Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 656,344 2 bp→TT coding (127‑128/630 nt) SAUSA300_RS03115 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793656,3440AT100.0% 47.1 / NA 20N43Y (AAT→TAT) SAUSA300_RS03115hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/14);  total (6/14)
*NC_007793656,3450AT100.0% 47.2 / NA 20N43I (AAT→ATT) SAUSA300_RS03115hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/14);  total (6/14)

TATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGAATAGTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGC  >  NC_007793/656257‑656438
                                                                                       ||                                                                                             
tataGAATTATTAAATACAGTAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCaaaa                                                                                    <  1:5044518/100‑1 (MQ=17)
                     aatGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTagag                                                               <  1:514361/100‑1 (MQ=255)
                                cATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAgaagagaagatcggaag                                                    >  1:5481655/1‑93 (MQ=17)
                                            agtaagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTCAAagatc                                        >  1:5288101/1‑99 (MQ=21)
                                            agtaagtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGCACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATAtt                                        >  1:4241892/1‑100 (MQ=21)
                                               aagtaCTTGGATTGCTTATTTTTTGCCCATGGTTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTc                                     <  1:2285672/100‑1 (MQ=25)
                                                agtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCa                                    <  1:3835638/100‑1 (MQ=255)
                                                agtaCTTGGATTGCTTA‑TTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCac                                   <  1:458771/100‑2 (MQ=21)
                                                 gtaCTTGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCaa                                   <  1:293232/100‑1 (MQ=255)
                                                      tGGATTGCTTATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCg                              <  1:2006569/100‑1 (MQ=255)
                                                               tATTTTTTGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAaata                     >  1:4877716/1‑100 (MQ=255)
                                                                      tGCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACttt              >  1:2015108/1‑100 (MQ=255)
                                                                       gCCCATGATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTc             >  1:4296571/1‑100 (MQ=255)
                                                                             gATTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCt       <  1:2235716/100‑1 (MQ=255)
                                                                              aTTAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCta      <  1:2430232/100‑2 (MQ=255)
                                                                               ttAATTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGt     <  1:2128919/100‑1 (MQ=255)
                                                                                 aaTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTg   <  1:3991730/100‑1 (MQ=255)
                                                                                  aTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc  <  1:3018277/100‑1 (MQ=255)
                                                                                  aTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc  <  1:2454011/100‑1 (MQ=255)
                                                                                  aTTGGTTTATTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGACAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGc  <  1:681961/100‑1 (MQ=21)
                                                                                       ||                                                                                             
TATAGAATTATTAAATACAATAATGAATATTTCATGGTTGATTTAGTAAGTACTTGGATTGCTTATTTTTTGCCCATGATTAATTGGAATAGTCCCAAAAAGTACGCGAAAATTAGTAGAGAAGAATTTGAAAGTTTAAATATTGTCAAACCCGCTAAAAATAATACTTTCTGGCCTGTTGC  >  NC_007793/656257‑656438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑