| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 61,050 | 4 bp→CCCT | coding (156‑159/360 nt) | SAUSA300_RS00265 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 61,050 | 0 | A | C | 98.7% | 209.8 / ‑5.8 | 77 | I53M (ATT→ATG) | SAUSA300_RS00265 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (7/69); minor base T (0/1); total (7/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| * | NC_007793 | 61,051 | 0 | A | C | 100.0% | 220.0 / NA | 77 | I53S (ATT→AGT) | SAUSA300_RS00265 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (7/70); total (7/70) | |||||||||||
| * | NC_007793 | 61,052 | 0 | T | C | 100.0% | 222.6 / NA | 77 | I53V (ATT→GTT) | SAUSA300_RS00265 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/70); total (7/70) | |||||||||||
| * | NC_007793 | 61,053 | 0 | G | T | 100.0% | 215.9 / NA | 77 | F52L (TTC→TTA) | SAUSA300_RS00265 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (7/70); total (7/70) | |||||||||||
ATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTT > NC_007793/61000‑61140 |||| tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc < 1:1342785/90‑1 (MQ=255)tgctcttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTCTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATcc < 1:1447598/90‑1 (MQ=255) tcttccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa < 1:2455282/90‑1 (MQ=255) gctcatccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCa < 1:220221/91‑1 (MQ=255) ttccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAt < 1:786688/91‑1 (MQ=255) tcttccgaTCTGCCTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATa < 1:1142155/93‑1 (MQ=255) tccgatcttCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCAtg < 1:4467611/92‑2 (MQ=255) cttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATAc < 1:3360591/94‑1 (MQ=255) ttccggtCTGCCTATACAAAAACTAATCCATGTCAGAATATGATTTCCTAAAGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa < 1:4409056/94‑1 (MQ=255) ttccgaTCTGCCTAGACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa < 1:4724217/95‑1 (MQ=255) ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa < 1:5716041/95‑1 (MQ=255) ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa < 1:5224148/95‑1 (MQ=255) ttccgaTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa < 1:2485526/95‑1 (MQ=255) ccgatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACa < 1:2334305/94‑1 (MQ=255) gatctCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACaaa < 1:2776804/96‑1 (MQ=255) aTCTGCCTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATaa < 1:3021366/100‑1 (MQ=255) gcctgcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCCTTTTTACCACGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt > 1:312784/9‑100 (MQ=255) gccTATACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt < 1:49580/99‑1 (MQ=255) gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt > 1:4033749/2‑100 (MQ=255) gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt > 1:3785813/2‑100 (MQ=255) gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt > 1:3628319/2‑100 (MQ=255) gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt < 1:4712518/99‑1 (MQ=255) gccTACACAAAAACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGt > 1:5478884/2‑100 (MQ=255) gccTACACAAAAACTAATCCATGTCATAATATGAGTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGGTCATCAGCAAGTATCCATACAAATAAAGGt < 1:3935916/99‑1 (MQ=255) cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa < 1:2432831/93‑1 (MQ=255) cctccacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTa < 1:5467683/93‑1 (MQ=255) cggcacaaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGCCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTaa < 1:3248254/94‑1 (MQ=255) ataCAAAAACTAATCCATGTCATAATATGATTCCCTAAGGCGTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTagca < 1:2640796/100‑4 (MQ=255) aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc < 1:5555394/100‑1 (MQ=255) aaaaACTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGAc < 1:4624969/100‑1 (MQ=255) aaaCTAATCCATGTCATAGTATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCTAGTATCCATACAAATAAAGGTAAAATGACAg < 1:5038059/98‑1 (MQ=255) aCTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGt < 1:249309/100‑1 (MQ=255) aCTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGt < 1:2541477/100‑1 (MQ=255) cTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGta < 1:5611630/100‑2 (MQ=255) cTAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTg < 1:4085334/100‑1 (MQ=255) tAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGc < 1:2022312/100‑1 (MQ=255) tAATCCATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGc < 1:377691/100‑1 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATGCAAATAAAGGTAAAATGACAGGTGCg < 1:2132753/97‑1 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGATAGGTGCg > 1:2692673/4‑100 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGcgcg > 1:1708366/4‑100 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGct < 1:420304/97‑2 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg < 1:5430265/97‑1 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg < 1:3301640/97‑1 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg < 1:4201379/97‑1 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg < 1:4207953/97‑1 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg < 1:5503437/97‑1 (MQ=255) aatccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCg < 1:4386860/97‑1 (MQ=255) aatccATGTCATAATATGAGTCCCTAATGCTTTGTTACCATGTCCGGATGTTAGCTCTTCAGCAAGTATCCATACAAATAAAGTTAAAATGACAGGTGCg < 1:3495627/97‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCgt < 1:2342875/98‑2 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:4870723/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:4822449/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:2345561/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:1385751/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:5034526/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:5436286/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:2341241/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGa < 1:2855824/98‑1 (MQ=255) atccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCAGCAGCAAGTATCCATACAAATAAAGGTAAGATGACAGGTGCGa < 1:2585414/98‑1 (MQ=255) tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa < 1:620046/99‑1 (MQ=255) tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa < 1:916508/99‑1 (MQ=255) tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa < 1:4538667/99‑1 (MQ=255) tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa < 1:3704873/99‑1 (MQ=255) tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa < 1:3085020/99‑1 (MQ=255) tccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaa < 1:2565557/99‑1 (MQ=255) ccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaa < 1:5503416/100‑1 (MQ=255) ccATGTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaa < 1:5650104/100‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:4337639/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:1214596/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:740667/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:1860485/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:1993825/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:2652408/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:3409549/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:3450811/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:3515593/97‑1 (MQ=255) catgTCATAATATGATTCCCTAATGCTTTTTTACCATGTCCTGATGTTGGCTCACCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGaaaa < 1:100971/97‑1 (MQ=255) taataTGATTCCCTAATGC‑TTTTTACCATGTCCTGATGTTGGCTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACtt < 1:15794/100‑1 (MQ=255) |||| ATAAATGCCAATCTACCTATAAAAAGACTAACCCAAGTCATAATATGATTAATGAATGCTTTTTTGCCATGTTCTGATGTTGGTTCATCAGCAAGTATCCATACAAATAAAGGTAAAATGACAGGTGCGAAAAAGATACTT > NC_007793/61000‑61140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |