BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NODE_20	45,821	Δ2 bp	intergenic (+2/‑2)	EcoliAnna_02842 → / → EcoliAnna_02843	tRNA‑Val/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_20	45,821	0	T	.	100.0%	689.0 / NA	162	intergenic (+2/‑3)	EcoliAnna_02842/EcoliAnna_02843	tRNA‑Val/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (70/92); total (70/92)
*	NODE_20	45,822	0	T	.	99.4%	680.8 / ‑2.8	162	intergenic (+3/‑2)	EcoliAnna_02842/EcoliAnna_02843	tRNA‑Val/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (70/91); minor base G (0/1); total (70/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 162 total aligned reads displayed.

GAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGG  >  NODE_20/45680‑45963

||

gAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGtt                                                                                                                                      >  1:3100727/1‑150 (MQ=255)

 aaGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTa                                                                                                                                     <  1:2378875/150‑1 (MQ=255)

   gacGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGc                                                                                                                                   <  2:680482/148‑1 (MQ=255)

   gTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTCCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGc                                                                                                                                   <  1:906965/150‑1 (MQ=255)

      gAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCa                                                                                                                                >  2:3142776/1‑150 (MQ=255)

        gTCATCCTGCCCGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCACCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGt                                                                                                                              <  2:3112695/150‑1 (MQ=255)

        gTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAgc                                                                                                                              >  2:2248202/1‑149 (MQ=255)

         tCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGtt                                                                                                                             <  2:3809614/150‑1 (MQ=255)

          cATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTg                                                                                                                            >  2:299856/1‑150 (MQ=255)

            tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGt                                                                                                                          <  2:2171179/150‑1 (MQ=255)

            tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGt                                                                                                                          >  2:122665/1‑150 (MQ=255)

             ccTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTa                                                                                                                         >  2:1253015/1‑150 (MQ=255)

              cTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTag                                                                                                                        <  2:1990300/150‑1 (MQ=255)

              cTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGCTCGTTAGCTCAGTTGGTag                                                                                                                        <  1:3752452/150‑1 (MQ=255)

                gCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTagag                                                                                                                      <  1:4126601/150‑1 (MQ=255)

                gCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTagag                                                                                                                      >  1:792285/1‑150 (MQ=255)

                 cACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGc                                                                                                                     <  1:239240/150‑1 (MQ=255)

                 cACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGc                                                                                                                     <  1:3042969/150‑1 (MQ=255)

                    gACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                  <  2:2429018/150‑1 (MQ=255)

                     aCCCACCACTAACATAGTTAGTTGTAGTATCCAGGGTAGAATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                                                                                 <  1:3826406/150‑1 (MQ=255)

                     aCCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                                                                                 <  2:3294768/150‑1 (MQ=255)

                      cccACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                                                                                                <  1:1784607/150‑1 (MQ=255)

                      cccACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                                                                                                <  2:1986334/150‑1 (MQ=255)

                      cccACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTGAGCTCAGTTGGTAGAGCAGTTg                                                                                                                >  1:4083949/1‑150 (MQ=255)

                        caccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc                                                                                                              <  1:2812529/150‑1 (MQ=255)

                          ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                                                            >  1:3486700/1‑150 (MQ=255)

                          ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                                                            >  2:3359573/1‑150 (MQ=255)

                          ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                                                            <  1:3086163/150‑1 (MQ=255)

                          ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                                                            >  2:2782442/1‑150 (MQ=255)

                          ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                                                            >  1:2368219/1‑150 (MQ=255)

                           cacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                           <  2:1185320/150‑1 (MQ=255)

                           cacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtta                                                                                                           <  1:3317227/150‑2 (MQ=255)

                           cacTAACATAGTTAGTTGTAGTACCCAGCGTAGTATCGGGTGATTAGGTCAGCTGGGAGAGCACCCCCCTTACAAGGAGGGGGTCGGCGGATCGATCCCGTCATCACCCACCAC‑‑CGGGCCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                           <  1:3029389/150‑1 (MQ=14)

                            attAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtttt                                                                                                          <  1:2164362/148‑1 (MQ=255)

                            acTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtttt                                                                                                          <  1:1681352/150‑1 (MQ=255)

                             cTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCCCAGTTGGTAGAGCAGTTGACTTTTa                                                                                                         <  2:3234784/150‑1 (MQ=255)

                              tAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                                                        <  1:3551790/150‑1 (MQ=255)

                               aaCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTAAGCTCAGTTGGTAGAGCAGTTGTCTTTTAAt                                                                                                       >  2:367319/1‑150 (MQ=14)

                                 cATAGTTAGTTGTAGTATCCGGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCa                                                                                                     >  2:2777776/1‑150 (MQ=255)

                                  aTAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGAGGGTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                    >  2:899802/1‑150 (MQ=255)

                                    agttagttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                  <  2:1132665/150‑1 (MQ=255)

                                     gttagttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTg                                                                                                 <  2:1283503/150‑1 (MQ=255)

                                      ttagttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                                                                                >  2:2596912/1‑150 (MQ=255)

                                       tagttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                                                                               <  1:4108204/150‑1 (MQ=19)

                                        agttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc                                                                                              <  1:2501073/150‑1 (MQ=16)

                                         gttTTATTACCCAGCGTAGTAACGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg                                                                                             <  2:4088026/150‑1 (MQ=11)

                                          ttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCAC‑‑CGGGGCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGc                                                                                            >  2:2526916/1‑150 (MQ=255)

                                               gtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                       >  1:3328990/1‑150 (MQ=12)

                                               gtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                       >  1:1500617/1‑150 (MQ=12)

                                               gtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                       <  2:2668584/150‑1 (MQ=12)

                                                taTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                      <  1:247117/150‑1 (MQ=12)

                                                 aTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                                                                                     <  2:2174781/150‑1 (MQ=12)

                                                 aTCCAGCGTACTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGCCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                                                                                     <  1:2269706/150‑1 (MQ=255)

                                                   ccAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaa                                                                                   >  1:2932504/1‑150 (MQ=12)

                                                      gCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                                                                                >  2:1717085/1‑150 (MQ=12)

                                                       cGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                               <  1:238962/150‑1 (MQ=12)

                                                        gtagtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTg                                                                              >  2:3635107/1‑150 (MQ=9)

                                                        gtagtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTg                                                                              >  2:2869592/1‑150 (MQ=9)

                                                                                tGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCg                                                      >  2:2404243/1‑150 (MQ=2)

                                                                                 gggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGCGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGa                                                     >  2:1936451/1‑150 (MQ=9)

                                                                                 gggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGa                                                     >  1:714382/1‑150 (MQ=9)

                                                                                  ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGaa                                                    <  2:3056020/150‑1 (MQ=12)

                                                                                   gagaGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAg                                                   >  2:662130/1‑150 (MQ=12)

                                                                                    agagCCCCTCCCTTACAAGGAGGGGGCCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCGCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGa                                                  <  2:1358336/150‑1 (MQ=11)

                                                                                     gagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCCCGACCCACCAGTTTTAACATCGAAGAc                                                 <  2:2383811/150‑1 (MQ=255)

                                                                                     gagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGAc                                                 >  1:1583144/1‑150 (MQ=19)

                                                                                         aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGAt                                             >  2:3198585/1‑150 (MQ=255)

                                                                                           cTCCCTTACAAGGAGGGGGCCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGt                                           <  1:1022966/150‑1 (MQ=255)

                                                                                             cccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTGATCACCCACCA‑‑GCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTa                                         <  1:3947390/150‑1 (MQ=255)

                                                                                             cccTTACAAGGAGGGGGACGGCGGTTCGATCCCGTCATCACTCAGCAC‑‑CGGGTCGTTAGCACAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTa                                         <  2:215200/150‑1 (MQ=11)

                                                                                                ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGc                                      <  2:3129474/150‑1 (MQ=255)

                                                                                                 tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCg                                     <  2:1124492/150‑1 (MQ=255)

                                                                                                    aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCgtgt                                  >  1:347465/1‑150 (MQ=255)

                                                                                                      ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAg                                <  2:2017514/150‑1 (MQ=255)

                                                                                                          ggggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATa                            <  1:1592393/150‑1 (MQ=255)

                                                                                                           gggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTAAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGTAGACAGATGTTAAGCGTGGAGGATag                           >  2:1326309/1‑149 (MQ=11)

                                                                                                            ggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAAc                          >  2:569374/1‑150 (MQ=255)

                                                                                                            ggTCGGCGGCTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGAGAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTAGAAACCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGGTAAGAGAGTAGGATaag                          >  2:2124629/1‑149 (MQ=14)

                                                                                                              tCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGt                        >  1:245923/1‑150 (MQ=255)

                                                                                                              tCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGt                        >  1:891161/1‑150 (MQ=255)

                                                                                                               cggcggTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTGCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGtt                       <  2:1829350/150‑1 (MQ=255)

                                                                                                                ggcggTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTg                      <  2:3101800/150‑1 (MQ=255)

                                                                                                                ggcggTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTg                      <  1:1817794/150‑1 (MQ=255)

                                                                                                                      tCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAg                <  1:439112/150‑1 (MQ=255)

                                                                                                                        gATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCa              >  2:411529/1‑150 (MQ=255)

                                                                                                                         aTCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCaa             >  1:214554/1‑150 (MQ=255)

                                                                                                                         aTCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACAACGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCaa             <  1:3487265/150‑1 (MQ=255)

                                                                                                                          tCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAAc            <  2:4038478/150‑1 (MQ=255)

                                                                                                                            ccGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACgg          >  1:1077886/1‑150 (MQ=255)

                                                                                                                             cGTCACCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGc         <  1:167140/150‑1 (MQ=255)

                                                                                                                              gTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGcc        <  2:2313201/150‑1 (MQ=255)

                                                                                                                              gTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGcc        >  1:2414787/1‑150 (MQ=255)

                                                                                                                              gTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGcc        <  2:4106364/150‑1 (MQ=255)

                                                                                                                                catcaCCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCg      >  2:2911334/1‑150 (MQ=255)

                                                                                                                                catcaCCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCg      <  2:1751787/150‑1 (MQ=255)

                                                                                                                                   cacccaccAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAg   >  1:3207721/1‑150 (MQ=255)

                                                                                                                                   cacccaccAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAg   <  1:699315/150‑1 (MQ=255)

                                                                                                                                   cacccaccAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGCTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGTTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGGAg   >  2:3560802/1‑150 (MQ=14)

                                                                                                                                    acccaccAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAgg  <  2:2853533/150‑1 (MQ=255)

                                                                                                                                    acccaccAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAgg  <  1:1883515/150‑1 (MQ=255)

||

GAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGG  >  NODE_20/45680‑45963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`