| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Intron | 927 | 0 | C | T | 44.8% | 13.4 / 13.7 | 35 | intergenic (–/–) | –/– | –/– |
| Reads supporting (aligned to +/- strand): ref base C (16/0); new base T (13/0); total (35/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTAGTCTATAT‑GGAAACATATAGCAGTTCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGACGTAGA > Intron/854‑997 | gtaattttataT‑GGAAACATATAGCAGTTCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAC‑g > 1:11505635/8‑75 (MQ=255)gaccctgtataTGGGAAACATATAGCAGTTCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAc > 1:1761800/8‑75 (MQ=25)agaactttataT‑GGAAACATATAGCAGTTCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAC‑g > 1:15064372/8‑75 (MQ=255) ctagtctaTAT‑GGAAACATATAGCAGTTCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAC‑gt > 1:14641498/1‑74 (MQ=255) tgaaatataT‑CGAAACATATAGCAGATCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAC‑gta > 1:10263855/6‑73 (MQ=25) agtctaTAT‑GGAAACATATAGCAGTTCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAC‑gtat > 1:7109250/1‑72 (MQ=255) tgtatactaAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAg > 1:8697546/8‑75 (MQ=11) gtaatcctaAAC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAg > 1:13067853/8‑75 (MQ=255) ggatctataAACTGAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTag > 1:17257988/6‑74 (MQ=11) gcagttataGAC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAg > 1:1378678/6‑75 (MQ=11) aggaaagtaAAA‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAg > 1:843344/8‑75 (MQ=11) acgtgaataAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAg > 1:15195587/7‑75 (MQ=11) aacaaTATAGAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAg > 1:17407155/4‑75 (MQ=11) aaactcttaAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCAAAACGAATTAAg > 1:11124384/8‑75 (MQ=11) gtattttaAAG‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGa > 1:494971/7‑75 (MQ=11) gctccttaAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGa > 1:16560988/7‑75 (MQ=11) caaaTATAACT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGa > 1:15778844/3‑75 (MQ=11) aatgcataAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGa > 1:662059/6‑75 (MQ=11) tgttttaAAA‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGATTTAAGAc > 1:16414780/6‑75 (MQ=11) tgtaagtaaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:7575077/8‑75 (MQ=255) tgcaaggaaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:3188531/8‑75 (MQ=255) tgagggaaaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:3110383/7‑75 (MQ=255) tgacacgaaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:12038236/8‑75 (MQ=255) tgaacgcaaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:9230846/8‑75 (MQ=255) tctgttaAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:3280117/6‑75 (MQ=11) taagaaaaaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:16398788/7‑75 (MQ=255) gctccttaaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:16586762/8‑75 (MQ=255) gcgaataAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACTAATTAAGaa > 1:16821643/5‑74 (MQ=11) gcgaataAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:16217825/5‑75 (MQ=11) gatggtaAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:11110256/6‑75 (MQ=11) gAATAGAAAA‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGAc > 1:15564786/1‑75 (MQ=11) cgaattaAAG‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACAAATTAAGAc > 1:15794227/6‑75 (MQ=11) attactaAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGACGAACTCTAAACGAATTAAGAc > 1:17385311/6‑75 (MQ=11) gagttaAAG‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGACg > 1:11362473/5‑75 (MQ=11) atgttaAAT‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACCCTAAACGAATTAAGACg > 1:8877983/5‑75 (MQ=11) agggggaC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAAC‑GTGGAACTCTAAACGAATTAAGACGTa > 1:7077251/7‑75 (MQ=11) agatc‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGACGTAGa > 1:3901399/5‑75 (MQ=12) agatc‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGACGTAGa > 1:1287211/5‑75 (MQ=12) | TCTAGTCTATAT‑GGAAACATATAGCAGTTCATAAGAGAACGGTTATAGATTAACGACCTATGACGAATATAAAC‑GAAACCTAAAAAACTAGCTCAGTATAAACCTCGTGAAAACGGTGGAACTCTAAACGAATTAAGACGTAGA > Intron/854‑997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |