| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NODE_4 | 17,634 | Δ1 bp | intergenic (+1420/+310) | chaA → / ← kdsA | sodium‑potassium/proton antiporter ChaA/3‑deoxy‑8‑phosphooctulonate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NODE_4 | 17,632 | 0 | A | . | 96.7% | 50.0 / ‑1.5 | 30 | intergenic (+1418/+312) | chaA/kdsA | sodium‑potassium/proton antiporter ChaA/3‑deoxy‑8‑phosphooctulonate synthase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (12/17); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TGAGTCGTAGTATCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTAAAACCTGACAACAGAACGGGGGCTAATCTTGAAACCAG‑ACACTTCAAGAATAGCCGCAAAAT > NODE_4/17485‑17662 | tGAGTCGTAGTATCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTGaac > 2:469521/1‑149 (MQ=37)tGAGTCGTAGTATCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTGaac < 1:2946724/150‑2 (MQ=37) gAGTCGTAGTATCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTGaacc > 1:2002375/1‑148 (MQ=37) gtagtaTCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAG‑aac > 2:3961047/1‑148 (MQ=255) gtagtaTCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAG‑aac < 2:2267059/150‑3 (MQ=255) gtaTCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGGTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGGACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAG‑aacaat > 1:1170490/1‑145 (MQ=11) gtaTCCACACCAACTCGGCGTTATGCGGTGAGGCGCAATGTTGCGGGGGCTTTATCACTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAG‑aacaat < 1:58847/150‑6 (MQ=11) aTCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTGatccagaacaattc > 2:4054921/1‑137 (MQ=11) aTCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAG‑aacaattc > 1:4051866/1‑143 (MQ=11) aTCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAG‑aacaattc < 1:3650706/150‑8 (MQ=11) aTCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGGTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAG‑aacaattc > 2:2166753/1‑143 (MQ=11) ccACACCAAGCCGGCGTTATCCGGTGAGGTGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCaa < 1:3205228/150‑1 (MQ=11) cccacCAAGCCGGCGTTATGGGGTGAGGCGCAATGTTGCGGGGGCATTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAg < 1:444518/148‑1 (MQ=11) cacaCCAAGCCGGCGTTATCCGGTCAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGATGAGAGGTACAACCTGACGACAGAACGGGGGCTAATCTTGAACCAAA‑ACAAATCAAg > 1:2383337/1‑150 (MQ=11) cacCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGaa < 1:3473510/150‑1 (MQ=11) cacCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGaa < 1:955401/150‑1 (MQ=11) acCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAAt > 1:600592/1‑150 (MQ=11) acCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAAt < 2:3361245/150‑1 (MQ=11) ccAAGCCGGCGTTATCCGGTGGGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATa < 2:116946/150‑1 (MQ=11) ccAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATa < 2:1282743/150‑1 (MQ=11) cAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAg < 1:1504070/150‑1 (MQ=11) cAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGGTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAAATCAAGAATAg > 1:2925825/1‑150 (MQ=11) aaGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGc < 2:2753101/150‑1 (MQ=11) aaGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGc < 1:1860323/150‑1 (MQ=11) ccgacttgTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGcc < 1:2232093/143‑1 (MQ=11) gCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCg < 2:580379/150‑1 (MQ=11) cGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCa < 1:486615/150‑1 (MQ=11) cGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCa > 1:2399348/1‑150 (MQ=11) cGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCCCa > 1:824617/1‑150 (MQ=11) gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGccaagtcgg > 2:2232093/1‑143 (MQ=11) gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCAAAAt > 2:87288/1‑150 (MQ=11) gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCAAAAt > 2:1042486/1‑150 (MQ=11) gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑AACCAGAACAATTCAAGAATAGCCGCAAAAt < 1:2787782/150‑1 (MQ=11) gTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCCCACGTTGGGGGGTACAACCTGACAACAGAACGGGGGCTAATCTTG‑GTCCAGAACAATTCAAGAATAGCCGCAAAAt > 1:599727/1‑150 (MQ=11) | TGAGTCGTAGTATCCACACCAAGCCGGCGTTATCCGGTGAGGCGCAATGTTGCGGGGGCTTTATCCCTGGTGGCATTGGTTGCTGGAAAGAGAAAACCCCCGCACGTTGAGAGGTAAAACCTGACAACAGAACGGGGGCTAATCTTGAAACCAG‑ACACTTCAAGAATAGCCGCAAAAT > NODE_4/17485‑17662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |