/0); new base A (427/5); total (435/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 471 total aligned reads displayed.

AGCTGGCGAAAGTAAATTGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑G‑‑AAGCAC‑G‑‑GA‑‑GT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCATTAACCTTGC  >  NC_004302/3471‑3617

 tgatggaaaaGTAAATTGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑G‑‑a                                                                                >  1:6358570/8‑75 (MQ=255)

        ggctttaaTTGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑g                                                                         >  1:2454630/7‑75 (MQ=255)

        aaaaaggaTTGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑g                                                                         >  1:1792028/8‑75 (MQ=255)

          agatggttGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑Ga                                                                     >  1:6747937/7‑75 (MQ=255)

          aGTAAATTGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑Ga                                                                     >  1:17599885/1‑75 (MQ=255)

            gggaacgCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GA‑‑Gt                                                                 >  1:3921427/7‑75 (MQ=255)

            cagaTTGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GA‑‑Gt                                                                 >  1:11161997/4‑75 (MQ=255)

             tttaaaccAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GA‑‑Gtg                                                                >  1:12078583/7‑74 (MQ=255)

               tttGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GA‑‑Gtgta                                                              >  1:17133728/2‑72 (MQ=255)

                gggCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GA‑‑Gtgtac                                                             >  1:3694653/3‑71 (MQ=255)

                       tgagcgAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGTAC‑G‑‑GAGTGT‑‑ACATGACgg                                                      >  1:5533227/6‑75 (MQ=255)

                        cccTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGa                                                     >  1:6912111/3‑75 (MQ=255)

                         gCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGAt                                                    >  1:12989915/1‑75 (MQ=255)

                          tatttgtaTTTGA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATg                                                   >  1:15940105/8‑75 (MQ=255)

                           aagtccATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGc                                                  >  1:8414375/5‑75 (MQ=255)

                             agaggtttCAGGGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GAAAAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGc                                                  >  1:763583/6‑75 (MQ=255)

                              gttgccacA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGaa                                               >  1:6590411/8‑75 (MQ=255)

                              agctgagcA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGaa                                               >  1:8580074/8‑75 (MQ=255)

                               tttattga‑GGATGGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAg                                              >  1:5739659/8‑75 (MQ=255)

                               ttggtaga‑GGAGTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAg                                              >  1:9896263/8‑75 (MQ=255)

                               ggaagtaa‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAg                                              >  1:1227220/8‑75 (MQ=255)

                               atgaaata‑GGATTGAGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAg                                              >  1:6453336/8‑75 (MQ=255)

                                ccagtCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGc                                             >  1:5420529/5‑75 (MQ=255)

                                 taatgcgggATGGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGc                                             >  1:17266754/8‑75 (MQ=255)

                                  ttata‑GGATT‑GGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTg                                          >  1:15209703/5‑75 (MQ=255)

                                   ttggctgATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCtt                                           >  1:13449221/7‑75 (MQ=255)

                                   ttCA‑GGGTTTGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTg                                          >  1:4198856/1‑75 (MQ=255)

                                     tctaatttGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTga                                         >  1:15254907/7‑75 (MQ=255)

                                     ggctcaTTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTga                                         >  1:1183128/6‑75 (MQ=255)

                                     agatagatGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTga                                         >  1:12222861/8‑75 (MQ=255)

                                      tggatatGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaa                                        >  1:15482834/7‑75 (MQ=255)

                                      gcttttagggTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaa                                        >  1:6248066/8‑75 (MQ=255)

                                      gatgATTCGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaa                                        >  1:12621607/4‑75 (MQ=255)

                                      agtaggcgggTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaa                                        >  1:26429/8‑75 (MQ=255)

                                      aggatgggggTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaa                                        >  1:14022889/8‑75 (MQ=255)

                                       gcgtatGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaag                                       >  1:5272789/6‑75 (MQ=255)

                                       atccttGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaag                                       >  1:5622741/5‑75 (MQ=255)

                                       agcaTTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GA‑‑GTGGACATGACGGATGCGAAGCTTgaag                                       >  1:7306509/4‑75 (MQ=255)

                                       acgcctGGGTAGTATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaa                                        >  1:10573784/6‑75 (MQ=255)

                                        cctcattgTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaaga                                      >  1:4661436/8‑75 (MQ=255)

                                         cggtGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaagaa                                     >  1:8219368/4‑75 (MQ=255)

                                         cgaggatta‑taTGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaagaa                                     >  1:16888935/8‑75 (MQ=255)

                                         agtgcgcta‑taTGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaagaa                                     >  1:2234807/8‑75 (MQ=255)

                                         agcttggTA‑GATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTgaagaa                                     >  1:14662613/6‑75 (MQ=255)

                                          attaaata‑taTGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GACTGT‑‑ACATGACGGATGCGAAGCTTGaagaag                                    >  1:9694782/7‑75 (MQ=255)

                                          atcgggTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGaagaag                                    >  1:8821399/4‑75 (MQ=255)

                                           tttgtga‑tatGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGt                                   >  1:1999976/7‑75 (MQ=255)

                                           tatggTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGt                                   >  1:16066396/4‑75 (MQ=255)

                                           gcgctaa‑tatGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGt                                   >  1:1478164/7‑75 (MQ=255)

                                           ctGGGAA‑TAGGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGt                                   >  1:5855999/2‑75 (MQ=255)

                                            tcttta‑tataAAGAAGTCC‑‑TCCCAACAATCCGG‑‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTa                                  >  1:7396215/5‑75 (MQ=255)

                                            cagtga‑tatGTAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTa                                  >  1:3499712/6‑75 (MQ=255)

                                            aagaca‑tatTAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTa                                  >  1:11390081/6‑75 (MQ=255)

                                             tttta‑taTGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTAc                                 >  1:10991934/4‑75 (MQ=255)

                                             gacagtaatGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTa                                  >  1:14531195/8‑75 (MQ=255)

                                              gtgtgattGAAG‑AGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACt                                >  1:3198984/8‑75 (MQ=255)

                                              ggggagatGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTAc                                 >  1:456781/7‑75 (MQ=255)

                                              ggTA‑AATGATGAAGTCC‑‑TCCCAACAATCA‑GA‑AAGAAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACt                                >  1:11300626/1‑75 (MQ=255)

                                              agTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACt                                >  1:13577482/2‑75 (MQ=255)

                                               tgatttcgaaggaGTCC‑‑TCCCAACAATCC‑GA‑AAGCACGG‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTAGAAGAAGTAc                                 >  1:5975046/8‑75 (MQ=255)

                                               gacgcatgatgAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACt                                >  1:1072751/6‑75 (MQ=255)

                                                gtctgtGAAG‑AGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGc                              >  1:16209719/6‑75 (MQ=255)

                                                cccctgaaagaagTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTg                               >  1:15629114/8‑75 (MQ=255)

                                                   tttGAAGAAGT‑‑‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                          >  1:10784660/3‑75 (MQ=255)

                                                   tggtgggaagTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCtt                            >  1:12386184/7‑75 (MQ=255)

                                                   gtgggagaatTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCtt                            >  1:16148470/6‑75 (MQ=255)

                                                   actgagcaagTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCtt                            >  1:4340727/8‑75 (MQ=255)

                                                   aatGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCtt                            >  1:7934452/2‑75 (MQ=255)

                                                   aaataagaagTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCtt                            >  1:3992084/5‑75 (MQ=255)

                                                    gacggggagTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTa                           >  1:7025490/8‑75 (MQ=255)

                                                     tGAAGAAGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                          >  1:7164004/1‑75 (MQ=255)

                                                     agaagaaGATC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                          >  1:9351820/2‑75 (MQ=255)

                                                      ttgtaagTGC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAAt                         >  1:11700317/5‑75 (MQ=255)

                                                      gaaggaGTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAA‑‑CCTGCTTAATcc                       <  1:3346714/75‑1 (MQ=255)

                                                       gatatgTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATc                        >  1:13136525/6‑75 (MQ=255)

                                                       cagaagTCCTTTCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                          >  1:10057518/2‑75 (MQ=255)

                                                        agaagTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATcc                       >  1:889608/1‑75 (MQ=255)

                                                         gatgTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCa                      >  1:15147543/4‑75 (MQ=255)

                                                         aaggccc‑‑tccCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCa                      >  1:14960110/6‑75 (MQ=255)

                                                          aaatcc‑‑tccCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAg                     >  1:7803073/4‑75 (MQ=255)

                                                           ggatgcttccCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCa                      >  1:2984787/8‑75 (MQ=255)

                                                           ggaattgtccCCACAATCC‑GA‑AAGCAC‑GTAGT‑‑GT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTAAATCCa                      >  1:9578323/8‑75 (MQ=255)

                                                           ggTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGa                    >  1:16309898/2‑75 (MQ=255)

                                                            gTCC‑‑TCCCAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGC‑TAATccctcag                  <  1:12824992/75‑6 (MQ=255)

                                                               agaattgaacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATa                  >  1:10227429/8‑75 (MQ=255)

                                                               aaggatcaacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATa                  >  1:15771432/7‑75 (MQ=255)

                                                                actagcaacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATac                 >  1:12666042/6‑75 (MQ=255)

                                                                  ttctagaaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATacac               >  1:1675736/8‑75 (MQ=255)

                                                                  ttagaacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATacac               >  1:13640443/5‑75 (MQ=255)

                                                                  tgtatgaaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATacac               >  1:1383380/8‑75 (MQ=255)

                                                                  tagcaacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATacac               >  1:16617879/4‑75 (MQ=255)

                                                                  aagatacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATacac               >  1:9197780/6‑75 (MQ=255)

                                                                   gtaaacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACt              >  1:5021242/4‑75 (MQ=255)

                                                                   aagttagaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACt              >  1:17375395/8‑75 (MQ=255)

                                                                    ccAACAATCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTc             >  1:17493968/1‑75 (MQ=255)

                                                                     caacaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCa            >  1:2783294/1‑75 (MQ=255)

                                                                      taaaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCAt           >  1:11547056/4‑75 (MQ=255)

                                                                       tgaaTCC‑GA‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCAtt          >  1:1539878/3‑75 (MQ=255)

                                                                        catagc‑G‑‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCATTaa        >  1:8057232/6‑75 (MQ=255)

                                                                         tatattg‑‑AAGCAC‑G‑‑GAGTGT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCATTaa        >  1:12021278/7‑75 (MQ=255)

AGCTGGCGAAAGTAAATTGCCAAGTGCTGAGCCATTTCA‑GGATTGGGTA‑TATGAAGAAGTCC‑‑TCCCAACAATCC‑G‑‑AAGCAC‑G‑‑GA‑‑GT‑‑ACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCATTAACCTTGC  >  NC_004302/3471‑3617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`