BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NODE_20	46,127	Δ1 bp	intergenic (+3/‑2)	EcoliAnna_02844 → / → EcoliAnna_02845	tRNA‑Val/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_20	46,126	0	T	.	100.0%	559.8 / NA	132	intergenic (+2/‑3)	EcoliAnna_02844/EcoliAnna_02845	tRNA‑Val/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (61/71); total (61/71)

Only 100 of 143 total aligned reads displayed.

GTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCG  >  NODE_20/45977‑46271

gTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACt                                                                                                                                                   >  2:1513585/1‑150 (MQ=255)

gTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACt                                                                                                                                                   >  2:739674/1‑150 (MQ=255)

 tCATCCTGCACGACCCACCACTATTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACtc                                                                                                                                                  >  2:1064991/1‑149 (MQ=255)

  cATCCTGCACGCCCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCACAGCTGGGAGAGCACCCCGCTTACAAGGAGGGGGACGGCGGTTCGATCCCGTCATCACCCACCACtcg                                                                                                                                                 <  1:4025609/150‑3 (MQ=21)

  cATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACtcg                                                                                                                                                 <  1:3610447/150‑3 (MQ=255)

   aTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACtcgg                                                                                                                                                >  1:1596624/1‑147 (MQ=255)

    tCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACtcggg                                                                                                                                               >  1:689568/1‑146 (MQ=255)

       tGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCg                                                                                                                                           <  1:3203183/150‑1 (MQ=255)

       tGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCg                                                                                                                                           <  2:2652308/150‑1 (MQ=255)

       tGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCg                                                                                                                                           <  2:371738/150‑1 (MQ=255)

        gCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCAC‑TCGGGTCGt                                                                                                                                          >  2:487276/1‑150 (MQ=255)

         cACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGtt                                                                                                                                         <  2:3419795/150‑1 (MQ=255)

          aCGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTa                                                                                                                                        <  2:1046982/150‑1 (MQ=255)

                caccacTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAg                                                                                                                                  <  2:3059356/150‑1 (MQ=255)

                   cacTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTg                                                                                                                               <  2:252382/150‑1 (MQ=255)

                       aaTGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTag                                                                                                                           <  1:878050/150‑1 (MQ=255)

                         tGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTagag                                                                                                                         <  1:2785852/150‑1 (MQ=255)

                           aCGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCa                                                                                                                       <  1:262170/150‑1 (MQ=255)

                           aCGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGAGTGATTAGCTCTGCTGGGTGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTAAGCTCAGTTGGGAGAGCa                                                                                                                       >  2:3097302/1‑150 (MQ=17)

                            cGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                                                                                                      <  2:2496230/150‑1 (MQ=255)

                            cGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                                                                                                      >  1:1009260/1‑150 (MQ=255)

                             ggTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                     <  2:2787905/150‑1 (MQ=255)

                             ggTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                     <  1:3226291/150‑1 (MQ=255)

                              gTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                                                                                    >  2:2470137/1‑150 (MQ=255)

                                 ggTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc                                                                                                                 >  2:552904/1‑150 (MQ=255)

                                  gTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                                                                                                                <  2:2005048/150‑1 (MQ=255)

                                    tCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGATCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                              >  2:188466/1‑150 (MQ=255)

                                    acGGAGGTAGTAGTTTGTAGTATCCAGCGCAGTAACGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                              <  2:76361/149‑1 (MQ=11)

                                      ggTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTa                                                                                                            >  1:3323369/1‑150 (MQ=255)

                                       gTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                                                                                           <  2:2117379/150‑1 (MQ=255)

                                        tGGAAGTAGTTTGTAGTATCCAGCGCAGTATGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                          <  1:2681632/150‑1 (MQ=255)

                                          gAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGATCAGTTGGGAGCGCAGTTGTCTTTTAATCa                                                                                                        >  1:2055496/1‑150 (MQ=11)

                                            agtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                      <  1:2065093/150‑1 (MQ=37)

                                               agtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                                                                                   <  2:1103472/150‑1 (MQ=21)

                                               agtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                                                                                   <  1:2567679/150‑1 (MQ=21)

                                                gtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                                                                                  <  2:501163/150‑1 (MQ=18)

                                                    gtagtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCa                                                                                              >  2:3668852/1‑150 (MQ=17)

                                                      agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg                                                                                            >  2:3369583/1‑150 (MQ=17)

                                                      agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg                                                                                            >  2:1751833/1‑150 (MQ=17)

                                                       gtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAGTTTTAATCAATTGGTCGCAgct                                                                                           >  2:1806053/1‑148 (MQ=12)

                                                       gtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGCCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGAAGAGCAGTTGACTTTTAATCAACTGGTCGCAGGt                                                                                           >  1:1037470/1‑150 (MQ=255)

                                                            cAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaa                                                                                      >  2:1724328/1‑150 (MQ=17)

                                                             aGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                     >  2:1962876/1‑150 (MQ=17)

                                                             aGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                     <  1:3426152/150‑1 (MQ=17)

                                                             aGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                                                     <  2:689568/150‑1 (MQ=17)

                                                             aGCCCAGTAACGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTCACAAGGAGGGGGTCGGCGGCTCGGTCCCGTCATCACCCACCAC‑TCGCGTCGTTAGCTTAGTTCGAAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaag                                                                                     >  2:1014459/1‑149 (MQ=11)

                                                               cgcAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                                                                                   >  2:2576243/1‑150 (MQ=17)

                                                                gcAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                                  <  2:3487542/150‑1 (MQ=14)

                                                                 cAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTAGTCGCAGGTTCGAATCCTg                                                                                 <  1:1355784/150‑1 (MQ=2)

                                                                                          ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCaa                                                        <  2:1452474/150‑1 (MQ=14)

                                                                                          ggAGAGCACCTCCCTTACAAGGAGGGGGACGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCaa                                                        <  2:176712/150‑1 (MQ=9)

                                                                                            agagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAAc                                                      <  2:3501994/150‑1 (MQ=17)

                                                                                             gagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACt                                                     <  2:1384296/150‑1 (MQ=18)

                                                                                               gCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCa                                                   >  1:3350135/1‑150 (MQ=25)

                                                                                               gCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCa                                                   <  2:4045760/150‑1 (MQ=25)

                                                                                                 aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGTTCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGACCAGTTGAGTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAGTTTTAACATCAAACTCAGa                                                 >  1:1613876/1‑150 (MQ=11)

                                                                                                 aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                 >  2:745859/1‑150 (MQ=37)

                                                                                                  ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                <  1:2835341/150‑1 (MQ=37)

                                                                                                  ccTCCCTTACAAGGAGGGGGACGGCGGTCCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                <  1:2744018/150‑1 (MQ=255)

                                                                                                   cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGCTg                                               >  1:1737310/1‑150 (MQ=255)

                                                                                                   cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATg                                               >  2:3938090/1‑150 (MQ=37)

                                                                                                    tCCCTTACAAGGAGGGGGTCGGCGGTTCGATGCCGGCATCACCCACCAC‑CCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAATCAATTGGTCGCAGGTTCGAATGCTGCACGACGCACCAGTTTTAACATCAAACTCAGATGt                                              <  2:659033/150‑1 (MQ=11)

                                                                                                    tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGt                                              >  2:3344191/1‑150 (MQ=37)

                                                                                                      ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTa                                            >  2:1815711/1‑150 (MQ=37)

                                                                                                       cTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTaa                                           >  2:1594459/1‑150 (MQ=37)

                                                                                                        ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTGGTTAGCTCAGTTGGTATAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGGATCCTGCACGAGCCACCAGTTTTTACATCAAACTCAGTTGTTatg                                          >  1:3597610/1‑148 (MQ=11)

                                                                                                          aCAAGGAGGGGGTCGGCGGATCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCg                                        <  2:1634301/150‑1 (MQ=255)

                                                                                                           cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGt                                       >  2:2326731/1‑150 (MQ=37)

                                                                                                             aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                     >  1:3892308/1‑150 (MQ=255)

                                                                                                                 gggtgTCGGCGGTTCGTTCCCGTCATCACCCGCCAC‑TGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACGTTTAATCAATTGGTCGCAGGTTCGAATCCTACTCGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGa                                 <  2:3432649/150‑1 (MQ=14)

                                                                                                                  ggggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGAt                                <  2:145334/150‑1 (MQ=255)

                                                                                                                  ggggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGAt                                <  1:13550/150‑1 (MQ=255)

                                                                                                                  ggggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGAAt                                <  1:52743/150‑1 (MQ=255)

                                                                                                                   gggTGGGCGGTGCGATCCCGTCATCACCCACCGC‑TCGGGTCGTTAGCGCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGACGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAGCTCAGATGTTAAGCGTGAAGGATa                               <  1:3855848/150‑1 (MQ=14)

                                                                                                                     gTCGGCGGTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTAAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGCATCCTGCACGACCCACCAGTATTAACATCAAACTCAGATGTTAAGCGTGAAGGATAAc                             >  1:605044/1‑150 (MQ=255)

                                                                                                                       cggcggTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGt                           >  1:1277528/1‑150 (MQ=255)

                                                                                                                        ggcggTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                          >  1:1246800/1‑150 (MQ=255)

                                                                                                                        ggcggTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGCTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                          <  2:2896026/150‑1 (MQ=255)

                                                                                                                         gcggTTCGATCCCGCCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTg                         <  2:2647692/150‑1 (MQ=255)

                                                                                                                          cggTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgc                        <  1:15038/150‑1 (MQ=255)

                                                                                                                            gTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcgc                      <  2:612000/150‑1 (MQ=255)

                                                                                                                            gTTCGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcgc                      >  1:1827461/1‑150 (MQ=255)

                                                                                                                               cGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTTAGATGTTAAGCGTGAAGGATAACGTTGCGCCAg                   >  1:1018607/1‑150 (MQ=255)

                                                                                                                               cGATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAg                   <  2:3176697/150‑1 (MQ=255)

                                                                                                                                gATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGc                  <  1:2161432/150‑1 (MQ=255)

                                                                                                                                gATCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGc                  >  1:1450737/1‑150 (MQ=255)

                                                                                                                                 aTCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCa                 >  2:1839104/1‑150 (MQ=255)

                                                                                                                                  tCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGCCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCaa                <  1:2264080/150‑1 (MQ=255)

                                                                                                                                  tCCCGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCaa                <  1:683050/150‑1 (MQ=255)

                                                                                                                                   cccGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAAc               <  2:2199305/150‑1 (MQ=255)

                                                                                                                                    ccGTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACg              <  2:1246800/150‑1 (MQ=255)

                                                                                                                                      gTCATCACCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAGATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc            >  1:276863/1‑150 (MQ=255)

                                                                                                                                        catcaCCCACCAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGccc          <  1:3722047/150‑1 (MQ=255)

                                                                                                                                           cacccaccAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTa       >  2:2450022/1‑150 (MQ=255)

                                                                                                                                           cacccaccAC‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTa       >  2:35553/1‑150 (MQ=255)

                                                                                                                                               caccac‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgc   >  1:1445803/1‑150 (MQ=255)

                                                                                                                                                accac‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg  >  1:933971/1‑150 (MQ=255)

                                                                                                                                                accac‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg  >  2:154155/1‑150 (MQ=255)

                                                                                                                                                accac‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg  >  1:4020386/1‑150 (MQ=255)

                                                                                                                                                accac‑TCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg  <  1:2038603/150‑1 (MQ=255)

GTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCG  >  NODE_20/45977‑46271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`