| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,632,339 | 2 bp→AA | coding (88‑89/357 nt) | SAUSA300_RS08070 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,632,339 | 0 | G | A | 81.2% | 24.2 / ‑0.3 | 16 | T30I (ACA→ATA) | SAUSA300_RS08070 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (4/9); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_007793 | 1,632,340 | 0 | T | A | 87.5% | 35.9 / ‑1.3 | 16 | T30S (ACA→TCA) | SAUSA300_RS08070 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (4/10); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CAACATTTTTATACTTCTTTGTATTTTTCACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATAA > NC_007793/1632280‑1632432 || cAACATTTTTATACTTCTTTGTATTTTTCACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGc < 1:4721570/100‑1 (MQ=255) tcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATa > 1:2390683/7‑100 (MQ=255) tcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATa > 1:2946612/7‑100 (MQ=255) cactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATAc > 1:4568314/6‑100 (MQ=255) actcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACa < 1:2683281/96‑1 (MQ=255) ctcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaa > 1:2131754/4‑100 (MQ=255) ctcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaa < 1:5736548/97‑1 (MQ=255) aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc < 1:1147150/100‑1 (MQ=255) aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc < 1:2204886/100‑1 (MQ=255) aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc < 1:2720558/100‑1 (MQ=255) aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc < 1:2887236/100‑1 (MQ=255) aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc < 1:484466/100‑1 (MQ=255) aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc < 1:521497/100‑1 (MQ=255) tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa < 1:4389652/100‑1 (MQ=255) tttCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCtagt < 1:2599159/100‑4 (MQ=255) gaatttGAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATaa < 1:3280461/97‑1 (MQ=255) || CAACATTTTTATACTTCTTTGTATTTTTCACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATAA > NC_007793/1632280‑1632432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |