Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,585,819	2 bp→CG	coding (288‑289/303 nt)	SAUSA300_RS07790 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,585,819	0	T	C	100.0%	20.4 / NA	10	N97D (AAC→GAC)	SAUSA300_RS07790	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/8);  total (2/8)
*	NC_007793	1,585,820	0	A	G	100.0%	20.9 / NA	10	D96D (GAT→GAC)	SAUSA300_RS07790	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/8);  total (2/8)

CTCACCAAATTTGTAATACATTGTTTTAGCCCTCCTTGTTATCATCAATACCGTGAAATTTTTGTGATTTACACATTTGGAGAACATTGACAATGTCTTTATAACTCTTAGTGCTATCCAATAAGGAAGCAA  >  NC_007793/1585780‑1585911                                        ||                                                                                            gtcacccgttttGTAATACATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCttt                                  >  1:3259601/10‑100 (MQ=18)                   cATTGTTTTAGTCCTCCTTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATc                >  1:2214343/1‑100 (MQ=255)                                gatctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa   <  1:2865473/97‑1 (MQ=255)                                gatctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa   <  1:4950536/97‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa   <  1:2790828/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa   <  1:3312560/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa   <  1:415331/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa   <  1:5462929/100‑1 (MQ=255)                                ctcctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTAAAAAGGGGGAGAACATTGACAATCTCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCa   <  1:228697/100‑1 (MQ=255)                                 atctTGTCGTCATCTATACCGAGAATTTTTTGTGATTTACACATTTGGAGAACATTGACAATATCTTTATAACTCTTAGTGCTATCCAATAAGTAAGCaa  <  1:641682/98‑1 (MQ=255)                                        ||                                                                                            CTCACCAAATTTGTAATACATTGTTTTAGCCCTCCTTGTTATCATCAATACCGTGAAATTTTTGTGATTTACACATTTGGAGAACATTGACAATGTCTTTATAACTCTTAGTGCTATCCAATAAGGAAGCAA  >  NC_007793/1585780‑1585911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑