BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NODE_20	45,821	Δ2 bp	intergenic (+2/‑2)	EcoliAnna_02842 → / → EcoliAnna_02843	tRNA‑Val/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_20	45,821	0	T	.	100.0%	627.0 / NA	150	intergenic (+2/‑3)	EcoliAnna_02842/EcoliAnna_02843	tRNA‑Val/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (67/83); total (67/83)
*	NODE_20	45,822	0	T	.	100.0%	627.0 / NA	150	intergenic (+3/‑2)	EcoliAnna_02842/EcoliAnna_02843	tRNA‑Val/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (67/83); total (67/83)

Only 100 of 150 total aligned reads displayed.

CGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGG  >  NODE_20/45679‑45963

||

cGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGt                                                                                                                                       <  1:2793114/150‑1 (MQ=255)

cGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGt                                                                                                                                       >  2:1322304/1‑150 (MQ=255)

  aaGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTa                                                                                                                                     >  2:3664116/1‑150 (MQ=255)

   aGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAg                                                                                                                                    >  1:1206920/1‑150 (MQ=255)

   aGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAg                                                                                                                                    >  1:3120168/1‑150 (MQ=255)

    gTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGGTAGc                                                                                                                                   >  1:2229953/1‑150 (MQ=255)

     tCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCt                                                                                                                                  >  1:2067826/1‑150 (MQ=255)

       gAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCa                                                                                                                                >  2:4084101/1‑150 (MQ=255)

        aGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGACCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAg                                                                                                                               <  2:2321069/150‑1 (MQ=255)

         gTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGt                                                                                                                              <  1:2602085/150‑1 (MQ=255)

           cATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTg                                                                                                                            <  1:932184/150‑1 (MQ=255)

           cATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTg                                                                                                                            >  2:3423730/1‑150 (MQ=255)

            aTCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTgg                                                                                                                           <  2:2554400/150‑1 (MQ=255)

            aTCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTgg                                                                                                                           >  2:4126178/1‑150 (MQ=255)

            aTCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTgg                                                                                                                           <  1:1287284/150‑1 (MQ=255)

             tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGt                                                                                                                          >  2:3844132/1‑150 (MQ=255)

                 gCACGACCCAGCACTAACATAGTTAGTTGTAGTATTCAGCGTAGTAGCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTagag                                                                                                                      <  2:1151661/150‑1 (MQ=17)

                  cACGACCCACCACAAACATAGTTAGTAGTAGTATGCAGCGTAATATCGGGTGATTAGATCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGc                                                                                                                     <  1:2943923/150‑1 (MQ=17)

                   aCGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCa                                                                                                                    <  2:1799871/150‑1 (MQ=255)

                    cGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAg                                                                                                                   <  1:3846602/150‑1 (MQ=255)

                     gACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGGGCAGt                                                                                                                  >  1:2324234/1‑150 (MQ=255)

                     gACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                  >  1:3436380/1‑150 (MQ=255)

                     gACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGGTAGCTCAGTTGGTAGAGCAGt                                                                                                                  >  2:664313/1‑150 (MQ=255)

                      aCCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                                                                                 <  1:2623395/150‑1 (MQ=255)

                       cccACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                                                                                                <  1:2385385/150‑1 (MQ=255)

                         caccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc                                                                                                              <  2:738741/150‑1 (MQ=255)

                         caccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc                                                                                                              <  1:1938054/150‑1 (MQ=255)

                               tAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGGGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGCTAGAGCCGTTGACTTTTaa                                                                                                        >  1:1865272/1‑150 (MQ=14)

                                aaCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                       <  2:1018185/150‑1 (MQ=255)

                                aaCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                       >  1:1604490/1‑150 (MQ=255)

                                aaCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                       <  2:127056/150‑1 (MQ=255)

                                aaCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                       >  1:222046/1‑150 (MQ=255)

                                 aCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                      >  1:771772/1‑150 (MQ=255)

                                 aCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                      <  2:771772/150‑1 (MQ=255)

                                 aCATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCACTTACAAGGAGGGGGTCGGCGGTTCGATCCCGCCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                                                                                                      <  1:2870325/150‑1 (MQ=255)

                                   aTAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                    <  1:2300715/150‑1 (MQ=255)

                                   aTAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCACAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa                                                                                                    <  1:178385/150‑1 (MQ=255)

                                     agttagttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                  <  1:1046171/150‑1 (MQ=255)

                                     agttagttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                                                                                                  >  2:2431557/1‑150 (MQ=255)

                                         agttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc                                                                                              <  1:805750/150‑1 (MQ=16)

                                          gttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg                                                                                             <  1:3382051/150‑1 (MQ=12)

                                           ttGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGc                                                                                            >  2:1914605/1‑150 (MQ=12)

                                            tGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCa                                                                                           >  1:3906309/1‑150 (MQ=12)

                                             gtagtaTCCAGCGTAGTATCGGGTGATTAGCCCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                                                                                          <  2:2625781/150‑1 (MQ=12)

                                              tagtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg                                                                                         <  2:1458432/150‑1 (MQ=12)

                                              tagtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg                                                                                         <  2:4052913/150‑1 (MQ=12)

                                                gtaTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                       <  2:2534273/150‑1 (MQ=12)

                                                gtaTCCAGCGTAGTATCGGGTGATTAGCCCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                       <  2:1723752/150‑1 (MQ=12)

                                                 taTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                      <  1:1852255/150‑1 (MQ=12)

                                                  aTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGTGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTTATCAATTGGTCGCAGGTTCg                                                                                     >  2:3740299/1‑150 (MQ=255)

                                                  aTCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                                                                                     >  1:3890304/1‑150 (MQ=12)

                                                    ccAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGaa                                                                                   >  2:4122535/1‑150 (MQ=12)

                                                    ccAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCCaa                                                                                   >  1:1898067/1‑150 (MQ=12)

                                                      aGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGGTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATc                                                                                 >  2:2378249/1‑150 (MQ=12)

                                                       gCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                                                                                >  1:3804832/1‑150 (MQ=12)

                                                       gCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                                                                                <  1:4073293/150‑1 (MQ=12)

                                                        cGTAGTGTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGACATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt                                                                               <  1:3175991/150‑1 (MQ=11)

                                                         gtagtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTg                                                                              <  1:4122535/150‑1 (MQ=9)

                                                          tagtaTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                                                                             <  2:1313710/150‑1 (MQ=2)

                                                                                 tGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCg                                                      >  2:2659770/1‑150 (MQ=2)

                                                                                   ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGaa                                                    <  2:525509/150‑1 (MQ=12)

                                                                                   ggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGaa                                                    >  2:3447766/1‑150 (MQ=12)

                                                                                    gagaGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAg                                                   <  2:970005/150‑1 (MQ=12)

                                                                                      gagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGAc                                                 <  2:1216765/150‑1 (MQ=19)

                                                                                       agCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACa                                                >  2:2096133/1‑150 (MQ=255)

                                                                                       agCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACa                                                >  2:497557/1‑150 (MQ=255)

                                                                                         cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGa                                              >  1:1551453/1‑150 (MQ=255)

                                                                                          aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGAt                                             <  1:987518/150‑1 (MQ=255)

                                                                                          aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGAt                                             <  2:2738765/150‑1 (MQ=255)

                                                                                           ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATg                                            <  2:3042271/150‑1 (MQ=255)

                                                                                             tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAACTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGtt                                          <  2:940050/150‑1 (MQ=255)

                                                                                               ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCACAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTaa                                        <  1:488229/150‑1 (MQ=255)

                                                                                               ccTTACAAGGAGGGGGTCGGCGGGTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGGGCAGTTGTCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGGTaa                                        >  2:1174481/1‑150 (MQ=11)

                                                                                                 ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCTCCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGc                                      <  1:4126178/150‑1 (MQ=255)

                                                                                                 ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGc                                      <  2:3826835/150‑1 (MQ=255)

                                                                                                  tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCCCGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCg                                     <  1:2378249/150‑1 (MQ=255)

                                                                                                    cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCgtg                                   >  2:3380816/1‑150 (MQ=255)

                                                                                                      aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTa                                 <  2:1764589/150‑1 (MQ=255)

                                                                                                          gggggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGAt                             <  1:1388236/150‑1 (MQ=255)

                                                                                                            gggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATaa                           >  2:1410429/1‑150 (MQ=255)

                                                                                                             ggTCGGCGGTTCGATCCGGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTGGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAAc                          <  1:904308/150‑1 (MQ=255)

                                                                                                             ggTCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAAc                          >  1:1724455/1‑150 (MQ=255)

                                                                                                               tCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGt                        <  1:4074856/150‑1 (MQ=255)

                                                                                                               tCGGCGGTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGt                        <  2:3263284/150‑1 (MQ=255)

                                                                                                                 ggcggTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTg                      >  2:3000931/1‑150 (MQ=255)

                                                                                                                  gccgTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGc                     <  1:674290/147‑1 (MQ=255)

                                                                                                                    ggTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGGGTGTAGGATAACGTTGCGt                   >  1:802880/1‑150 (MQ=255)

                                                                                                                    ggTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGt                   >  2:885942/1‑150 (MQ=255)

                                                                                                                     gTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGGACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGGTAACGTTGGGTc                  >  2:3776344/1‑150 (MQ=14)

                                                                                                                     gTTCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTc                  <  1:2096133/150‑1 (MQ=255)

                                                                                                                      ttCGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCa                 <  1:1389702/150‑1 (MQ=255)

                                                                                                                        cGATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGc               <  1:3390319/150‑1 (MQ=255)

                                                                                                                         gATCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCa              >  2:2698735/1‑150 (MQ=255)

                                                                                                                          aTCCCGTCATCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCaa             >  2:3320628/1‑150 (MQ=255)

                                                                                                                           ccccGTCACCACCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAAc            <  1:466871/149‑1 (MQ=14)

                                                                                                                                tcatcaCCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTGGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGccc       <  1:2980908/150‑1 (MQ=255)

                                                                                                                                tcatcaCCCACCAC‑‑CGGGCCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGCCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGccc       <  1:1011560/150‑1 (MQ=255)

                                                                                                                                  atcaCCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGt     >  2:154667/1‑150 (MQ=255)

                                                                                                                                  atcaCCCACCAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGCCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGt     <  1:3844132/150‑1 (MQ=255)

                                                                                                                                    cacccaccAC‑‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAg   >  2:2238233/1‑150 (MQ=255)

||

CGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGG  >  NODE_20/45679‑45963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`