BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_92	136	0	G	A	66.3%	267.4 / inf	520	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (95/80); new base A (169/175); total (265/255)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 523 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑CACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGT  >  NODE_92/1‑284

acccactggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTAt                                                                                                                                                 <  1:1176680/141‑1 (MQ=21)

acccactggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTAt                                                                                                                                                 <  1:1466930/141‑1 (MQ=21)

    actggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACa                                                                                                                                             >  2:1674954/6‑150 (MQ=255)

    actggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatg                                                                                                                                             >  2:3364181/6‑148 (MQ=18)

       ggcACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgaga                                                                                                                                          >  1:3137378/3‑145 (MQ=21)

        gcACTATGACGAAGCAGACCGCCACACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTaa                                                                                                                                         <  2:3591556/149‑1 (MQ=255)

          aCTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAg                                                                                                                                       >  1:2528119/1‑150 (MQ=255)

           cTATGACGAAGCAGACCGCCTCACGCACCGCCCCGTGAATGACGAAACCGCAGAGCGGTGGCAGTATGACGAATGCGGCTGGCTGACAGACATCAGCCATAACAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAgg                                                                                                                                      <  2:3048207/150‑1 (MQ=37)

           cTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGAAGGTGCACTATGGTTATGACAGTtaaag                                                                                                                                      >  1:3048207/1‑148 (MQ=255)

             aTGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaaggcc                                                                                                                                    <  2:2384636/150‑12 (MQ=21)

             aTGACGAACCAGACCGCCTCACGCACCGCACGGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACAACAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGatgagaaaggcc                                                                                                                                    <  2:1127406/150‑12 (MQ=18)

               gACGAAGCAGACCGCCTCACGCACCGCACCGAGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGgccgc                                                                                                                                  >  1:1665227/1‑150 (MQ=255)

                 cGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCt                                                                                                                                <  2:1879970/150‑1 (MQ=21)

                    aGCAGACCGCCTCACGCACCGCACCGTGAATGGCGAAACCGCAGCGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCACTATGGTTATGACCGTAAAGGCCGCCTCgg                                                                                                                             >  2:1214868/1‑149 (MQ=25)

                     gCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtgacc                                                                                                                            <  2:277587/150‑5 (MQ=21)

                       aGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGGGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCtgaccgg                                                                                                                          >  2:140076/1‑144 (MQ=18)

                         aCCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGGccgggg                                                                                                                        >  2:2919583/1‑146 (MQ=21)

                              cTCACGCACCGCACCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAacac                                                                                                                   >  1:3178088/1‑150 (MQ=255)

                                  cgcaccgcacCGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACAccaag                                                                                                               >  2:3650137/1‑147 (MQ=255)

                                   gcaccgcaccGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGAc                                                                                                              <  1:1894476/150‑1 (MQ=255)

                                   gcaccgcaccGTGAATGGCGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGAc                                                                                                              <  2:691141/150‑1 (MQ=255)

                                     accgcaccgTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGagcgtcagacgg                                                                                                            >  2:1640355/1‑139 (MQ=18)

                                         caccgTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGca                                                                                                        <  2:593774/150‑1 (MQ=18)

                                           ccgTGAAGGGTGAAACCGCAGAGCGGTGGCAGCATGACGAACGTGGCTGGCTGACAGACAACAGCCACATCAGCGAAGGGCACCGGGTGGCGGTGCACTACAGGTATGATGAGAGAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatc                                                                                                      <  2:897791/150‑1 (MQ=19)

                                            cgTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatca                                                                                                     <  1:541896/150‑1 (MQ=18)

                                             gTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatcac                                                                                                    <  2:1098584/150‑2 (MQ=18)

                                              tGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGcatcacc                                                                                                   >  2:156940/1‑148 (MQ=18)

                                                     cgAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGTCAGACATCAGCCATATCTGCGAAGGGCACCCGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGTCAGTGTACACCTGACGGTGCATCATCCGCAGAc                                                                                            >  1:2691910/2‑150 (MQ=255)

                                                       aaaCCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACgg                                                                                          >  2:1499080/1‑149 (MQ=12)

                                                         aCCGCAGAGCGGTGGGAGTATGATGAACGTGGCTGGCTGACACACATCAGCCATATCAGCGAAGGGCACCAGGTGGCGGTGCATTACCGGTATGATGAGAAAGGCCGGCTGACCAGTGAGCGACAGGCGGGGCTTCACCCGCAGAcagaa                                                                                        >  1:424842/1‑146 (MQ=255)

                                                          ccGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACggaag                                                                                       <  1:616268/150‑5 (MQ=12)

                                                           cGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCCGGTGGCGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGTCGAATGa                                                                                      >  1:773641/1‑150 (MQ=255)

                                                            gCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACggaagca                                                                                     >  1:1705951/1‑144 (MQ=12)

                                                              agagCGGTGGTAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACggaagcact                                                                                   >  1:3504596/1‑142 (MQ=12)

                                                              agagCGGTGGCAGTATGAGCAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACt                                                                                   <  2:2343118/150‑1 (MQ=255)

                                                                 gCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGct                                                                                >  1:3574192/1‑150 (MQ=255)

                                                                   ggTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGGACTGctct                                                                              >  2:2979673/1‑150 (MQ=255)

                                                                   ggTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGctct                                                                              <  2:1327799/150‑1 (MQ=255)

                                                                    gTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTg                                                                             >  2:3890908/1‑150 (MQ=12)

                                                                     tGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTgg                                                                            <  2:861826/150‑1 (MQ=12)

                                                                     tGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTgg                                                                            <  2:2528119/150‑1 (MQ=255)

                                                                           tATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCAt                                                                      <  1:4089253/150‑1 (MQ=12)

                                                                           tATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCAt                                                                      <  1:3690133/150‑1 (MQ=12)

                                                                           tATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCAt                                                                      <  1:362166/150‑1 (MQ=12)

                                                                              gACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATgag                                                                   >  1:1205084/1‑150 (MQ=255)

                                                                               aCGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATgaga                                                                  >  2:285006/1‑150 (MQ=12)

                                                                               aCGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATgaga                                                                  <  1:1112632/150‑1 (MQ=12)

                                                                                 gAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGAcc                                                                <  2:729641/150‑1 (MQ=9)

                                                                                   aCGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAg                                                              <  2:2604319/150‑1 (MQ=9)

                                                                                      tggctggctGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACa                                                           <  2:327715/149‑1 (MQ=12)

                                                                                       ggctggctGACAGACCTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCAGTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGGCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACAt                                                          <  2:2436938/150‑1 (MQ=255)

                                                                                       ggctggctGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACAt                                                          <  1:2730399/150‑1 (MQ=255)

                                                                                        gctggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATg                                                         >  1:213405/1‑150 (MQ=12)

                                                                                         ctggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACAAGc                                                        >  2:1789845/1‑150 (MQ=255)

                                                                                          tggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGTGCGTCAGACGGTGTATCACCCGCAGACCGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCg                                                       >  1:3876427/1‑150 (MQ=12)

                                                                                          tggctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCg                                                       <  1:205700/150‑1 (MQ=255)

                                                                                            gctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTa                                                     >  2:3171113/1‑150 (MQ=255)

                                                                                             ctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTAc                                                    >  2:3822030/1‑150 (MQ=255)

                                                                                             ctgACAGACACCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTAc                                                    <  2:2083495/150‑1 (MQ=255)

                                                                                              tgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACa                                                   >  2:1939590/1‑150 (MQ=255)

                                                                                               gacagacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACaa                                                  >  1:327294/1‑150 (MQ=255)

                                                                                               gacagacaTCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACaa                                                  >  1:2901925/1‑150 (MQ=255)

                                                                                                acagacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAAc                                                 <  2:412677/150‑1 (MQ=255)

                                                                                                acagacaTCAGCCATACCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGCCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAAc                                                 <  2:2023693/150‑1 (MQ=255)

                                                                                                 cagacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCAGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACg                                                <  2:2741939/150‑1 (MQ=255)

                                                                                                  agacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGc                                               >  1:3991661/1‑150 (MQ=255)

                                                                                                   gacaTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGcg                                              >  1:2974096/1‑149 (MQ=255)

                                                                                                   gacaTCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGca                                              >  2:2288851/1‑150 (MQ=255)

                                                                                                     caTCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGcgca                                            >  2:3461618/1‑150 (MQ=255)

                                                                                                      aTCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAg                                           >  1:1773165/1‑150 (MQ=255)

                                                                                                        cAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAggg                                         <  1:473336/150‑1 (MQ=255)

                                                                                                         aGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAgggg                                        >  1:2608925/1‑149 (MQ=255)

                                                                                                           ccATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCt                                      <  2:3415860/150‑1 (MQ=255)

                                                                                                            cATATCACCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTg                                     <  2:3576504/150‑1 (MQ=255)

                                                                                                             atatCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTgg                                    <  1:1552482/150‑1 (MQ=255)

                                                                                                             atatCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTgg                                    >  1:2353748/1‑150 (MQ=255)

                                                                                                              tatCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGc                                   <  2:1826176/150‑1 (MQ=255)

                                                                                                              taccAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGc                                   <  2:3963557/147‑1 (MQ=255)

                                                                                                                  aGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGTTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAAc                               >  1:2204398/1‑150 (MQ=255)

                                                                                                                  aGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAAc                               >  1:1421725/1‑150 (MQ=255)

                                                                                                                   gCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAAcc                              <  2:312436/150‑1 (MQ=255)

                                                                                                                   gCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCAGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAAcc                              <  1:1356156/150‑1 (MQ=255)

                                                                                                                    cGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGGCAGACGGTGCACCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACAAGCGTACAACGCGCAGGGGCTGGCGAACCg                             <  1:2263301/150‑1 (MQ=255)

                                                                                                                     gAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGc                            >  1:852905/1‑150 (MQ=255)

                                                                                                                     gAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGc                            >  1:126473/1‑150 (MQ=255)

                                                                                                                     gAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGc                            <  2:3497959/150‑1 (MQ=255)

                                                                                                                     gAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGc                            >  2:1177164/1‑150 (MQ=255)

                                                                                                                      aaGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGGAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGTGCAGGGGCTGGCGAACCGCt                           <  2:2715677/150‑1 (MQ=255)

                                                                                                                      aaGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCt                           <  1:3304423/150‑1 (MQ=255)

                                                                                                                       aGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTg                          >  2:2211428/1‑150 (MQ=255)

                                                                                                                        gggCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGt                         >  2:376156/1‑150 (MQ=255)

                                                                                                                            aCCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATAc                     >  2:471388/1‑150 (MQ=255)

                                                                                                                              cGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCg                   <  1:3438822/150‑1 (MQ=255)

                                                                                                                              cGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCg                   >  2:4033565/1‑150 (MQ=255)

                                                                                                                                   gACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAg              >  1:3944199/1‑150 (MQ=255)

                                                                                                                                       gTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTg          >  1:2768266/1‑150 (MQ=255)

                                                                                                                                        tGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGc         >  2:817365/1‑150 (MQ=255)

                                                                                                                                          cACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGGCATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGccc       >  1:1000837/1‑150 (MQ=255)

                                                                                                                                          cACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGccc       >  1:1962138/1‑150 (MQ=255)

                                                                                                                                              aTGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGGGTCCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCccgccg   >  1:90120/1‑150 (MQ=255)

‑‑‑‑‑‑‑‑‑CACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGT  >  NODE_92/1‑284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`