Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,114,095	5 bp→GGCCA	coding (306‑310/405 nt)	SAUSA300_RS10725 ←	Holliday junction DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,114,095	0	A	G	100.0%	15.8 / NA	8	Y104H (TAT→CAT)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/5);  total (3/5)
*	NC_007793	2,114,096	0	A	G	100.0%	16.3 / NA	8	N103N (AAT→AAC)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/5);  total (3/5)
*	NC_007793	2,114,097	0	T	C	100.0%	17.3 / NA	8	N103S (AAT→AGT)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/5);  total (3/5)
*	NC_007793	2,114,098	0	T	C	100.0%	15.8 / NA	8	N103D (AAT→GAT)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/5);  total (3/5)
*	NC_007793	2,114,099	0	G	A	100.0%	17.4 / NA	8	N102N (AAC→AAT)	SAUSA300_RS10725	Holliday junction DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

GCTTTGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCACAAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAA  >  NC_007793/2114047‑2114166                                                 |||||                                                                    gatctgggCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGctt                      <  1:5668041/93‑1 (MQ=255)    ttGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCggctcaga                   >  1:1749587/1‑94 (MQ=255)    ttGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCggccagta                   <  1:707091/100‑7 (MQ=255)           aGTTATTTCTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACtttt            >  1:1250598/1‑100 (MQ=255)                   cTGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTcc    <  1:2261277/100‑1 (MQ=255)                    tGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCa   <  1:2027841/100‑1 (MQ=255)                    tGTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCa   <  1:2618373/100‑1 (MQ=255)                     gTAATTTGGTTATCGTCTTTCCATACATGGCCATTACAAGCATCTAATACCGTTTTAATTAAGTTGTCGATATCCGGCTTAGTCACTTTTAATTGTCCaa  >  1:4603679/1‑100 (MQ=255)                                                 |||||                                                                    GCTTTGAGCTAGTTATTTCTGTAATTTGGTTATCGTCTTTCCACAAATAATTGTTACAAGCGTCTAGAACTGTCTTCATCAAATTATCGATATCTGGTTTAGTTACTTTTAATTGTCCAA  >  NC_007793/2114047‑2114166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑