Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,685,939	T→G	S249A (TCT→GCT)	SAUSA300_RS13805 →	ATP‑dependent Clp protease ATP‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,685,939	0	T	G	100.0%	122.6 / NA	44	S249A (TCT→GCT)	SAUSA300_RS13805	ATP‑dependent Clp protease ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/2);  total (42/2)

CTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATTCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTCGCGAAAA  >  NC_007793/2685847‑2686027                                                                                             |                                                                                         cTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCAtt                                                                                   >  1:243660/1‑100 (MQ=255)   gATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTaa                                                                                 >  1:4585224/1‑100 (MQ=255)        tttGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACgcgc                                                                            <  1:4925883/100‑1 (MQ=255)            aaaCCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGAt                                                                        >  1:1683563/1‑100 (MQ=255)              atcTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGAttt                                                                      >  1:2950905/3‑100 (MQ=255)              aCCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACgcgccagatc                                                                      >  1:4362509/1‑94 (MQ=255)               tgtGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTa                                                                     >  1:3042056/3‑100 (MQ=255)               gcTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTa                                                                     >  1:2727440/2‑100 (MQ=255)               ccTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTa                                                                     >  1:5284282/1‑100 (MQ=255)                cTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTaa                                                                    >  1:3228488/1‑100 (MQ=255)                  gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                  >  1:199/1‑100 (MQ=255)                  gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                  >  1:1842319/1‑100 (MQ=255)                  gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                  >  1:1674814/1‑100 (MQ=255)                  gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                  >  1:5315185/1‑100 (MQ=255)                  gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                  >  1:1040160/1‑100 (MQ=255)                  gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                  >  1:3166604/1‑100 (MQ=255)                         atCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                                           >  1:418708/2‑100 (MQ=255)                         atCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                                           >  1:5083876/2‑100 (MQ=255)                          tCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCtt                                                          >  1:3235275/1‑100 (MQ=255)                          acGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCtt                                                          >  1:2790666/2‑100 (MQ=255)                           cGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTg                                                         >  1:872225/1‑100 (MQ=255)                            gtggtgAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGt                                                        >  1:2421064/1‑100 (MQ=255)                             tggtgAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCGGATTTAATGAAGTGCTTGtt                                                       <  1:1663728/100‑1 (MQ=255)                              ggtgAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTa                                                      >  1:4852013/1‑100 (MQ=255)                                        cTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAg                                            >  1:147354/1‑100 (MQ=255)                                        cTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAg                                            >  1:393404/1‑100 (MQ=255)                                          attattGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAgcg                                          >  1:3310541/1‑100 (MQ=255)                                          attattGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAgcg                                          >  1:3520750/1‑100 (MQ=255)                                                 atGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGa                                   >  1:4706148/2‑100 (MQ=255)                                                  tGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGAt                                  >  1:272280/1‑100 (MQ=255)                                                  tGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGAt                                  >  1:3819976/1‑100 (MQ=255)                                                   gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa                                 >  1:1038433/1‑100 (MQ=255)                                                   gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa                                 >  1:5302900/1‑100 (MQ=255)                                                   gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa                                 >  1:172865/1‑100 (MQ=255)                                                   gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa                                 >  1:4307502/1‑100 (MQ=255)                                                   gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa                                 >  1:421600/1‑100 (MQ=255)                                                      acaacaCAAGGTGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                              >  1:867162/1‑100 (MQ=255)                                                      acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                              >  1:1370584/1‑100 (MQ=255)                                                      acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCAGTAACGCGCAGATTTAATGAAGAGCTTGTTAATGAACCAAGCGCTAAAGATACTg                              >  1:4986070/1‑100 (MQ=255)                                                       caacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGt                             >  1:3102421/1‑100 (MQ=255)                                                       aaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGt                             >  1:366931/2‑100 (MQ=255)                                                                      atCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAgg              >  1:4261590/1‑100 (MQ=255)                                                                                 atTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTcgcgtca   >  1:2322329/1‑97 (MQ=255)                                                                                  cccTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTCACGaaga  >  1:3956705/3‑98 (MQ=255)                                                                                             |                                                                                         CTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATTCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTCGCGAAAA  >  NC_007793/2685847‑2686027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑