Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NODE_954830TG67.6% 671.9 / inf 453intergenic (‑48/–)EcoliAnna_04303/–tRNA‑Ile/–
Reads supporting (aligned to +/- strand):  ref base T (55/91);  new base G (128/177);  total (184/269)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 456 total aligned reads displayed.

CCTCTTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NODE_95/334‑566
                                                                                                                                                     |                                                                                                  
  tctTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCa                                                                                                  >  2:349857/1‑150 (MQ=255)
  tctTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCa                                                                                                  >  2:51590/1‑150 (MQ=255)
   ctgcAAATTTGCCGTGCAGATTTGGTAGGCCTGAGTGGACTTGAACGACCGACCTCACACTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCaa                                                                                                 <  1:778677/147‑1 (MQ=255)
    ttCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCaaa                                                                                                <  1:3075704/150‑1 (MQ=255)
    ttCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCaaa                                                                                                <  1:3214767/150‑1 (MQ=255)
        aaTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTAc                                                                                            <  2:2811913/150‑1 (MQ=255)
        aaTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTAc                                                                                            <  2:2510412/150‑1 (MQ=255)
         aTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACg                                                                                           <  2:2210647/150‑1 (MQ=255)
         aTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACg                                                                                           >  2:1904155/1‑150 (MQ=255)
         aTTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACg                                                                                           >  1:3752530/1‑150 (MQ=255)
          tttGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGc                                                                                          <  1:2149548/150‑1 (MQ=255)
          tttGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGc                                                                                          >  1:2898336/1‑150 (MQ=255)
             gCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttc                                                                                       <  1:3998483/150‑1 (MQ=255)
             gCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttc                                                                                       <  1:3770705/150‑1 (MQ=255)
             gCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttc                                                                                       <  1:3324326/150‑1 (MQ=255)
              ccGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct                                                                                      <  1:2506360/150‑1 (MQ=255)
              ccGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGAAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGcttct                                                                                      >  2:1482709/1‑150 (MQ=255)
                gTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCttt                                                                                    <  2:3632144/150‑1 (MQ=255)
                gTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCttt                                                                                    >  1:3534944/1‑150 (MQ=255)
                gTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCttt                                                                                    >  2:32526/1‑150 (MQ=255)
                 tGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATTAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTta                                                                                   >  1:1431145/1‑150 (MQ=255)
                 tGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTta                                                                                   >  1:3036018/1‑150 (MQ=255)
                 tGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTta                                                                                   <  1:1250127/150‑1 (MQ=255)
                    aaaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTtaagg                                                                                >  2:1794308/1‑150 (MQ=255)
                     aaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTtaaggt                                                                               <  2:1130181/150‑1 (MQ=255)
                     aaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaaggt                                                                               <  1:108232/150‑1 (MQ=255)
                      aTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaaggta                                                                              <  1:1975604/150‑1 (MQ=255)
                        ttGGTAGGCCTGAGTGGACTTCAACCACCGACCTCACTCTTTTCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTAGTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaaggtaag                                                                            <  2:2632714/150‑1 (MQ=255)
                         tGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTACCCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTtaaggtaagg                                                                           <  1:2613341/150‑1 (MQ=255)
                          ggTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAagga                                                                          >  1:692786/1‑150 (MQ=255)
                           gTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAaggag                                                                         >  1:4131855/1‑150 (MQ=255)
                           gTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAaggag                                                                         <  1:1547121/150‑1 (MQ=255)
                            tAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATTAGACAATCTGTGTGAGCACTTCAAAGAACGCTACTTTAAGGTAaggagg                                                                        <  1:554403/150‑1 (MQ=255)
                             ttgCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGt                                                                       <  2:1688724/148‑1 (MQ=255)
                             aGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGt                                                                       <  1:2182840/150‑1 (MQ=255)
                             aGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGt                                                                       <  1:519103/150‑1 (MQ=255)
                              ggCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTg                                                                      >  2:1189707/1‑150 (MQ=255)
                              ggCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTg                                                                      <  1:912960/150‑1 (MQ=255)
                                ccTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGAt                                                                    >  1:1649891/1‑150 (MQ=255)
                                ccTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCACTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGAt                                                                    <  1:270802/150‑1 (MQ=255)
                                 cTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATc                                                                   <  2:1432698/150‑1 (MQ=255)
                                 cTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATc                                                                   >  1:2407342/1‑150 (MQ=255)
                                  tGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATcc                                                                  <  2:2347822/150‑1 (MQ=255)
                                  tGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATcc                                                                  >  2:896309/1‑150 (MQ=255)
                                  tGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATcc                                                                  >  1:2783541/1‑150 (MQ=255)
                                  tGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATcc                                                                  <  2:3093357/150‑1 (MQ=255)
                                    aGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGTGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCaa                                                                >  1:180393/1‑150 (MQ=255)
                                    aGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCaa                                                                >  2:647792/1‑150 (MQ=255)
                                     gTGGTCTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGGCTGTAGAGGTTTTACTGCTGATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAAc                                                               <  2:1594605/150‑1 (MQ=255)
                                      tGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCACATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAAcc                                                              <  1:2798904/150‑1 (MQ=255)
                                       ggACTTGGACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCACCCg                                                             >  1:404296/1‑150 (MQ=255)
                                       ggACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCg                                                             >  1:1294272/1‑150 (MQ=255)
                                         aCTTGAACCACCGACCACAACCTTATCTGGGGTGCGCTCTAACCACCTGAGATACAACCCTGTAGAGGATTTACTCCTCATTTTCATCAGACAATCTGAGTGTCCACTTCAAAGACCGCTTCTTTACGGTAAGCAGGTGATCCAACCCCa                                                           >  2:3594595/1‑150 (MQ=255)
                                           ttGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAgg                                                         <  2:2100462/150‑1 (MQ=255)
                                           ttGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAgg                                                         <  2:418390/150‑1 (MQ=255)
                                             gAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGGTCCAACCGCAGGtt                                                       >  1:1314206/1‑150 (MQ=255)
                                               accaccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCaaagtcg                                                     >  2:2234989/1‑144 (MQ=255)
                                                ccaccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTccc                                                    <  2:376865/150‑1 (MQ=255)
                                                  accGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCt                                                  >  2:2387935/1‑150 (MQ=255)
                                                   ccGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTa                                                 <  2:3076204/150‑1 (MQ=255)
                                                    cGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGGTCCAACCGCAGGTTCCCCTAc                                                >  1:2315976/1‑150 (MQ=255)
                                                    cGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTAc                                                >  1:1136940/1‑150 (MQ=255)
                                                      aCCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACgg                                              >  2:2040837/1‑150 (MQ=255)
                                                        cTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGtt                                            <  2:3323804/150‑1 (MQ=255)
                                                          cACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACATGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTAc                                          >  1:4113075/1‑150 (MQ=255)
                                                           aCCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTAcc                                         <  2:694897/150‑1 (MQ=255)
                                                            cccTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCACAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCt                                        <  2:1294272/150‑1 (MQ=255)
                                                              cTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGGTTTACTGCTCATTTTCATCAGACAATCTGTGTGTGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTg                                      >  1:855699/1‑150 (MQ=255)
                                                                 aTCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTa                                   <  2:2958694/150‑1 (MQ=255)
                                                                  tCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTAc                                  <  1:97952/150‑1 (MQ=255)
                                                                  tCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTAc                                  <  1:3469275/150‑1 (MQ=255)
                                                                     ggggTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGAc                               <  1:1884080/150‑1 (MQ=255)
                                                                       ggTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACtt                             <  2:3943957/150‑1 (MQ=255)
                                                                        gTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTc                            <  1:3637382/150‑1 (MQ=255)
                                                                            gcTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCAccc                        >  2:4050774/1‑150 (MQ=255)
                                                                             ctctAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCAcccc                       <  1:1775705/150‑1 (MQ=255)
                                                                             ctctAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCAcccc                       <  2:3956527/150‑1 (MQ=255)
                                                                              tctAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCa                      <  1:3975171/150‑1 (MQ=255)
                                                                               ctAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAg                     <  1:2753723/150‑1 (MQ=255)
                                                                                 aaCCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGATACCTTGTTACGACTTCACCCCAGTc                   <  2:940473/150‑1 (MQ=255)
                                                                                 aaCCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTc                   <  2:3164733/150‑1 (MQ=255)
                                                                                 aaCCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTc                   <  1:3848752/150‑1 (MQ=255)
                                                                                 aaCCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTc                   <  2:1037557/150‑1 (MQ=255)
                                                                                   ccaccTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAt                 <  1:2258327/150‑1 (MQ=255)
                                                                                   ccaccTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAt                 <  2:3712492/150‑1 (MQ=255)
                                                                                    caccTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtg                >  1:3244728/1‑149 (MQ=255)
                                                                                     accTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtga               <  2:2742569/150‑3 (MQ=255)
                                                                                     accTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtga               >  1:1414077/1‑148 (MQ=255)
                                                                                       cTGAGCTACAAGCCTGTAGAGGTATTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGttgtgaat             >  1:2016574/1‑143 (MQ=255)
                                                                                        tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatc            <  1:575784/150‑6 (MQ=255)
                                                                                        tGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatc            <  1:1014927/150‑6 (MQ=255)
                                                                                          aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcac          >  1:2558802/1‑143 (MQ=255)
                                                                                          aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGTGGTGTTCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcac          >  1:407598/1‑143 (MQ=21)
                                                                                          aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcac          >  1:2062616/1‑143 (MQ=255)
                                                                                            cTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGTGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaa        <  2:86153/150‑10 (MQ=21)
                                                                                             tACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaa       <  2:1752875/150‑11 (MQ=255)
                                                                                                aaGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtg    >  1:3587855/1‑137 (MQ=18)
                                                                                                aaGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtg    >  1:3374743/1‑137 (MQ=18)
                                                                                                 aGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtgaatcacaaagtgg   <  2:3400545/150‑15 (MQ=255)
                                                                                                  gCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAtggatcacaaagtggt  >  1:1708716/1‑135 (MQ=18)
                                                                                                                                                     |                                                                                                  
CCTCTTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NODE_95/334‑566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑