Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NODE_4	17,642	C→A	intergenic (+1428/+302)	chaA → / ← kdsA	sodium‑potassium/proton antiporter ChaA/3‑deoxy‑8‑phosphooctulonate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_4	17,642	0	C	A	100.0%	33.5 / NA	23	intergenic (+1428/+302)	chaA/kdsA	sodium‑potassium/proton antiporter ChaA/3‑deoxy‑8‑phosphooctulonate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/14);  total (9/14)

CCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTAAAACCTGACAACAGAACGGGGGCTAATCTTGAAACCAG‑ACACTTCAAGAATAGCCGCAAAAT  >  NODE_4/17498‑17662                                                                                                                                                  |                     ccACACCAAGCCGGCGTTATCCGGTGAGGTGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCaa                 <  1:3205228/150‑1 (MQ=11)  cccacCAAGCCGGCGTTATGGGGTGAGGCGCAATGTTGCGGGGGCATTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAg                <  1:444518/148‑1 (MQ=11)  cacaCCAAGCCGGCGTTATCCGGTCAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGATGAGAGGTACAACCTGACGACAGAACGGGGGCTAATCTTGAACCAAA‑ACAAATCAAg                >  1:2383337/1‑150 (MQ=11)    cacCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGaa              <  1:3473510/150‑1 (MQ=11)    cacCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGaa              <  1:955401/150‑1 (MQ=11)     acCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAAt             <  2:3361245/150‑1 (MQ=11)     acCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAAt             >  1:600592/1‑150 (MQ=11)      ccAAGCCGGCGTTATCCGGTGGGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATa            <  2:116946/150‑1 (MQ=11)      ccAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATa            <  2:1282743/150‑1 (MQ=11)       cAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAg           <  1:1504070/150‑1 (MQ=11)       cAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGGTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAAATCAAGAATAg           >  1:2925825/1‑150 (MQ=11)        aaGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGc          <  1:1860323/150‑1 (MQ=11)        aaGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGc          <  2:2753101/150‑1 (MQ=11)         ccgacttgTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGcc         <  1:2232093/143‑1 (MQ=11)          gCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCg        <  2:580379/150‑1 (MQ=11)            cGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCa      <  1:486615/150‑1 (MQ=11)            cGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCa      >  1:2399348/1‑150 (MQ=11)            cGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCCCa      >  1:824617/1‑150 (MQ=11)                gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGccaagtcgg  >  2:2232093/1‑143 (MQ=11)                gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCAAAAt  >  2:87288/1‑150 (MQ=11)                gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCAAAAt  >  2:1042486/1‑150 (MQ=11)                gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCAAAAt  <  1:2787782/150‑1 (MQ=11)                gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCCCACGTTGGGGGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑GTCCAGAACAATTCAAGAATAGCCGCAAAAt  >  1:599727/1‑150 (MQ=11)                                                                                                                                                  |                     CCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTAAAACCTGACAACAGAACGGGGGCTAATCTTGAAACCAG‑ACACTTCAAGAATAGCCGCAAAAT  >  NODE_4/17498‑17662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑