BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NODE_10	108,811	Δ1 bp	coding (87/1257 nt)	EcoliAnna_01894 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NODE_10	108,811	0	G	.	98.8%	1312.8 / 6.4	255	coding (87/1257 nt)	EcoliAnna_01894	MFS transporter
Reads supporting (aligned to +/- strand): ref base G (2/1); new base . (126/126); total (128/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 263 total aligned reads displayed.

CTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACC  >  NODE_10/108664‑108955

ctcGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAtttt                                                                                                                                                >  2:796951/1‑150 (MQ=255)

  cGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATat‑tttgt                                                                                                                                             >  2:3442715/1‑150 (MQ=255)

  cGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATat‑tttgt                                                                                                                                             >  2:3100008/1‑150 (MQ=255)

   gCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATat‑tttgtt                                                                                                                                            <  2:329769/150‑1 (MQ=255)

      cGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTAcc                                                                                                                                         >  2:4008244/1‑150 (MQ=255)

       ggTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGTCGACCCTCGGCTCCTATAT‑TTTGGTAccc                                                                                                                                        >  1:1732702/1‑150 (MQ=255)

       ggTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTAccc                                                                                                                                        >  2:1905173/1‑150 (MQ=255)

       ggTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTAccc                                                                                                                                        >  2:2383084/1‑150 (MQ=255)

          cAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTCCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGa                                                                                                                                     >  2:1086124/1‑150 (MQ=255)

           aaaTGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGaa                                                                                                                                    >  1:2551634/1‑150 (MQ=255)

           aaaTGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGaa                                                                                                                                    >  2:3040406/1‑150 (MQ=255)

            aaTGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAg                                                                                                                                   >  2:3093669/1‑150 (MQ=255)

            aaTGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAg                                                                                                                                   >  1:3235415/1‑150 (MQ=255)

             aTGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGt                                                                                                                                  <  2:2155206/150‑1 (MQ=255)

                tgCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAATCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTg                                                                                                                               >  2:764510/1‑150 (MQ=255)

                  ttttttCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGGTGGCTGGCGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGAc                                                                                                                             >  2:133672/2‑150 (MQ=255)

                  cTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGTAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGTAGTAGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAACTTTGAc                                                                                                                             >  1:1578868/1‑150 (MQ=255)

                  cTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGAc                                                                                                                             >  2:1866345/1‑150 (MQ=255)

                     tttCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGt                                                                                                                          >  2:3712804/1‑150 (MQ=255)

                      ttCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTg                                                                                                                         >  2:1427486/1‑150 (MQ=255)

                       tCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGc                                                                                                                        <  2:2419642/150‑1 (MQ=255)

                        cAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCt                                                                                                                       >  1:2891062/1‑150 (MQ=255)

                        cAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCt                                                                                                                       >  2:126133/1‑150 (MQ=255)

                         aaaCACTCATCCGCATCACGGTGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAGTCCTCTCTTTTCTGCAGTTCTGTCGGTGGCGAAGTCGTCTGGCGGCCCTCGGCTCCTATAT‑TTTGTTACCCTGATGTTTGTCGGTGttt                                                                                                                      >  1:67512/1‑150 (MQ=255)

                         aaaCACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCtt                                                                                                                      >  1:1328710/1‑150 (MQ=255)

                           acacTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGGCGGCCCTCGGCTCCTTTAT‑TTTGTTACCCTGTAGTTTGACGGTGGTTCt                                                                                                                    >  1:2367032/1‑150 (MQ=255)

                           acacTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCt                                                                                                                    >  2:3822151/1‑150 (MQ=255)

                            cacTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTa                                                                                                                   >  2:1784523/1‑150 (MQ=255)

                               tCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTg                                                                                                                <  2:2259423/150‑1 (MQ=255)

                                cATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTgg                                                                                                               >  2:3908317/1‑150 (MQ=255)

                                 aTCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGgc                                                                                                              >  2:1482598/1‑150 (MQ=255)

                                   ccGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGgcg                                                                                                             >  2:2688780/1‑150 (MQ=255)

                                   ccGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGgcgc                                                                                                            <  1:3841582/150‑1 (MQ=255)

                                     gCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAg                                                                                                          >  1:1639648/1‑150 (MQ=255)

                                      cATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTCCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGt                                                                                                         <  1:2322112/150‑1 (MQ=255)

                                       aTCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGtt                                                                                                        >  2:678416/1‑150 (MQ=255)

                                       aTCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGtt                                                                                                        >  1:1525086/1‑150 (MQ=255)

                                       aTCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGGCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGtt                                                                                                        >  2:2614248/1‑150 (MQ=255)

                                        tCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGgtt                                                                                                       >  2:1093931/1‑150 (MQ=255)

                                          aCGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTtat                                                                                                     <  2:2907752/150‑1 (MQ=255)

                                            gATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAg                                                                                                   >  2:4081396/1‑150 (MQ=255)

                                            gATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAg                                                                                                   <  1:1019384/150‑1 (MQ=255)

                                            gATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAg                                                                                                   <  1:2866514/150‑1 (MQ=255)

                                            gATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAg                                                                                                   >  2:345936/1‑150 (MQ=255)

                                             aTGTGAGGAAATTAACATGAATCTTAAGCTTCAGCTGAAAATCCTTTTTTTTCTGCAGTTCTGTGTGTGGGGAAGTTGGCTGACGACCCTCGGCTTCTATAT‑TTTTTTACACTGAAGTTTGACTGTGCTTCTATTGGCGCAGATTATAGc                                                                                                  >  1:1466291/1‑150 (MQ=255)

                                               gtgAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGGAGTTTGACGGTGGTTCTATTGGCGCAGTTTATAGCTc                                                                                                >  1:2682775/1‑150 (MQ=255)

                                                tgAGGAAATTAACATGAATCTTAAGCTGCAGCAGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCa                                                                                               <  2:87890/150‑1 (MQ=255)

                                                  aGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACt                                                                                             >  1:2304171/1‑150 (MQ=255)

                                                    gAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTgg                                                                                           >  1:3395817/1‑150 (MQ=255)

                                                     aaaTTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATTGCTCACTggg                                                                                          >  2:986987/1‑150 (MQ=255)

                                                        ttAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTAt                                                                                       <  1:1623990/150‑1 (MQ=255)

                                                         tAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGTCGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATc                                                                                      >  1:3127923/1‑150 (MQ=255)

                                                          aaCATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCg                                                                                     <  2:1601439/150‑1 (MQ=255)

                                                           aCATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTACAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGc                                                                                    <  1:3476713/150‑1 (MQ=255)

                                                             aTGAATCTTAAGCTGCAGCTGAAAATCCACTCTTTTCTGCAGTTCTGTCTGTGGTTAAGTTGGCTGTTGACCCTCGTCTCCTATAT‑TTTGTTATCCTGGAGTTTGATGGTGCTTCTATAGGGGCTGATTTTAGCTCACTGAGTATCGCAg                                                                                  >  2:17369/1‑150 (MQ=255)

                                                              tGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGc                                                                                 <  2:2278167/150‑1 (MQ=255)

                                                              ggAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGc                                                                                 <  2:246831/149‑1 (MQ=255)

                                                                aaTCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCgg                                                                               >  2:4135469/1‑150 (MQ=255)

                                                                aaTCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCgg                                                                               <  1:993953/150‑1 (MQ=255)

                                                                aaTCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCgg                                                                               >  1:3496429/1‑150 (MQ=255)

                                                                 aTCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTCGGTATCGCAGCggg                                                                              >  1:2041209/1‑149 (MQ=255)

                                                                  tCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTc                                                                             <  2:2118840/150‑1 (MQ=255)

                                                                     tAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCttt                                                                          <  1:891600/150‑1 (MQ=255)

                                                                                tGAAAATCGTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCgct                                                               <  1:1051568/150‑1 (MQ=255)

                                                                                 gAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCgctg                                                              >  2:287697/1‑150 (MQ=255)

                                                                                         tctctTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGa                                                       <  1:485532/150‑1 (MQ=255)

                                                                                         tctctTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGAt                                                      <  1:1678645/150‑1 (MQ=255)

                                                                                          ctctTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGAtt                                                     <  1:3099282/150‑1 (MQ=255)

                                                                                            ctTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATtgt                                                   <  2:3550420/150‑1 (MQ=255)

                                                                                             ttttCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATtgtg                                                  <  1:2737466/150‑1 (MQ=255)

                                                                                             ttttCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATtgtg                                                  >  2:1820603/1‑150 (MQ=255)

                                                                                              tttCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTgg                                                 >  2:2343736/1‑150 (MQ=255)

                                                                                               ttCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGc                                                <  1:2458463/150‑1 (MQ=255)

                                                                                                tCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGcc                                               <  1:3606720/150‑1 (MQ=255)

                                                                                                tCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGcc                                               <  1:2189965/150‑1 (MQ=255)

                                                                                                tCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTAACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGcc                                               <  1:732660/150‑1 (MQ=255)

                                                                                                 cTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCg                                              >  2:3696302/1‑150 (MQ=255)

                                                                                                  tGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGTCGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGa                                             >  2:3229531/1‑150 (MQ=255)

                                                                                                   gCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGAc                                            <  2:645597/150‑1 (MQ=255)

                                                                                                     aGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACaa                                          <  2:3593964/150‑1 (MQ=255)

                                                                                                      gTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACaaa                                         <  2:1558863/150‑1 (MQ=255)

                                                                                                       ttCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAAt                                        <  1:3888312/150‑1 (MQ=255)

                                                                                                         ctgtctgtGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATgg                                      <  2:468515/150‑1 (MQ=255)

                                                                                                          tgtctgtGGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGt                                     <  2:4086095/150‑1 (MQ=255)

                                                                                                               gtgGGGAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGt                                <  1:4074551/150‑1 (MQ=255)

                                                                                                                 ggggAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGc                              <  1:599615/150‑1 (MQ=255)

                                                                                                                 ggggAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGc                              >  2:2861170/1‑150 (MQ=255)

                                                                                                                   ggAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGa                            <  2:163367/150‑1 (MQ=255)

                                                                                                                   ggAAGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGa                            <  1:3798071/150‑1 (MQ=255)

                                                                                                                      aGTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAAt                         <  2:2185703/150‑1 (MQ=255)

                                                                                                                       gTTGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATg                        <  1:1866345/150‑1 (MQ=255)

                                                                                                                        ttGGCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATgg                       <  1:20736/150‑1 (MQ=255)

                                                                                                                           gCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGta                    <  1:3039446/150‑1 (MQ=255)

                                                                                                                           gCTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGta                    >  2:2792617/1‑150 (MQ=255)

                                                                                                                            cTGACGACCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGtat                   <  2:3194927/150‑1 (MQ=255)

                                                                                                                                cgacCCTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGc               <  2:2535217/150‑1 (MQ=255)

                                                                                                                                    ccTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGAGGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCAtt           <  1:1189550/150‑1 (MQ=255)

                                                                                                                                     cTCGGCTCCTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCAttt          <  1:3280143/150‑1 (MQ=255)

                                                                                                                                            ccTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCcaca    >  2:1035003/1‑150 (MQ=255)

                                                                                                                                             cTATAT‑TTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACAcc  <  1:1482598/150‑1 (MQ=255)

CTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACC  >  NODE_10/108664‑108955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`