Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,655,119	G→A	A84V (GCT→GTT)	SAUSA300_RS13630 ←	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,655,119	0	G	A	100.0%	13.6 / NA	7	A84V (GCT→GTT)	SAUSA300_RS13630	carboxylesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/6);  total (1/6)

ATTTGATCCATTTGAAAATCCAACAAGTACTGCTTTTTCAATATCAAAATCATAACGTTCAGCAGCTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTG  >  NC_007793/2655055‑2655199                                                                 |                                                                                 atTTGATCCATTTGAAAATCCAACAAGTACTGCTTTTTCAATATCAAAATCATAACGTTCAGCAACTTCTTTAATGAACGTCAACAACTCTTGTCCACGa                                               <  1:4147943/100‑1 (MQ=255)       tCCATTTGAAAATCCAACAAGTACTGCTTTTTCAATATCAAAATCATAACGTTCAGCAACTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGcc                                         <  1:5538822/100‑1 (MQ=255)        ccATTTGAAAATCCAACAAGTACTGCTTTTTCAATATCAAAATCATAACGTTCAGCAACTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCa                                        <  1:3638432/100‑1 (MQ=255)                  tcccAACAAGTACTGCTTTTTCAATATCAAAATCATAACGTTCAGCAACTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCttcttc                              >  1:2104867/3‑100 (MQ=255)                                    tttCAATATCAAAATCATAACGTTCAGCAACTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCAcc            <  1:3355798/100‑1 (MQ=255)                                              aaaaTCATAACGTTCAGCAACTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTg  <  1:5187869/100‑1 (MQ=255)                                              aaaaTCATAACGTTCAGCAACTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTg  <  1:5655988/100‑1 (MQ=255)                                                                 |                                                                                 ATTTGATCCATTTGAAAATCCAACAAGTACTGCTTTTTCAATATCAAAATCATAACGTTCAGCAGCTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTG  >  NC_007793/2655055‑2655199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑