BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NODE_20	46,127	Δ1 bp	intergenic (+3/‑2)	EcoliAnna_02844 → / → EcoliAnna_02845	tRNA‑Val/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_20	46,126	0	T	.	100.0%	688.5 / NA	163	intergenic (+2/‑3)	EcoliAnna_02844/EcoliAnna_02845	tRNA‑Val/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (78/85); total (78/85)

Only 100 of 165 total aligned reads displayed.

ATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCG  >  NODE_20/45980‑46271

aTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACtcgg                                                                                                                                                <  1:1747508/150‑4 (MQ=255)

 tCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGTGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACtcggg                                                                                                                                               >  2:3249806/1‑146 (MQ=255)

   cTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTc                                                                                                                                            >  2:1927788/1‑150 (MQ=255)

   cTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTc                                                                                                                                            <  1:3737809/150‑1 (MQ=255)

     gCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGt                                                                                                                                          <  2:2870163/150‑1 (MQ=255)

      cACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTAGCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGtt                                                                                                                                         <  1:299856/150‑1 (MQ=255)

         gACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGc                                                                                                                                      >  1:2123527/1‑150 (MQ=255)

              accacTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGt                                                                                                                                 <  1:3103521/150‑1 (MQ=255)

               ccacTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTCAGCTCAGtt                                                                                                                                >  2:1751181/1‑150 (MQ=255)

                cacTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTg                                                                                                                               <  1:2869592/150‑1 (MQ=255)

                  cTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGt                                                                                                                             >  1:2518462/1‑150 (MQ=255)

                  cTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGt                                                                                                                             >  1:3700635/1‑150 (MQ=255)

                   tAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTa                                                                                                                            <  1:2923441/150‑1 (MQ=255)

                   tAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGAGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTACGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGtt                                                                                                                            >  2:3427826/1‑149 (MQ=255)

                    aaTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTag                                                                                                                           >  2:3020833/1‑150 (MQ=255)

                    aaTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTag                                                                                                                           <  2:2368219/150‑1 (MQ=255)

                    aaTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTag                                                                                                                           <  1:1281136/150‑1 (MQ=255)

                     aTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTaga                                                                                                                          >  2:2649701/1‑150 (MQ=255)

                          ggTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                     <  2:1230731/150‑1 (MQ=255)

                          ggTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                     <  1:182227/150‑1 (MQ=255)

                                   ggTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGCGCAGTTGACTTTTa                                                                                                            >  2:185681/1‑150 (MQ=255)

                                     tGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                          <  2:3044629/150‑1 (MQ=255)

                                     tGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCCCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                          <  1:1417799/150‑1 (MQ=255)

                                      ggAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                         >  2:1606690/1‑150 (MQ=255)

                                         agtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                      >  2:3062566/1‑150 (MQ=37)

                                         agtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                      <  2:135507/150‑1 (MQ=37)

                                         agtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                      <  1:973118/150‑1 (MQ=37)

                                         agtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAAt                                                                                                      >  2:2738534/1‑150 (MQ=255)

                                          gtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                     <  2:784977/150‑1 (MQ=25)

                                          gtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                     >  2:1782827/1‑150 (MQ=255)

                                            agtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                                                                                   >  2:4089051/1‑150 (MQ=21)

                                            agtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                                                                                   >  2:836350/1‑150 (MQ=21)

                                              tttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc                                                                                                 <  1:1946708/150‑1 (MQ=17)

                                               ttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg                                                                                                >  2:2101678/1‑150 (MQ=17)

                                                 gtagtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCa                                                                                              >  2:3954560/1‑150 (MQ=17)

                                                 gtagtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCa                                                                                              >  2:2611002/1‑150 (MQ=17)

                                                  tagtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCACCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACGCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                                                                                             <  1:2375399/150‑1 (MQ=12)

                                                     taTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                          <  2:214554/150‑1 (MQ=17)

                                                     taTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                          <  2:2039998/150‑1 (MQ=17)

                                                      aTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCCCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                         <  2:3600351/150‑1 (MQ=12)

                                                       tCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTACTCAATTGGTCGCAGGTTCg                                                                                        >  2:675206/1‑150 (MQ=12)

                                                         cAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaa                                                                                      >  2:1968694/1‑150 (MQ=17)

                                                         cAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaa                                                                                      >  2:1432640/1‑150 (MQ=17)

                                                         cAGCGCAGTATCGGGTGATTAGCTCAGCTGCGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaa                                                                                      >  1:1003639/1‑150 (MQ=12)

                                                          aGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                     <  2:1938044/150‑1 (MQ=17)

                                                          aGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                     >  2:3362211/1‑150 (MQ=17)

                                                             gcAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                                  >  1:1960357/1‑150 (MQ=14)

                                                             gcAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                                  <  2:1399720/150‑1 (MQ=14)

                                                              cAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGACGCAGGTTCGAATCCTg                                                                                 >  1:2905973/1‑150 (MQ=2)

                                                                                      gggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCa                                                         >  2:3587471/1‑150 (MQ=11)

                                                                                      gggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCa                                                         <  2:3729545/150‑1 (MQ=11)

                                                                                      gggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCa                                                         >  1:1095653/1‑150 (MQ=11)

                                                                                         agagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAAc                                                      <  1:1585404/150‑1 (MQ=17)

                                                                                          gagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACt                                                     >  2:1288768/1‑150 (MQ=18)

                                                                                           agCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTc                                                    <  1:367319/150‑1 (MQ=21)

                                                                                           agCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCACAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTc                                                    <  1:2787442/150‑1 (MQ=19)

                                                                                           agCACCTCCCCTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTc                                                    <  1:2231015/150‑1 (MQ=19)

                                                                                            gCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCa                                                   >  2:870001/1‑150 (MQ=25)

                                                                                              aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGCCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                 <  1:2834649/150‑1 (MQ=255)

                                                                                               ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                <  2:429033/150‑1 (MQ=37)

                                                                                               ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                <  1:1305989/150‑1 (MQ=37)

                                                                                                cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATGCTGCACGACCCACCAGTTTTAACATCAAACTCAGATg                                               <  1:2106666/150‑1 (MQ=255)

                                                                                                cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATg                                               <  1:2078781/150‑1 (MQ=37)

                                                                                                  cccTTACAAGGAGGGGGTCGGCGGTTGGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGtt                                             <  1:3249806/150‑1 (MQ=255)

                                                                                                  cccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGtt                                             <  2:711983/150‑1 (MQ=37)

                                                                                                   ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTa                                            >  1:3125797/1‑150 (MQ=37)

                                                                                                   ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTCAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTa                                            >  1:3342540/1‑150 (MQ=255)

                                                                                                     ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAg                                          <  1:3857087/150‑1 (MQ=37)

                                                                                                     ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAg                                          <  1:3809647/150‑1 (MQ=37)

                                                                                                     ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAg                                          <  1:3419830/150‑1 (MQ=37)

                                                                                                      tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGc                                         <  1:1458179/150‑1 (MQ=37)

                                                                                                      tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCACCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGc                                         <  2:415622/150‑1 (MQ=255)

                                                                                                         aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTg                                      >  2:832341/1‑150 (MQ=37)

                                                                                                          aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTTATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                     >  1:1862810/1‑150 (MQ=255)

                                                                                                           ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGaa                                    >  1:121907/1‑150 (MQ=255)

                                                                                                           ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGaa                                    >  2:3467395/1‑150 (MQ=255)

                                                                                                             aggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAgg                                  >  2:3891327/1‑150 (MQ=255)

                                                                                                             aggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAgg                                  >  2:2569098/1‑150 (MQ=255)

                                                                                                                gggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCTCCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATa                               <  1:3198585/150‑1 (MQ=255)

                                                                                                                gggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATa                               <  2:3323854/150‑1 (MQ=255)

                                                                                                                      gcggTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTg                         <  1:2611002/150‑1 (MQ=255)

                                                                                                                       cggTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAgtaaagtgaata                        <  2:2041862/150‑12 (MQ=14)

                                                                                                                        ggTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                       <  1:2653823/150‑1 (MQ=255)

                                                                                                                             gATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGc                  >  2:217101/1‑150 (MQ=255)

                                                                                                                             gATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGc                  >  1:677519/1‑150 (MQ=255)

                                                                                                                             gATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGc                  >  1:4103838/1‑150 (MQ=255)

                                                                                                                              aTCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCa                 >  2:140213/1‑150 (MQ=255)

                                                                                                                              aTCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCa                 >  2:299576/1‑150 (MQ=255)

                                                                                                                               tCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCaa                <  1:870001/150‑1 (MQ=255)

                                                                                                                               acccGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCaa                >  1:2625078/2‑150 (MQ=255)

                                                                                                                                cccGTCATCCCCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAAc               >  2:1607931/1‑150 (MQ=255)

                                                                                                                                  cGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACgg             <  2:2518462/150‑1 (MQ=255)

                                                                                                                                   gTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc            <  1:836350/150‑1 (MQ=255)

                                                                                                                                      atcaCCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCg         <  2:3184295/150‑1 (MQ=255)

                                                                                                                                        cacccaccAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTa       >  1:3062513/1‑150 (MQ=255)

                                                                                                                                          cccaccAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAgg     <  2:484397/150‑1 (MQ=255)

                                                                                                                                           ccaccaC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAggg    >  1:2360093/1‑150 (MQ=255)

                                                                                                                                           ccaccaC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAggg    >  2:311301/1‑150 (MQ=255)

                                                                                                                                           ccaccaC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAggg    <  1:460938/150‑1 (MQ=255)

                                                                                                                                             accac‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg  <  1:2649701/150‑1 (MQ=255)

ATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCG  >  NODE_20/45980‑46271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`