Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NODE_954890AT68.0% 606.0 / inf 426intergenic (‑54/–)EcoliAnna_04303/–tRNA‑Ile/–
Reads supporting (aligned to +/- strand):  ref base A (48/87);  new base T (120/167);  total (172/254)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 430 total aligned reads displayed.

CAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NODE_95/340‑566
                                                                                                                                                     |                                                                                            
aaaaTTTGCCCTGCAAATTTGGTAGGCCTGCGTGGACTTCAACCACCGCCCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAGGa                                                                                              >  2:1981423/2‑150 (MQ=255)
  aaTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGTCCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGATTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAAc                                                                                            >  1:558733/1‑150 (MQ=255)
  aaTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTAc                                                                                            <  2:3853737/150‑1 (MQ=255)
   aTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGGCCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACg                                                                                           >  1:1126808/1‑150 (MQ=255)
   aTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACg                                                                                           <  2:2210647/150‑1 (MQ=255)
    tttGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGc                                                                                          <  1:2149548/150‑1 (MQ=255)
      tGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGctt                                                                                        <  1:3617764/150‑1 (MQ=255)
       gCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttc                                                                                       <  1:3324326/150‑1 (MQ=255)
        ccGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct                                                                                      <  1:2809597/150‑1 (MQ=255)
        ccGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct                                                                                      <  1:91554/150‑1 (MQ=255)
        ccGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct                                                                                      <  2:1050037/150‑1 (MQ=255)
        ccGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGAAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct                                                                                      >  2:1482709/1‑150 (MQ=255)
         cGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttctt                                                                                     >  1:130754/1‑150 (MQ=255)
          gTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCttt                                                                                    >  2:646450/1‑150 (MQ=255)
          gTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCttt                                                                                    <  1:4024175/150‑1 (MQ=255)
          gTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCttt                                                                                    >  1:3534944/1‑150 (MQ=255)
            gCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaa                                                                                  <  2:2372285/150‑1 (MQ=255)
              aaaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTtaagg                                                                                >  2:1794308/1‑150 (MQ=255)
               aaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTtaaggt                                                                               <  2:1130181/150‑1 (MQ=255)
               aaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaaggt                                                                               <  1:108232/150‑1 (MQ=255)
               aaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaaggt                                                                               <  2:3196830/150‑1 (MQ=255)
                 tttGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaagttaa                                                                             <  2:3602530/150‑1 (MQ=255)
                    ggTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAagga                                                                          >  1:692786/1‑150 (MQ=255)
                    ggTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAagga                                                                          <  1:4122346/150‑1 (MQ=255)
                    ggTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCAACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAagga                                                                          <  1:2787191/150‑1 (MQ=255)
                     gTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAaggag                                                                         >  2:2618570/1‑150 (MQ=255)
                     gTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAaggag                                                                         <  1:3916844/150‑1 (MQ=255)
                      tAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAaggagg                                                                        >  1:2737166/1‑150 (MQ=255)
                           cTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATc                                                                   <  2:1432698/150‑1 (MQ=255)
                           cTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATc                                                                   <  2:929229/150‑1 (MQ=255)
                            tGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATcc                                                                  >  2:896309/1‑150 (MQ=255)
                            tGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATcc                                                                  <  2:3353232/150‑1 (MQ=255)
                               gTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAAc                                                               <  1:871490/150‑1 (MQ=255)
                               gTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAAc                                                               <  1:1689178/150‑1 (MQ=255)
                                 ggACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCg                                                             <  1:4065496/150‑1 (MQ=255)
                                     ttGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAgg                                                         <  2:2100462/150‑1 (MQ=255)
                                       gAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGGACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGtt                                                       >  1:2005808/1‑150 (MQ=255)
                                       gAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGtt                                                       <  2:4106171/150‑1 (MQ=255)
                                       gAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGtt                                                       <  1:383539/150‑1 (MQ=255)
                                       gAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGtt                                                       <  1:1787738/150‑1 (MQ=255)
                                       gAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGtt                                                       <  2:3027225/150‑1 (MQ=255)
                                         accaccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCaaagtcg                                                     >  2:2234989/1‑144 (MQ=255)
                                          ccaccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTccc                                                    <  1:2237556/150‑1 (MQ=255)
                                          ccaccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTccc                                                    >  1:266678/1‑150 (MQ=255)
                                           caccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTcccc                                                   <  1:2519039/150‑1 (MQ=255)
                                            acttaCCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCt                                                  <  1:3450922/146‑1 (MQ=255)
                                             ccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTa                                                 <  1:1291972/150‑1 (MQ=255)
                                             ccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTa                                                 <  2:2501894/150‑1 (MQ=255)
                                              cGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTAc                                                <  2:2861727/150‑1 (MQ=255)
                                                aCCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACgg                                              >  2:2040837/1‑150 (MQ=255)
                                                 ccTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGt                                             <  1:2389423/150‑1 (MQ=255)
                                                  cTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGtt                                            <  2:3644217/150‑1 (MQ=255)
                                                  cTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGACGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGCGATCCAACCGCAGGTTCCCCTACGGtt                                            >  1:2312495/1‑150 (MQ=255)
                                                   tCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTa                                           >  2:3523327/1‑150 (MQ=255)
                                                     tcccGTAGCAGGGGTGCGCTCTAACGACCTGAGCTTCAAGCCTGTAGAGGTTTTACTGCTCATTTCCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCGAACCGCAGGTTCCCCTACGGTTAcc                                         <  2:3987693/149‑1 (MQ=37)
                                                       ccTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCtt                                       <  2:3283960/150‑1 (MQ=255)
                                                       ccTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCtt                                       <  2:2772440/150‑1 (MQ=255)
                                                        cTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTg                                      >  1:3044186/1‑150 (MQ=255)
                                                        cTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTg                                      <  1:529787/150‑1 (MQ=255)
                                                          tATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGtt                                    >  1:1843693/1‑150 (MQ=255)
                                                          tATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGtt                                    <  1:155780/150‑1 (MQ=255)
                                                            tCAGGGGTGCGCTCTAACCACCTGAGGTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTAc                                  <  1:1246948/150‑1 (MQ=255)
                                                            tCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTAc                                  <  1:97952/150‑1 (MQ=255)
                                                            tCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCATCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTAc                                  <  1:1485479/150‑1 (MQ=255)
                                                              aGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGa                                <  1:2035940/150‑1 (MQ=255)
                                                                gggTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACTATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACt                              >  1:779444/1‑150 (MQ=255)
                                                                gggTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACt                              >  1:2983781/1‑150 (MQ=255)
                                                                    gcgcTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGTTTCTTTAAGTTAAGAAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACATCAc                          >  1:2778342/1‑150 (MQ=37)
                                                                    gcgcTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCAc                          >  1:2178870/1‑150 (MQ=255)
                                                                       ctctAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCAcccc                       <  1:311776/150‑1 (MQ=255)
                                                                        tctAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCa                      <  2:1166006/150‑1 (MQ=255)
                                                                          tAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGt                    <  1:1927573/150‑1 (MQ=255)
                                                                          tAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGt                    >  1:3567732/1‑150 (MQ=255)
                                                                          tAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGt                    <  2:692190/150‑1 (MQ=255)
                                                                           aaCCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTc                   <  2:2346516/150‑1 (MQ=255)
                                                                            accaccTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCa                  <  1:3764726/150‑1 (MQ=255)
                                                                             ccaccTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAt                 <  2:3712492/150‑1 (MQ=255)
                                                                              caccTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGACGTTATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtg                >  1:2452869/1‑149 (MQ=255)
                                                                                  tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatc            <  1:3237256/150‑6 (MQ=255)
                                                                                  tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatc            <  1:1430639/150‑6 (MQ=255)
                                                                                  tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatc            <  1:1014927/150‑6 (MQ=255)
                                                                                  tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatc            <  1:3996141/150‑6 (MQ=255)
                                                                                  tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGGCTTCACCCCAGTCAtgaatc            >  1:44468/1‑145 (MQ=255)
                                                                                  tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTACATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatc            <  2:1705693/150‑6 (MQ=255)
                                                                                    aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGTCAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcac          >  1:2610902/1‑143 (MQ=255)
                                                                                    aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcac          <  2:1315613/150‑8 (MQ=255)
                                                                                    aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcac          >  2:3952513/1‑143 (MQ=255)
                                                                                    aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcac          <  2:482002/150‑8 (MQ=255)
                                                                                     gCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcaca         >  1:1726323/1‑142 (MQ=255)
                                                                                      cTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGTGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaa        <  2:86153/150‑10 (MQ=21)
                                                                                       tACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaa       <  2:1752875/150‑11 (MQ=255)
                                                                                       tACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaa       <  1:4080461/150‑11 (MQ=255)
                                                                                       tACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaa       <  2:157978/150‑11 (MQ=21)
                                                                                        aCAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaag      <  1:621423/150‑12 (MQ=18)
                                                                                          aaGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtg    <  2:2648057/150‑14 (MQ=18)
                                                                                          aaGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtg    >  1:3374743/1‑137 (MQ=18)
                                                                                          aaGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtg    <  2:3127303/150‑14 (MQ=18)
                                                                                           aGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtgg   <  2:3400545/150‑15 (MQ=255)
                                                                                            gCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtggt  >  2:3080984/1‑135 (MQ=18)
                                                                                            gCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtggt  <  1:1377930/150‑16 (MQ=18)
                                                                                                                                                     |                                                                                            
CAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NODE_95/340‑566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑