Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,627,848	G→C	G203A (GGT→GCT)	SAUSA300_RS13515 →	transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,627,848	0	G	C	100.0%	11.2 / NA	6	G203A (GGT→GCT)	SAUSA300_RS13515	transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (1/5);  total (1/5)

AATTTAAAAGATTTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAATAATAAAGGTATGCTAATCAAAGAACGATCCCATGCAGATGAAAGGATTGTGTTAATCAAAATAAATAAAATACAATATAACACTATTAAAAGCATATTC  >  NC_007793/2627769‑2627939                                                                                |                                                                                            aaTTTAAAAGATTTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAATAATAAAGCTATGCTAATCAAAGAACGag                                                                         <  1:911656/100‑2 (MQ=255)        aaGATTTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAATAATAAAGCTATGCTAATCAAAGAACGATCCCTTGc                                                                  <  1:4016943/100‑1 (MQ=255)        aaGATTTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAATAATAAAGCTATGCTAATCAAAGAACGATCCCTTGc                                                                  <  1:4542318/100‑1 (MQ=255)        aaGATTTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAATAATAAAGCTATGCTAATCAAAGAACGATCCCTTGc                                                                  <  1:524525/100‑1 (MQ=255)                                           cAAATTGTTAGGTCAGTTAATAGATTAAATAATAAAGCTATGCTAATCAAAGAACGATCCCTTGCAGATGAAAGGATTGTGTTAATCAAAATaaataaaa                               >  1:4268075/1‑100 (MQ=255)                                                                        taataaAGCTATGCTAATCAAAGAACGATCCCTTGCTGATGAAAGGATTGTGTTAATCAAAATAAATAAAATACAATATAACACTATTAAAAGCATATTc  <  1:982336/100‑1 (MQ=255)                                                                                |                                                                                            AATTTAAAAGATTTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAATAATAAAGGTATGCTAATCAAAGAACGATCCCATGCAGATGAAAGGATTGTGTTAATCAAAATAAATAAAATACAATATAACACTATTAAAAGCATATTC  >  NC_007793/2627769‑2627939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑