Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,601,699	T→C	intergenic (‑29/+234)	SAUSA300_RS13370 ← / ← SAUSA300_RS13375	hypothetical protein/short‑chain dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,601,699	0	T	C	100.0%	43.6 / NA	17	intergenic (‑29/+234)	SAUSA300_RS13370/SAUSA300_RS13375	hypothetical protein/short‑chain dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/15);  total (2/15)

CCAATTTTATCTGCAATCTCTCTTATAGCTTGATATAGCAATCTAAAACTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGATAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTACAAT  >  NC_007793/2601603‑2601775                                                                                                 |                                                                             ccAATTTTATCTGCAATCTCTCTTATAGCTTGATATAGCAATCTAAAACTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGACaaa                                                                           >  1:5561636/1‑100 (MQ=255)           cTGCAATCTCTCTTATAGCTTGATATAGCAATCTAAAACTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTa                                                                 <  1:1141204/100‑1 (MQ=255)                 tctctcTTGTAGCTTGATATAGCAATCTAAAACTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTaa                                                           <  1:437677/100‑1 (MQ=255)                                         aTCTAAAACTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAGCTCg                                   <  1:1429084/100‑1 (MQ=255)                                         aTCTAAAACTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCg                                   <  1:1492281/100‑1 (MQ=255)                                         aTCTAAAACTTTCTTTTGCTTTTAACATAAAATTCTCCTTAAGTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCg                                   <  1:4379644/100‑1 (MQ=255)                                             aaaaCTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGAt                               >  1:4752769/1‑100 (MQ=255)                                                                 aCATAAAATTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACg           <  1:2065064/100‑1 (MQ=255)                                                                 aCATAAAATTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACg           <  1:5339268/100‑1 (MQ=255)                                                                       cgatctcCTTAGTTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTAc     <  1:3418299/97‑1 (MQ=255)                                                                       aaTTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTAc     <  1:114839/100‑1 (MQ=255)                                                                       aaTTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTAc     <  1:2069951/100‑1 (MQ=255)                                                                        aTTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTACa    <  1:3583747/100‑1 (MQ=255)                                                                        aTTCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTACa    <  1:1816886/100‑1 (MQ=255)                                                                         ttCTCCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTACac   <  1:5147420/100‑2 (MQ=255)                                                                          tctcCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTACAAt  <  1:2843780/100‑1 (MQ=255)                                                                          tctcCTTAATTTATTAGAATAGACAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTACAAt  <  1:2781615/100‑1 (MQ=255)                                                                                                 |                                                                             CCAATTTTATCTGCAATCTCTCTTATAGCTTGATATAGCAATCTAAAACTTTCTTTTTCTTTTAACATAAAATTCTCCTTAATTTATTAGAATAGATAAAATACAGCTTATTTTAAAGATACTCCAAAAGTTTGAACTCGAGATTCTGATAAATTAGATGCACGACCTACAAT  >  NC_007793/2601603‑2601775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑