BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_004302	3,547	+A	coding (307/798 nt)	r1tp05 →	ORF5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_004302	3,545	1	.	A	99.1%	1095.2 / ‑0.0	436	coding (305/798 nt)	r1tp05	ORF5
Reads supporting (aligned to +/- strand): ref base . (4/0); new base A (426/6); total (430/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 464 total aligned reads displayed.

AGCTGGCGAAAGTAAATTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCG‑A‑AGCACGGA‑‑GTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCATTAAC  >  NC_004302/3471‑3612

tgatGGCGACAGTAAATTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCg                                                                         >  1:12878040/4‑75 (MQ=255)

 tgccaggAAAGTAAATTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCG‑a                                                                       >  1:3372300/7‑75 (MQ=255)

  ggagCGAAAG‑AAATTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCG‑a‑aa                                                                    >  1:15723173/4‑74 (MQ=255)

  gatgtagaaGTAAATTGCCAAGTGCTGAGCCATTTCAGGATTGCGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCG‑a‑a                                                                     >  1:13102804/8‑75 (MQ=255)

    gtagAAAGTAAATTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCG‑acag                                                                    >  1:1523065/4‑72 (MQ=255)

    ggCGAAAGTAAATTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCG‑a‑aag                                                                   >  1:10455383/1‑73 (MQ=255)

         ggcgttgttGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACgg                                                               >  1:407750/8‑75 (MQ=255)

         ataagtaTTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACgg                                                               >  1:15449298/7‑75 (MQ=255)

          ccaatgttGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGa                                                              >  1:11715148/7‑75 (MQ=255)

              taaagCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGA‑‑Gtgt                                                        >  1:9714947/5‑73 (MQ=255)

                 gcccAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGA‑‑Gtgtaca                                                     >  1:12197836/3‑70 (MQ=255)

                      attGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACg                                                  >  1:6364605/3‑75 (MQ=255)

                       gtcgataGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACgg                                                 >  1:7029627/7‑75 (MQ=255)

                        gtgagtaccATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGa                                                >  1:16299272/8‑75 (MQ=255)

                        gcgaatgCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGa                                                >  1:11897747/7‑75 (MQ=255)

                          ttcaagcATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATg                                              >  1:6265936/7‑75 (MQ=255)

                          ggcagagaTTTCAGGATTGGGTA‑TTTGAAGAAAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGAt                                               >  1:15546797/8‑75 (MQ=255)

                           tGAGCCATGTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGc                                             >  1:8342978/1‑75 (MQ=255)

                           ggcaacATTTGAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGc                                             >  1:16408021/6‑75 (MQ=255)

                           gcagtaaTTTCGGGATTGGGTA‑TATGAAG‑AAGTCATCAC‑AAC‑AAACCG‑ATAGCACTGAGTGTACATGACGGATGc                                             >  1:9152331/7‑75 (MQ=255)

                             tcagaTTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGa                                           >  1:7655539/5‑75 (MQ=255)

                               tgcatataGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAg                                         >  1:9038732/8‑75 (MQ=255)

                               taggggcAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAg                                         >  1:292254/7‑75 (MQ=255)

                                agaatgaGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGc                                        >  1:16669044/7‑75 (MQ=255)

                                agaatgaGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGc                                        >  1:10288540/7‑75 (MQ=255)

                                 gacagaGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCt                                       >  1:3614354/6‑75 (MQ=255)

                                 aagtgaGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCt                                       >  1:13942986/6‑75 (MQ=255)

                                   tgtaGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTg                                     >  1:10690823/4‑75 (MQ=255)

                                   attctgATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGACGCGAAGCTTg                                     >  1:8780474/6‑75 (MQ=255)

                                   agtggtgttGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTg                                     >  1:8895083/8‑75 (MQ=255)

                                   acagagATTGAGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTg                                     >  1:9342110/6‑75 (MQ=255)

                                   aatatgATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTg                                     >  1:10980655/6‑75 (MQ=255)

                                    tcttcaTTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGC‑AAGCTTgaa                                   >  1:4715835/6‑75 (MQ=255)

                                     ttctgaagggTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:13819043/8‑75 (MQ=255)

                                     tatgaatGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:311315/7‑75 (MQ=255)

                                     taaaaTTGTGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:15650597/5‑75 (MQ=255)

                                     gtgtactGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:3770260/7‑75 (MQ=255)

                                     ggagtgtGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:5084926/7‑75 (MQ=255)

                                     ggagtgtGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:10201869/7‑75 (MQ=255)

                                     gccgggggggTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:7840069/8‑75 (MQ=255)

                                     atatggggggTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:14641665/8‑75 (MQ=255)

                                     acacagtGGGTA‑‑ATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaag                                  >  1:9491318/7‑75 (MQ=255)

                                     aatgATTGGTTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaa                                   >  1:12050893/4‑75 (MQ=255)

                                      atgATTGGGGA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaag                                  >  1:7979297/3‑75 (MQ=255)

                                      actatcgggTA‑GATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaag                                  >  1:3182527/7‑75 (MQ=255)

                                      acgaatGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaag                                  >  1:14028238/6‑75 (MQ=255)

                                       agATTGGGCG‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaaga                                 >  1:10112149/2‑75 (MQ=255)

                                        gtaagggTATTATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaaga                                 >  1:298305/5‑75 (MQ=255)

                                        gactGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaagaa                                >  1:12768506/4‑75 (MQ=255)

                                        aagtaaata‑taTGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTgaagaa                                >  1:5266273/8‑75 (MQ=255)

                                         tgatgcta‑taTGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGaagaag                               >  1:14270616/7‑75 (MQ=255)

                                         gtgctcta‑taTGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGaagaag                               >  1:17043191/7‑75 (MQ=255)

                                          atttata‑taGGAAG‑AAGTCCTCCC‑AACAAATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGaagaag                               >  1:13577058/6‑75 (MQ=255)

                                           tgcgatgtatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGt                              >  1:4309571/8‑75 (MQ=255)

                                           tacgtggtatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGt                              >  1:592613/8‑75 (MQ=255)

                                           atggTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTa                             >  1:2496402/3‑75 (MQ=255)

                                           aggggggtatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGt                              >  1:10035044/8‑75 (MQ=255)

                                           aggaca‑tatCAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTa                             >  1:13008411/6‑75 (MQ=255)

                                           aaatatttatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGt                              >  1:8258637/8‑75 (MQ=255)

                                            gcgccaaatgatg‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTa                             >  1:13505139/8‑75 (MQ=255)

                                             tgTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGaagaagaaat                           >  1:2054136/2‑71 (MQ=255)

                                             ggaggaatGAGG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTAc                            >  1:13226452/7‑75 (MQ=255)

                                             atctgtatGACG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTAc                            >  1:68951/6‑75 (MQ=255)

                                             atctgtatGACG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTAc                            >  1:1793185/6‑75 (MQ=255)

                                             atatggatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTAc                            >  1:4883762/7‑75 (MQ=255)

                                              ggtaaaagaag‑aaGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAATGTACATGACGGATGCGAAGCTTGAAGAAGTACt                           >  1:2016305/8‑75 (MQ=255)

                                              aagccatgatg‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACt                           >  1:8694303/6‑75 (MQ=255)

                                               taataagaag‑gaGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTg                          >  1:15065189/7‑75 (MQ=255)

                                               ta‑taTGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGaaaaagaagtac                         <  1:14328082/75‑7 (MQ=255)

                                               gaatacgaag‑aaGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTg                          >  1:8075540/7‑75 (MQ=255)

                                               gaagatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTg                          >  1:12675625/5‑75 (MQ=255)

                                               gaagatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTg                          >  1:13769869/5‑75 (MQ=255)

                                               acatgttaag‑aagTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTg                          >  1:2561777/8‑75 (MQ=255)

                                               acaattGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTg                          >  1:14715055/6‑75 (MQ=255)

                                                gagatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGc                         >  1:71410/4‑75 (MQ=255)

                                                agcacgaag‑aaGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGc                         >  1:9947494/6‑75 (MQ=255)

                                                a‑tatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCt                        >  1:11526916/1‑75 (MQ=255)

                                                a‑tatGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCt                        >  1:13378791/1‑75 (MQ=255)

                                                 atctcgtg‑aagTCCCCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCt                        >  1:5722280/8‑75 (MQ=255)

                                                  ccggagg‑aagTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCtt                       >  1:16173737/7‑75 (MQ=255)

                                                   taagag‑aagTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACT‑CTTaa                     >  1:8584631/5‑75 (MQ=255)

                                                   atGAAG‑AAGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTa                      >  1:1416501/1‑75 (MQ=255)

                                                   aggaag‑aaGTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTa                      >  1:6106930/3‑75 (MQ=255)

                                                    aaacg‑aagTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                     >  1:6080252/5‑75 (MQ=255)

                                                    aaacg‑aagTCCTCCC‑AAC‑AATACGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                     >  1:11181134/5‑75 (MQ=255)

                                                     ggag‑aagTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAAt                    >  1:12399557/3‑75 (MQ=255)

                                                     aaacgcggTCCTCCC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                     >  1:10893409/8‑75 (MQ=255)

                                                      tgaattgTCCTCCCAAAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTaa                     >  1:2774464/7‑75 (MQ=255)

                                                       ggggtaacctccCAAAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAAt                    >  1:14858889/8‑75 (MQ=255)

                                                         gcgacaatccC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCa                 >  1:7743868/8‑75 (MQ=255)

                                                            gatttatc‑aac‑aaTCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGAt              >  1:5409691/8‑75 (MQ=255)

                                                              gggacc‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATac            >  1:11580466/5‑75 (MQ=255)

                                                              agcatc‑aac‑aaTCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATac            >  1:720521/6‑75 (MQ=255)

                                                               ctccC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAAATaaa           <  1:15851547/75‑3 (MQ=255)

                                                                tccC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGTTTAATCCaaatctgt          <  1:7353221/75‑8 (MQ=255)

                                                                tccC‑AAC‑AATCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATacac          >  1:16838062/1‑75 (MQ=255)

                                                                gagttagc‑aaTCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATaca           >  1:1453856/8‑75 (MQ=255)

                                                                 tgc‑aac‑aaTCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACt         >  1:4465322/3‑75 (MQ=255)

                                                                 gtc‑aac‑aaTCCGAA‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACt         >  1:654432/3‑75 (MQ=255)

                                                                         aggggg‑A‑AGCACGGAGTGTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCATTAAc  >  1:6769481/6‑75 (MQ=255)

AGCTGGCGAAAGTAAATTGCCAAGTGCTGAGCCATTTCAGGATTGGGTA‑TATGAAG‑AAGTCCTCCC‑AAC‑AATCCG‑A‑AGCACGGA‑‑GTACATGACGGATGCGAAGCTTGAAGAAGTACTGCTTAATCCAGATACACTCATTAAC  >  NC_004302/3471‑3612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`