Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	60,940	2 bp→GC	coding (268‑269/360 nt)	SAUSA300_RS00265 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	60,940	0	A	G	100.0%	10.4 / NA	6	L90P (CTT→CCT)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/5);  total (1/5)
*	NC_007793	60,941	0	G	C	100.0%	11.8 / NA	6	L90V (CTT→GTT)	SAUSA300_RS00265	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (1/5);  total (1/5)

TTAATTCCTCTGTTAATGTTCAAGATATATAATATTAAAGCAATTAAAAAGAATATAATAGTAATAATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCT  >  NC_007793/60868‑61013                                                                         ||                                                                         ttAATTCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTgatgat                                                <  1:2166268/100‑1 (MQ=255)    tctCCTCTGATAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATcaa                                             <  1:4345723/98‑3 (MQ=255)     ccgatctGATAACGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATcaag                                            <  1:4480747/96‑4 (MQ=255)          ctGATAATGTTGAGAATATATAATATTAAGGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATCAAACGGtt                                       <  1:1037166/100‑1 (MQ=255)            gatAATGTTGAGAATATATAATATTAAAGCAATTAAAAAGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATCAAACGGttag                                     >  1:5250923/3‑98 (MQ=255)                                               aaaaGAATACAATAGCAATGATTAAAGCAATAACAAATATTAATAATTGATGATCAAACGGTTTATCAAAGACTCCTTTTGAAAaaataaaaattccagc  <  1:4454308/100‑10 (MQ=255)                                                                         ||                                                                         TTAATTCCTCTGTTAATGTTCAAGATATATAATATTAAAGCAATTAAAAAGAATATAATAGTAATAATTAAAAGAATGCTAAATATTAATAATTGATGATCCAAAGGTTTATCAAAGACTTCTTTAGAAAAAATAAATGCCAATCT  >  NC_007793/60868‑61013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑