Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 1,929,115 3 bp→CTT intergenic (‑201/‑218) SAUSA300_RS09560 ← / → SAUSA300_RS09565 transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077931,929,1150AC96.6% 1711.5 / 28.9 643intergenic (‑201/‑220)SAUSA300_RS09560/SAUSA300_RS09565transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/10);  new base C (603/18);  total (615/28)
Fisher's exact test for biased strand distribution p-value = 1.99e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0077931,929,1160GT96.4% 1878.8 / 34.3 643intergenic (‑202/‑219)SAUSA300_RS09560/SAUSA300_RS09565transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (13/10);  new base T (601/18);  total (615/28)
Fisher's exact test for biased strand distribution p-value = 3.49e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0077931,929,1170GT96.6% 1884.9 / 32.1 643intergenic (‑203/‑218)SAUSA300_RS09560/SAUSA300_RS09565transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (12/10);  new base T (603/18);  total (615/28)
Fisher's exact test for biased strand distribution p-value = 1.99e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 643 total aligned reads displayed.

AAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGT  >  NC_007793/1929021‑1929194
                                                                                               |||                                                                             
aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAAGGTgg                                                                            <  1:829680/100‑2 (MQ=255)
                         tGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAAGGTGATGCTTATTTTCGTTCACATCATAGc                                                   >  1:1285572/1‑100 (MQ=255)
                                       taatagAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTgg                                    <  1:3973980/97‑1 (MQ=255)
                                          tagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                  <  1:5539550/100‑1 (MQ=255)
                                             ggTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                              >  1:5166340/2‑100 (MQ=255)
                                              ttCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                             >  1:1737472/2‑99 (MQ=255)
                                                cGCCTATCTCTCAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                             >  1:1986531/1‑99 (MQ=255)
                                                cGCCTATCTCTCAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                             >  1:3266326/1‑99 (MQ=255)
                                                         tccAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:4528238/3‑99 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCggat                    >  1:5743634/1‑97 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:4905941/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:4948973/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:3319661/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:912593/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:4089695/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:3144259/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:4194108/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:5245508/1‑98 (MQ=255)
                                                         ctcAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:3010573/1‑98 (MQ=255)
                                                         ctcAGGCGTCAA‑TCA‑ACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTAGCTGTGCTGTTGCGtatta                  >  1:2095562/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCgcatta                  >  1:5399684/1‑95 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:4993119/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:4052595/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:439533/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:2632339/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:5674547/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:2305763/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:2429145/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:634909/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:217007/1‑99 (MQ=255)
                                                         ctcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:2022838/1‑99 (MQ=255)
                                                         accAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                    >  1:2374941/3‑98 (MQ=255)
                                                          tcAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                 >  1:2924666/1‑98 (MQ=255)
                                                           cAGTCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                  >  1:3873095/1‑99 (MQ=255)
                                                           cAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                >  1:1546303/1‑97 (MQ=255)
                                                           cAGCCGTCAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtacac                  <  1:2978767/100‑5 (MQ=255)
                                                               cGTCAACTTAGGCGCAGCGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact              >  1:1664267/1‑95 (MQ=255)
                                                                  cAACTTAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt           >  1:4781522/1‑100 (MQ=255)
                                                                    ttgcaggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACAGCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg         >  1:3722939/8‑100 (MQ=255)
                                                                    acttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg         >  1:3579528/3‑100 (MQ=255)
                                                                     tttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg        >  1:4625841/1‑100 (MQ=255)
                                                                     cttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg        >  1:3219216/2‑100 (MQ=255)
                                                                     cttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg        >  1:3345885/2‑100 (MQ=255)
                                                                     ctaaggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg        >  1:1485823/7‑100 (MQ=255)
                                                                     cgtAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg        >  1:2597264/3‑100 (MQ=255)
                                                                      ttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:1147149/1‑100 (MQ=255)
                                                                      ttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:2514080/1‑100 (MQ=255)
                                                                      ttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:2206382/1‑100 (MQ=255)
                                                                      ttAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:5492589/1‑100 (MQ=255)
                                                                      gcaggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:1312625/6‑100 (MQ=255)
                                                                      ctAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:1006017/2‑100 (MQ=255)
                                                                      ccaggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:2666242/6‑100 (MQ=255)
                                                                      atAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:5095349/2‑100 (MQ=255)
                                                                      atAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc       >  1:2830830/2‑100 (MQ=255)
                                                                       ttggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3461233/5‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATTATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:4268755/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTCTTATACTTATTGGCt      >  1:5193603/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:4476254/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:1064497/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:941956/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:765547/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:4675653/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:4870483/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:5774282/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:5596535/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:5297944/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:5461849/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:1247442/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:1896336/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:2688109/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:2718848/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:2742089/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:2766369/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3033636/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3103044/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3419428/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3508432/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3675463/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3758277/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3895990/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3947907/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:4154493/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:1416662/1‑100 (MQ=255)
                                                                       tAGGCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:1338929/1‑100 (MQ=255)
                                                                       caggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:3616974/5‑100 (MQ=255)
                                                                       caggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:5050450/5‑100 (MQ=255)
                                                                       caggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt      >  1:4833975/5‑100 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:1638235/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:1808082/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:1598232/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:4441877/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:4582633/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:423765/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:4292293/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt     >  1:4337359/4‑99 (MQ=255)
                                                                        aggcGCAGAGAGGAGGTGCATTACCTTGCTAATCATTTTCGTTCAAATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGAATTATAATTATTGGCtt     >  1:1850883/4‑99 (MQ=255)
                                                                         ggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta    >  1:3825358/3‑98 (MQ=255)
                                                                         ggcGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta    >  1:1214177/3‑98 (MQ=255)
                                                                         caCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTag    >  1:686856/2‑99 (MQ=255)
                                                                          aCGCAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg   >  1:2907845/1‑100 (MQ=255)
                                                                                               |||                                                                             
AAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGT  >  NC_007793/1929021‑1929194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑