Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,712,958	G→A	intergenic (‑202/+406)	SAUSA300_RS13915 ← / ← SAUSA300_RS13920	transglycosylase IsaA/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,712,958	0	G	A	100.0%	14.2 / NA	7	intergenic (‑202/+406)	SAUSA300_RS13915/SAUSA300_RS13920	transglycosylase IsaA/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/5);  total (2/5)

TACTCACTATATCACAAAAAACCAGCCAGTAAATTACACTTTCTTTACAAAACATTACAATATCAAGTGTTATTTGTAATGTTGAAATATGGCTGTTTTATACTGTAATGTGAAATATGTGCCCTTTAGAATCCAATCAACCCTTGAAATAGTCTTTAACACATA  >  NC_007793/2712864‑2713028                                                                                               |                                                                       aaCTCACTATATCACAAAAAACCAGCCAGTAAATTACACTTTCTTTACAAAACATTACAATATCAAGTGTTATTTGTAATGTTGAAATATGGCTATTTta                                                                   >  1:5289576/2‑100 (MQ=255)                                         ttctttACAAAACATTACAATATCAAGTGTTATTTGTAATGTTGAAATATGGCTATTTTATACTGTAATGTGAAATATGTGCCCTTTAGAATCCAATCaa                           >  1:3124030/1‑100 (MQ=255)                                               aCAAAACATAACAATATCAAGTGTTATTTGTAATGTTGAAATATGGCTATTTTATACTGTAATGTGAAATATGTGCCCTTTAGAATCCAATCAACCCTTg                     <  1:1137033/100‑1 (MQ=255)                                                                  aGTGTTATTTGTAATGTTGAAATATGGCTATTTTATACTGTAATGTGAAATATGTGCCCTTTAGAATCCAGTCAACCCTTGAAATAGTCTTTAACACATa  <  1:5401212/100‑1 (MQ=255)                                                                  aGTGTTATTTGTAATGTTGAAATATGGCTATTTTATACTGTAATGTGAAATATGTGCCCTTTAGAATCCAATCAACCCTTGAAATAGTCTTTAACACATa  <  1:2891977/100‑1 (MQ=255)                                                                  aGTGTTATTTGTAATGTTGAAATATGGCTATTTTATACTGTAATGTGAAATATGTGCCCTTTAGAATCCAATCAACCCTTGAAATAGTCTTTAACACATa  <  1:75976/100‑1 (MQ=255)                                                                  aGTGTGATTTGTAATGTGGAAATATGGCTATTTTATACTGTAATTTGAAATTTGGGCCCGTTAGAATCCAATCAACCCTTGAAATAGTCTTTAACACATa  <  1:5440694/100‑1 (MQ=255)                                                                                               |                                                                       TACTCACTATATCACAAAAAACCAGCCAGTAAATTACACTTTCTTTACAAAACATTACAATATCAAGTGTTATTTGTAATGTTGAAATATGGCTGTTTTATACTGTAATGTGAAATATGTGCCCTTTAGAATCCAATCAACCCTTGAAATAGTCTTTAACACATA  >  NC_007793/2712864‑2713028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑